Cargando…

17β-estradiol upregulates oxytocin and the oxytocin receptor in C2C12 myotubes

BACKGROUND: The endocrinology of skeletal muscle is highly complex and many issues about hormone action in skeletal muscle are still unresolved. Aim of the work is to improve our knowledge on the relationship between skeletal muscle and 17β-estradiol. METHODS: The skeletal muscle cell line C2C12 was...

Descripción completa

Detalles Bibliográficos
Autores principales: Berio, Enrica, Divari, Sara, Starvaggi Cucuzza, Laura, Biolatti, Bartolomeo, Cannizzo, Francesca Tiziana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376115/
https://www.ncbi.nlm.nih.gov/pubmed/28382233
http://dx.doi.org/10.7717/peerj.3124
_version_ 1782519107532357632
author Berio, Enrica
Divari, Sara
Starvaggi Cucuzza, Laura
Biolatti, Bartolomeo
Cannizzo, Francesca Tiziana
author_facet Berio, Enrica
Divari, Sara
Starvaggi Cucuzza, Laura
Biolatti, Bartolomeo
Cannizzo, Francesca Tiziana
author_sort Berio, Enrica
collection PubMed
description BACKGROUND: The endocrinology of skeletal muscle is highly complex and many issues about hormone action in skeletal muscle are still unresolved. Aim of the work is to improve our knowledge on the relationship between skeletal muscle and 17β-estradiol. METHODS: The skeletal muscle cell line C2C12 was treated with 17β-estradiol, the oxytocin peptide and a combination of the two hormones. The mRNA levels of myogenic regulatory factors, myosin heavy chain, oxytocin, oxytocin receptor and adipogenic factors were analysed in C2C12 myotubes. RESULTS: It was demonstrated that C2C12 myoblasts and myotubes express oxytocin and its receptor, in particular the receptor levels physiologically increase in differentiated myotubes. Myotubes treated with 17β-estradiol overexpressed oxytocin and oxytocin receptor genes by approximately 3- and 29-fold, respectively. A decrease in the expression of fatty acid binding protein 4 (0.62-fold), a fat metabolism-associated gene, was observed in oxytocin-treated myotubes. On the contrary, fatty acid binding protein 4 was upregulated (2.66-fold) after the administration of the combination of 17β-estradiol and oxytocin. 17β-estradiol regulates oxytocin and its receptor in skeletal muscle cells and they act in a synergic way on fatty acid metabolism. DISCUSSION: Oxytocin and its receptor are physiologically regulated along differentiation. 17β-estradiol regulates oxytocin and its receptor in skeletal muscle cells. 17β-estradiol and oxytocin act in a synergic way on fatty acid metabolism. A better understanding of the regulation of skeletal muscle homeostasis by estrogens and oxytocin peptide could contribute to increase our knowledge of muscle and its metabolism.
format Online
Article
Text
id pubmed-5376115
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-53761152017-04-05 17β-estradiol upregulates oxytocin and the oxytocin receptor in C2C12 myotubes Berio, Enrica Divari, Sara Starvaggi Cucuzza, Laura Biolatti, Bartolomeo Cannizzo, Francesca Tiziana PeerJ Cell Biology BACKGROUND: The endocrinology of skeletal muscle is highly complex and many issues about hormone action in skeletal muscle are still unresolved. Aim of the work is to improve our knowledge on the relationship between skeletal muscle and 17β-estradiol. METHODS: The skeletal muscle cell line C2C12 was treated with 17β-estradiol, the oxytocin peptide and a combination of the two hormones. The mRNA levels of myogenic regulatory factors, myosin heavy chain, oxytocin, oxytocin receptor and adipogenic factors were analysed in C2C12 myotubes. RESULTS: It was demonstrated that C2C12 myoblasts and myotubes express oxytocin and its receptor, in particular the receptor levels physiologically increase in differentiated myotubes. Myotubes treated with 17β-estradiol overexpressed oxytocin and oxytocin receptor genes by approximately 3- and 29-fold, respectively. A decrease in the expression of fatty acid binding protein 4 (0.62-fold), a fat metabolism-associated gene, was observed in oxytocin-treated myotubes. On the contrary, fatty acid binding protein 4 was upregulated (2.66-fold) after the administration of the combination of 17β-estradiol and oxytocin. 17β-estradiol regulates oxytocin and its receptor in skeletal muscle cells and they act in a synergic way on fatty acid metabolism. DISCUSSION: Oxytocin and its receptor are physiologically regulated along differentiation. 17β-estradiol regulates oxytocin and its receptor in skeletal muscle cells. 17β-estradiol and oxytocin act in a synergic way on fatty acid metabolism. A better understanding of the regulation of skeletal muscle homeostasis by estrogens and oxytocin peptide could contribute to increase our knowledge of muscle and its metabolism. PeerJ Inc. 2017-03-30 /pmc/articles/PMC5376115/ /pubmed/28382233 http://dx.doi.org/10.7717/peerj.3124 Text en ©2017 Berio et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Cell Biology
Berio, Enrica
Divari, Sara
Starvaggi Cucuzza, Laura
Biolatti, Bartolomeo
Cannizzo, Francesca Tiziana
17β-estradiol upregulates oxytocin and the oxytocin receptor in C2C12 myotubes
title 17β-estradiol upregulates oxytocin and the oxytocin receptor in C2C12 myotubes
title_full 17β-estradiol upregulates oxytocin and the oxytocin receptor in C2C12 myotubes
title_fullStr 17β-estradiol upregulates oxytocin and the oxytocin receptor in C2C12 myotubes
title_full_unstemmed 17β-estradiol upregulates oxytocin and the oxytocin receptor in C2C12 myotubes
title_short 17β-estradiol upregulates oxytocin and the oxytocin receptor in C2C12 myotubes
title_sort 17β-estradiol upregulates oxytocin and the oxytocin receptor in c2c12 myotubes
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376115/
https://www.ncbi.nlm.nih.gov/pubmed/28382233
http://dx.doi.org/10.7717/peerj.3124
work_keys_str_mv AT berioenrica 17bestradiolupregulatesoxytocinandtheoxytocinreceptorinc2c12myotubes
AT divarisara 17bestradiolupregulatesoxytocinandtheoxytocinreceptorinc2c12myotubes
AT starvaggicucuzzalaura 17bestradiolupregulatesoxytocinandtheoxytocinreceptorinc2c12myotubes
AT biolattibartolomeo 17bestradiolupregulatesoxytocinandtheoxytocinreceptorinc2c12myotubes
AT cannizzofrancescatiziana 17bestradiolupregulatesoxytocinandtheoxytocinreceptorinc2c12myotubes