Direct Detection of FoxP3 Expression in Thymic Double-Negative CD4(−)CD8(−) Cells by Flow Cytometry

Foxp3 expression is a marker of regulatory T cells (T(reg)), but how early it is expressed in the thymus is still not fully defined. In this study, we examined Foxp3 expression in double-negative (DN) CD4(−)CD8(−) T cell precursors in the thymus by flow cytometry. By increasing the number of collect...

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Autores principales: Liu, Gang, Li, Zongfang, Wei, Yang, Lin, Yan, Yang, Cengceng, Liu, Tie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376166/
https://www.ncbi.nlm.nih.gov/pubmed/25060864
http://dx.doi.org/10.1038/srep05781
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author Liu, Gang
Li, Zongfang
Wei, Yang
Lin, Yan
Yang, Cengceng
Liu, Tie
author_facet Liu, Gang
Li, Zongfang
Wei, Yang
Lin, Yan
Yang, Cengceng
Liu, Tie
author_sort Liu, Gang
collection PubMed
description Foxp3 expression is a marker of regulatory T cells (T(reg)), but how early it is expressed in the thymus is still not fully defined. In this study, we examined Foxp3 expression in double-negative (DN) CD4(−)CD8(−) T cell precursors in the thymus by flow cytometry. By increasing the number of collected cells from the conventional 10(4) cells up to more than 10(6) cells during flow cytometry, we found that DN cells exhibited higher Foxp3 expression than double-positive (DP) CD4(+)CD8(+) and single-positive (SP) CD4(+) or CD8(+) (SP) T cells. CD44(+) expression positively correlated with Foxp3 in thymic DN cells. Furthermore, TCR-β(−)CD25(+) DN cells exhibited the highest frequency of Foxp3-expressing cells. Almost all Foxp3(+) cells expressed CD25in DN cells. These results suggest that Foxp3 expression in DN cells can directly be detected by flow cytometry and it was positively corelated with CD25 and CD44 in DN cells.
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spelling pubmed-53761662017-04-03 Direct Detection of FoxP3 Expression in Thymic Double-Negative CD4(−)CD8(−) Cells by Flow Cytometry Liu, Gang Li, Zongfang Wei, Yang Lin, Yan Yang, Cengceng Liu, Tie Sci Rep Article Foxp3 expression is a marker of regulatory T cells (T(reg)), but how early it is expressed in the thymus is still not fully defined. In this study, we examined Foxp3 expression in double-negative (DN) CD4(−)CD8(−) T cell precursors in the thymus by flow cytometry. By increasing the number of collected cells from the conventional 10(4) cells up to more than 10(6) cells during flow cytometry, we found that DN cells exhibited higher Foxp3 expression than double-positive (DP) CD4(+)CD8(+) and single-positive (SP) CD4(+) or CD8(+) (SP) T cells. CD44(+) expression positively correlated with Foxp3 in thymic DN cells. Furthermore, TCR-β(−)CD25(+) DN cells exhibited the highest frequency of Foxp3-expressing cells. Almost all Foxp3(+) cells expressed CD25in DN cells. These results suggest that Foxp3 expression in DN cells can directly be detected by flow cytometry and it was positively corelated with CD25 and CD44 in DN cells. Nature Publishing Group 2014-07-25 /pmc/articles/PMC5376166/ /pubmed/25060864 http://dx.doi.org/10.1038/srep05781 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liu, Gang
Li, Zongfang
Wei, Yang
Lin, Yan
Yang, Cengceng
Liu, Tie
Direct Detection of FoxP3 Expression in Thymic Double-Negative CD4(−)CD8(−) Cells by Flow Cytometry
title Direct Detection of FoxP3 Expression in Thymic Double-Negative CD4(−)CD8(−) Cells by Flow Cytometry
title_full Direct Detection of FoxP3 Expression in Thymic Double-Negative CD4(−)CD8(−) Cells by Flow Cytometry
title_fullStr Direct Detection of FoxP3 Expression in Thymic Double-Negative CD4(−)CD8(−) Cells by Flow Cytometry
title_full_unstemmed Direct Detection of FoxP3 Expression in Thymic Double-Negative CD4(−)CD8(−) Cells by Flow Cytometry
title_short Direct Detection of FoxP3 Expression in Thymic Double-Negative CD4(−)CD8(−) Cells by Flow Cytometry
title_sort direct detection of foxp3 expression in thymic double-negative cd4(−)cd8(−) cells by flow cytometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376166/
https://www.ncbi.nlm.nih.gov/pubmed/25060864
http://dx.doi.org/10.1038/srep05781
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