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Selective detection of endogenous H(2)S in living cells and the mouse hippocampus using a ratiometric fluorescent probe
As one of three gasotransmitters, the fundamental signalling roles of hydrogen sulphide are receiving increasing attention. New tools for the accurate detection of hydrogen sulphide in cells and tissues are in demand to probe its biological functions. We report the p-nitrobenzyl-based ratiometric fl...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376197/ https://www.ncbi.nlm.nih.gov/pubmed/25070356 http://dx.doi.org/10.1038/srep05870 |
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author | Zhang, Ling Meng, Wen-qi Lu, Liang Xue, Yun-Sheng Li, Cheng Zou, Fang Liu, Yi Zhao, Jing |
author_facet | Zhang, Ling Meng, Wen-qi Lu, Liang Xue, Yun-Sheng Li, Cheng Zou, Fang Liu, Yi Zhao, Jing |
author_sort | Zhang, Ling |
collection | PubMed |
description | As one of three gasotransmitters, the fundamental signalling roles of hydrogen sulphide are receiving increasing attention. New tools for the accurate detection of hydrogen sulphide in cells and tissues are in demand to probe its biological functions. We report the p-nitrobenzyl-based ratiometric fluorescent probe RHP-2, which features a low detection limit, high selectivity and good photostability. The emission intensity ratios had a good linear relationship with the sulphide concentrations in PBS buffer and bovine serum. Our probe was applied to the ratiometric determination and imaging of endogenous H(2)S in living cells. Furthermore, RHP-2 was used as an effective tool to measure endogenous H(2)S in the mouse hippocampus. We observed a significant reduction in sulphide concentrations and downregulated expression of cystathionine β-synthetase (CBS) mRNA and CBS protein in the mouse hippocampus in a chronic unpredictable mild stress (CUMS)-induced depression model. These data suggested that decreased concentrations of endogenous H(2)S may be involved in the pathogenesis of chronic stress depression. |
format | Online Article Text |
id | pubmed-5376197 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53761972017-04-03 Selective detection of endogenous H(2)S in living cells and the mouse hippocampus using a ratiometric fluorescent probe Zhang, Ling Meng, Wen-qi Lu, Liang Xue, Yun-Sheng Li, Cheng Zou, Fang Liu, Yi Zhao, Jing Sci Rep Article As one of three gasotransmitters, the fundamental signalling roles of hydrogen sulphide are receiving increasing attention. New tools for the accurate detection of hydrogen sulphide in cells and tissues are in demand to probe its biological functions. We report the p-nitrobenzyl-based ratiometric fluorescent probe RHP-2, which features a low detection limit, high selectivity and good photostability. The emission intensity ratios had a good linear relationship with the sulphide concentrations in PBS buffer and bovine serum. Our probe was applied to the ratiometric determination and imaging of endogenous H(2)S in living cells. Furthermore, RHP-2 was used as an effective tool to measure endogenous H(2)S in the mouse hippocampus. We observed a significant reduction in sulphide concentrations and downregulated expression of cystathionine β-synthetase (CBS) mRNA and CBS protein in the mouse hippocampus in a chronic unpredictable mild stress (CUMS)-induced depression model. These data suggested that decreased concentrations of endogenous H(2)S may be involved in the pathogenesis of chronic stress depression. Nature Publishing Group 2014-07-29 /pmc/articles/PMC5376197/ /pubmed/25070356 http://dx.doi.org/10.1038/srep05870 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | Article Zhang, Ling Meng, Wen-qi Lu, Liang Xue, Yun-Sheng Li, Cheng Zou, Fang Liu, Yi Zhao, Jing Selective detection of endogenous H(2)S in living cells and the mouse hippocampus using a ratiometric fluorescent probe |
title | Selective detection of endogenous H(2)S in living cells and the mouse hippocampus using a ratiometric fluorescent probe |
title_full | Selective detection of endogenous H(2)S in living cells and the mouse hippocampus using a ratiometric fluorescent probe |
title_fullStr | Selective detection of endogenous H(2)S in living cells and the mouse hippocampus using a ratiometric fluorescent probe |
title_full_unstemmed | Selective detection of endogenous H(2)S in living cells and the mouse hippocampus using a ratiometric fluorescent probe |
title_short | Selective detection of endogenous H(2)S in living cells and the mouse hippocampus using a ratiometric fluorescent probe |
title_sort | selective detection of endogenous h(2)s in living cells and the mouse hippocampus using a ratiometric fluorescent probe |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376197/ https://www.ncbi.nlm.nih.gov/pubmed/25070356 http://dx.doi.org/10.1038/srep05870 |
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