A tetrameric kinesin, Kif25, suppresses centrosome separation to establish proper spindle orientation

Microtubules tether centrosomes together during interphase (1). How this is accomplished and what benefit it provides to the cell is not known. We have identified a bipolar, minus-end directed kinesin, Kif25, that suppresses centrosome separation. Kif25 is required to prevent premature centrosome se...

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Autores principales: Decarreau, Justin, Wagenbach, Michael, Lynch, Eric, Halpern, Aaron R., Vaughan, Joshua C., Kollman, Justin, Wordeman, Linda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376238/
https://www.ncbi.nlm.nih.gov/pubmed/28263957
http://dx.doi.org/10.1038/ncb3486
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author Decarreau, Justin
Wagenbach, Michael
Lynch, Eric
Halpern, Aaron R.
Vaughan, Joshua C.
Kollman, Justin
Wordeman, Linda
author_facet Decarreau, Justin
Wagenbach, Michael
Lynch, Eric
Halpern, Aaron R.
Vaughan, Joshua C.
Kollman, Justin
Wordeman, Linda
author_sort Decarreau, Justin
collection PubMed
description Microtubules tether centrosomes together during interphase (1). How this is accomplished and what benefit it provides to the cell is not known. We have identified a bipolar, minus-end directed kinesin, Kif25, that suppresses centrosome separation. Kif25 is required to prevent premature centrosome separation during interphase. We show that premature centrosome separation leads to microtubule-dependent nuclear translocation, culminating in eccentric nuclear positioning that disrupts the cortical spindle positioning machinery. Kif25’s activity during interphase is required to maintain a centered nucleus to ensure the spindle is stably oriented at the onset of mitosis.
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spelling pubmed-53762382017-09-06 A tetrameric kinesin, Kif25, suppresses centrosome separation to establish proper spindle orientation Decarreau, Justin Wagenbach, Michael Lynch, Eric Halpern, Aaron R. Vaughan, Joshua C. Kollman, Justin Wordeman, Linda Nat Cell Biol Article Microtubules tether centrosomes together during interphase (1). How this is accomplished and what benefit it provides to the cell is not known. We have identified a bipolar, minus-end directed kinesin, Kif25, that suppresses centrosome separation. Kif25 is required to prevent premature centrosome separation during interphase. We show that premature centrosome separation leads to microtubule-dependent nuclear translocation, culminating in eccentric nuclear positioning that disrupts the cortical spindle positioning machinery. Kif25’s activity during interphase is required to maintain a centered nucleus to ensure the spindle is stably oriented at the onset of mitosis. 2017-03-06 2017-04 /pmc/articles/PMC5376238/ /pubmed/28263957 http://dx.doi.org/10.1038/ncb3486 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Decarreau, Justin
Wagenbach, Michael
Lynch, Eric
Halpern, Aaron R.
Vaughan, Joshua C.
Kollman, Justin
Wordeman, Linda
A tetrameric kinesin, Kif25, suppresses centrosome separation to establish proper spindle orientation
title A tetrameric kinesin, Kif25, suppresses centrosome separation to establish proper spindle orientation
title_full A tetrameric kinesin, Kif25, suppresses centrosome separation to establish proper spindle orientation
title_fullStr A tetrameric kinesin, Kif25, suppresses centrosome separation to establish proper spindle orientation
title_full_unstemmed A tetrameric kinesin, Kif25, suppresses centrosome separation to establish proper spindle orientation
title_short A tetrameric kinesin, Kif25, suppresses centrosome separation to establish proper spindle orientation
title_sort tetrameric kinesin, kif25, suppresses centrosome separation to establish proper spindle orientation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376238/
https://www.ncbi.nlm.nih.gov/pubmed/28263957
http://dx.doi.org/10.1038/ncb3486
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