Cargando…
OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese
An increasing number of studies suggest that ornithine decarboxylase antizyme 1 (OAZ1), which is regarded as a tumor suppressor gene, regulates follicular development, ovulation, and steroidogenesis. The granulosa cells in the ovary play a critical role in these ovarian functions. However, the actio...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376318/ https://www.ncbi.nlm.nih.gov/pubmed/28362829 http://dx.doi.org/10.1371/journal.pone.0175016 |
_version_ | 1782519138820816896 |
---|---|
author | Kang, Bo Jiang, Dongmei Ma, Rong He, Hui Yi, Zhixin Chen, Ziyu |
author_facet | Kang, Bo Jiang, Dongmei Ma, Rong He, Hui Yi, Zhixin Chen, Ziyu |
author_sort | Kang, Bo |
collection | PubMed |
description | An increasing number of studies suggest that ornithine decarboxylase antizyme 1 (OAZ1), which is regarded as a tumor suppressor gene, regulates follicular development, ovulation, and steroidogenesis. The granulosa cells in the ovary play a critical role in these ovarian functions. However, the action of OAZ1 mediating physiological functions of granulosa cells is obscure. OAZ1 knockdown in granulosa cells of geese was carried out in the current study. The effect of OAZ1 knockdown on polyamine metabolism, cell proliferation, apoptosis, and hormone receptor transcription of primary granulosa cells in geese was measured. The viability of granulosa cells transfected with the shRNA OAZ1 at 48 h was significantly higher than the control (p<0.05). The level of putrescine and spermidine in granulosa cells down-regulating OAZ1 was 7.04- and 2.11- fold higher compared with the control, respectively (p<0.05). The CCND1, SMAD1, and BCL-2 mRNA expression levels in granulosa cells down-regulating OAZ1 were each significantly higher than the control, respectively (p<0.05), whereas the PCNA and CASPASE 3 expression levels were significantly lower than the control (p<0.05). The estradiol concentration, ER and LHR mRNA expression levels were significantly lower in granulosa cells down-regulating OAZ1 compared with the control (p<0.05). Taken together, our results indicated that OAZ1 knockdown elevated the putrescine and spermidine contents and enhanced granulosa cell viability and inhibited ER and LHR transcriptions of granulosa cells in geese. |
format | Online Article Text |
id | pubmed-5376318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53763182017-04-07 OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese Kang, Bo Jiang, Dongmei Ma, Rong He, Hui Yi, Zhixin Chen, Ziyu PLoS One Research Article An increasing number of studies suggest that ornithine decarboxylase antizyme 1 (OAZ1), which is regarded as a tumor suppressor gene, regulates follicular development, ovulation, and steroidogenesis. The granulosa cells in the ovary play a critical role in these ovarian functions. However, the action of OAZ1 mediating physiological functions of granulosa cells is obscure. OAZ1 knockdown in granulosa cells of geese was carried out in the current study. The effect of OAZ1 knockdown on polyamine metabolism, cell proliferation, apoptosis, and hormone receptor transcription of primary granulosa cells in geese was measured. The viability of granulosa cells transfected with the shRNA OAZ1 at 48 h was significantly higher than the control (p<0.05). The level of putrescine and spermidine in granulosa cells down-regulating OAZ1 was 7.04- and 2.11- fold higher compared with the control, respectively (p<0.05). The CCND1, SMAD1, and BCL-2 mRNA expression levels in granulosa cells down-regulating OAZ1 were each significantly higher than the control, respectively (p<0.05), whereas the PCNA and CASPASE 3 expression levels were significantly lower than the control (p<0.05). The estradiol concentration, ER and LHR mRNA expression levels were significantly lower in granulosa cells down-regulating OAZ1 compared with the control (p<0.05). Taken together, our results indicated that OAZ1 knockdown elevated the putrescine and spermidine contents and enhanced granulosa cell viability and inhibited ER and LHR transcriptions of granulosa cells in geese. Public Library of Science 2017-03-31 /pmc/articles/PMC5376318/ /pubmed/28362829 http://dx.doi.org/10.1371/journal.pone.0175016 Text en © 2017 Kang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kang, Bo Jiang, Dongmei Ma, Rong He, Hui Yi, Zhixin Chen, Ziyu OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese |
title | OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese |
title_full | OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese |
title_fullStr | OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese |
title_full_unstemmed | OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese |
title_short | OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese |
title_sort | oaz1 knockdown enhances viability and inhibits er and lhr transcriptions of granulosa cells in geese |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376318/ https://www.ncbi.nlm.nih.gov/pubmed/28362829 http://dx.doi.org/10.1371/journal.pone.0175016 |
work_keys_str_mv | AT kangbo oaz1knockdownenhancesviabilityandinhibitserandlhrtranscriptionsofgranulosacellsingeese AT jiangdongmei oaz1knockdownenhancesviabilityandinhibitserandlhrtranscriptionsofgranulosacellsingeese AT marong oaz1knockdownenhancesviabilityandinhibitserandlhrtranscriptionsofgranulosacellsingeese AT hehui oaz1knockdownenhancesviabilityandinhibitserandlhrtranscriptionsofgranulosacellsingeese AT yizhixin oaz1knockdownenhancesviabilityandinhibitserandlhrtranscriptionsofgranulosacellsingeese AT chenziyu oaz1knockdownenhancesviabilityandinhibitserandlhrtranscriptionsofgranulosacellsingeese |