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Manipulation of ABA Content in Arabidopsis thaliana Modifies Sensitivity and Oxidative Stress Response to Dickeya dadantii and Influences Peroxidase Activity

The production of reactive oxygen species (ROS) is one of the first defense reactions induced in Arabidopsis in response to infection by the pectinolytic enterobacterium Dickeya dadantii. Previous results also suggest that abscisic acid (ABA) favors D. dadantii multiplication and spread into its hos...

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Autores principales: Van Gijsegem, Frédérique, Pédron, Jacques, Patrit, Oriane, Simond-Côte, Elizabeth, Maia-Grondard, Alessandra, Pétriacq, Pierre, Gonzalez, Raphaël, Blottière, Lydie, Kraepiel, Yvan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376553/
https://www.ncbi.nlm.nih.gov/pubmed/28421092
http://dx.doi.org/10.3389/fpls.2017.00456
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author Van Gijsegem, Frédérique
Pédron, Jacques
Patrit, Oriane
Simond-Côte, Elizabeth
Maia-Grondard, Alessandra
Pétriacq, Pierre
Gonzalez, Raphaël
Blottière, Lydie
Kraepiel, Yvan
author_facet Van Gijsegem, Frédérique
Pédron, Jacques
Patrit, Oriane
Simond-Côte, Elizabeth
Maia-Grondard, Alessandra
Pétriacq, Pierre
Gonzalez, Raphaël
Blottière, Lydie
Kraepiel, Yvan
author_sort Van Gijsegem, Frédérique
collection PubMed
description The production of reactive oxygen species (ROS) is one of the first defense reactions induced in Arabidopsis in response to infection by the pectinolytic enterobacterium Dickeya dadantii. Previous results also suggest that abscisic acid (ABA) favors D. dadantii multiplication and spread into its hosts. Here, we confirm this hypothesis using ABA-deficient and ABA-overproducer Arabidopsis plants. We investigated the relationships between ABA status and ROS production in Arabidopsis after D. dadantii infection and showed that ABA status modulates the capacity of the plant to produce ROS in response to infection by decreasing the production of class III peroxidases. This mechanism takes place independently of the well-described oxidative stress related to the RBOHD NADPH oxidase. In addition to this weakening of plant defense, ABA content in the plant correlates positively with the production of some bacterial virulence factors during the first stages of infection. Both processes should enhance disease progression in presence of high ABA content. Given that infection increases transcript abundance for the ABA biosynthesis genes AAO3 and ABA3 and triggers ABA accumulation in leaves, we propose that D. dadantii manipulates ABA homeostasis as part of its virulence strategy.
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spelling pubmed-53765532017-04-18 Manipulation of ABA Content in Arabidopsis thaliana Modifies Sensitivity and Oxidative Stress Response to Dickeya dadantii and Influences Peroxidase Activity Van Gijsegem, Frédérique Pédron, Jacques Patrit, Oriane Simond-Côte, Elizabeth Maia-Grondard, Alessandra Pétriacq, Pierre Gonzalez, Raphaël Blottière, Lydie Kraepiel, Yvan Front Plant Sci Plant Science The production of reactive oxygen species (ROS) is one of the first defense reactions induced in Arabidopsis in response to infection by the pectinolytic enterobacterium Dickeya dadantii. Previous results also suggest that abscisic acid (ABA) favors D. dadantii multiplication and spread into its hosts. Here, we confirm this hypothesis using ABA-deficient and ABA-overproducer Arabidopsis plants. We investigated the relationships between ABA status and ROS production in Arabidopsis after D. dadantii infection and showed that ABA status modulates the capacity of the plant to produce ROS in response to infection by decreasing the production of class III peroxidases. This mechanism takes place independently of the well-described oxidative stress related to the RBOHD NADPH oxidase. In addition to this weakening of plant defense, ABA content in the plant correlates positively with the production of some bacterial virulence factors during the first stages of infection. Both processes should enhance disease progression in presence of high ABA content. Given that infection increases transcript abundance for the ABA biosynthesis genes AAO3 and ABA3 and triggers ABA accumulation in leaves, we propose that D. dadantii manipulates ABA homeostasis as part of its virulence strategy. Frontiers Media S.A. 2017-04-03 /pmc/articles/PMC5376553/ /pubmed/28421092 http://dx.doi.org/10.3389/fpls.2017.00456 Text en Copyright © 2017 Van Gijsegem, Pédron, Patrit, Simond-Côte, Maia-Grondard, Pétriacq, Gonzalez, Blottière and Kraepiel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Van Gijsegem, Frédérique
Pédron, Jacques
Patrit, Oriane
Simond-Côte, Elizabeth
Maia-Grondard, Alessandra
Pétriacq, Pierre
Gonzalez, Raphaël
Blottière, Lydie
Kraepiel, Yvan
Manipulation of ABA Content in Arabidopsis thaliana Modifies Sensitivity and Oxidative Stress Response to Dickeya dadantii and Influences Peroxidase Activity
title Manipulation of ABA Content in Arabidopsis thaliana Modifies Sensitivity and Oxidative Stress Response to Dickeya dadantii and Influences Peroxidase Activity
title_full Manipulation of ABA Content in Arabidopsis thaliana Modifies Sensitivity and Oxidative Stress Response to Dickeya dadantii and Influences Peroxidase Activity
title_fullStr Manipulation of ABA Content in Arabidopsis thaliana Modifies Sensitivity and Oxidative Stress Response to Dickeya dadantii and Influences Peroxidase Activity
title_full_unstemmed Manipulation of ABA Content in Arabidopsis thaliana Modifies Sensitivity and Oxidative Stress Response to Dickeya dadantii and Influences Peroxidase Activity
title_short Manipulation of ABA Content in Arabidopsis thaliana Modifies Sensitivity and Oxidative Stress Response to Dickeya dadantii and Influences Peroxidase Activity
title_sort manipulation of aba content in arabidopsis thaliana modifies sensitivity and oxidative stress response to dickeya dadantii and influences peroxidase activity
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376553/
https://www.ncbi.nlm.nih.gov/pubmed/28421092
http://dx.doi.org/10.3389/fpls.2017.00456
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