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Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing

The gut ecosystem is characterized by dynamic and reciprocal interactions between the host and bacteria. Although characterizing microbiota for herbivores has become recognized as important tool for gauging species health, no study to date has investigated the bacterial communities and evaluated the...

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Autores principales: Hu, Xiaolong, Liu, Gang, Shafer, Aaron B. A., Wei, Yuting, Zhou, Juntong, Lin, Shaobi, Wu, Haibin, Zhou, Mi, Hu, Defu, Liu, Shuqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376572/
https://www.ncbi.nlm.nih.gov/pubmed/28421061
http://dx.doi.org/10.3389/fmicb.2017.00572
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author Hu, Xiaolong
Liu, Gang
Shafer, Aaron B. A.
Wei, Yuting
Zhou, Juntong
Lin, Shaobi
Wu, Haibin
Zhou, Mi
Hu, Defu
Liu, Shuqiang
author_facet Hu, Xiaolong
Liu, Gang
Shafer, Aaron B. A.
Wei, Yuting
Zhou, Juntong
Lin, Shaobi
Wu, Haibin
Zhou, Mi
Hu, Defu
Liu, Shuqiang
author_sort Hu, Xiaolong
collection PubMed
description The gut ecosystem is characterized by dynamic and reciprocal interactions between the host and bacteria. Although characterizing microbiota for herbivores has become recognized as important tool for gauging species health, no study to date has investigated the bacterial communities and evaluated the age-related bacterial dynamics of musk deer. Moreover, gastrointestinal diseases have been hypothesized to be a limiting factor of population growth in captive musk deer. Here, high-throughput sequencing of the bacterial 16S rRNA gene was used to profile the fecal bacterial communities in juvenile and adult alpine and forest musk deer. The two musk deer species harbored similar bacterial communities at the phylum level, whereas the key genera for the two species were distinct. The bacterial communities were dominated by Firmicutes and Bacteroidetes, with the bacterial diversity being higher in forest musk deer. The Firmicutes to Bacteroidetes ratio also increased from juvenile to adult, while the bacterial diversity, within-group and between-group similarity, all increased with age. This work serves as the first sequence-based analysis of variation in bacterial communities within and between musk deer species, and demonstrates how the gut microbial community dynamics vary among closely related species and shift with age. As gastrointestinal diseases have been observed in captive populations, this study provides valuable data that might benefit captive management and future reintroduction programs.
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spelling pubmed-53765722017-04-18 Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing Hu, Xiaolong Liu, Gang Shafer, Aaron B. A. Wei, Yuting Zhou, Juntong Lin, Shaobi Wu, Haibin Zhou, Mi Hu, Defu Liu, Shuqiang Front Microbiol Microbiology The gut ecosystem is characterized by dynamic and reciprocal interactions between the host and bacteria. Although characterizing microbiota for herbivores has become recognized as important tool for gauging species health, no study to date has investigated the bacterial communities and evaluated the age-related bacterial dynamics of musk deer. Moreover, gastrointestinal diseases have been hypothesized to be a limiting factor of population growth in captive musk deer. Here, high-throughput sequencing of the bacterial 16S rRNA gene was used to profile the fecal bacterial communities in juvenile and adult alpine and forest musk deer. The two musk deer species harbored similar bacterial communities at the phylum level, whereas the key genera for the two species were distinct. The bacterial communities were dominated by Firmicutes and Bacteroidetes, with the bacterial diversity being higher in forest musk deer. The Firmicutes to Bacteroidetes ratio also increased from juvenile to adult, while the bacterial diversity, within-group and between-group similarity, all increased with age. This work serves as the first sequence-based analysis of variation in bacterial communities within and between musk deer species, and demonstrates how the gut microbial community dynamics vary among closely related species and shift with age. As gastrointestinal diseases have been observed in captive populations, this study provides valuable data that might benefit captive management and future reintroduction programs. Frontiers Media S.A. 2017-04-03 /pmc/articles/PMC5376572/ /pubmed/28421061 http://dx.doi.org/10.3389/fmicb.2017.00572 Text en Copyright © 2017 Hu, Liu, Shafer, Wei, Zhou, Lin, Wu, Zhou, Hu and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hu, Xiaolong
Liu, Gang
Shafer, Aaron B. A.
Wei, Yuting
Zhou, Juntong
Lin, Shaobi
Wu, Haibin
Zhou, Mi
Hu, Defu
Liu, Shuqiang
Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing
title Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing
title_full Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing
title_fullStr Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing
title_full_unstemmed Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing
title_short Comparative Analysis of the Gut Microbial Communities in Forest and Alpine Musk Deer Using High-Throughput Sequencing
title_sort comparative analysis of the gut microbial communities in forest and alpine musk deer using high-throughput sequencing
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376572/
https://www.ncbi.nlm.nih.gov/pubmed/28421061
http://dx.doi.org/10.3389/fmicb.2017.00572
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