A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

BACKGROUND: Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many instances these insertional mutants have helped elucidate the genetic basis of v...

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Detalles Bibliográficos
Autores principales: Pollock, Steve V., Mukherjee, Bratati, Bajsa-Hirschel, Joanna, Machingura, Marylou C., Mukherjee, Ananya, Grossman, Arthur R., Moroney, James V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376698/
https://www.ncbi.nlm.nih.gov/pubmed/28392829
http://dx.doi.org/10.1186/s13007-017-0170-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376698/
https://www.ncbi.nlm.nih.gov/pubmed/28392829
http://dx.doi.org/10.1186/s13007-017-0170-x

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