Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions

Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAX...

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Detalles Bibliográficos
Autores principales: Graewert, Melissa A., Franke, Daniel, Jeffries, Cy M., Blanchet, Clement E., Ruskule, Darja, Kuhle, Katja, Flieger, Antje, Schäfer, Bernd, Tartsch, Bernd, Meijers, Rob, Svergun, Dmitri I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377070/
https://www.ncbi.nlm.nih.gov/pubmed/26030009
http://dx.doi.org/10.1038/srep10734
Descripción
Sumario:Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).

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