Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions

Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAX...

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Autores principales: Graewert, Melissa A., Franke, Daniel, Jeffries, Cy M., Blanchet, Clement E., Ruskule, Darja, Kuhle, Katja, Flieger, Antje, Schäfer, Bernd, Tartsch, Bernd, Meijers, Rob, Svergun, Dmitri I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377070/
https://www.ncbi.nlm.nih.gov/pubmed/26030009
http://dx.doi.org/10.1038/srep10734
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author Graewert, Melissa A.
Franke, Daniel
Jeffries, Cy M.
Blanchet, Clement E.
Ruskule, Darja
Kuhle, Katja
Flieger, Antje
Schäfer, Bernd
Tartsch, Bernd
Meijers, Rob
Svergun, Dmitri I.
author_facet Graewert, Melissa A.
Franke, Daniel
Jeffries, Cy M.
Blanchet, Clement E.
Ruskule, Darja
Kuhle, Katja
Flieger, Antje
Schäfer, Bernd
Tartsch, Bernd
Meijers, Rob
Svergun, Dmitri I.
author_sort Graewert, Melissa A.
collection PubMed
description Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).
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spelling pubmed-53770702017-04-07 Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions Graewert, Melissa A. Franke, Daniel Jeffries, Cy M. Blanchet, Clement E. Ruskule, Darja Kuhle, Katja Flieger, Antje Schäfer, Bernd Tartsch, Bernd Meijers, Rob Svergun, Dmitri I. Sci Rep Article Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg). Nature Publishing Group 2015-06-01 /pmc/articles/PMC5377070/ /pubmed/26030009 http://dx.doi.org/10.1038/srep10734 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Graewert, Melissa A.
Franke, Daniel
Jeffries, Cy M.
Blanchet, Clement E.
Ruskule, Darja
Kuhle, Katja
Flieger, Antje
Schäfer, Bernd
Tartsch, Bernd
Meijers, Rob
Svergun, Dmitri I.
Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
title Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
title_full Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
title_fullStr Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
title_full_unstemmed Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
title_short Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
title_sort automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377070/
https://www.ncbi.nlm.nih.gov/pubmed/26030009
http://dx.doi.org/10.1038/srep10734
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