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Myo-inositol improves the host’s ability to eliminate balofloxacin-resistant Escherichia coli

Antibiotic-resistant mechanisms are associated with fitness costs. However, why antibiotic-resistant bacteria usually show increasing adaptation to hosts is largely unknown, especially from the host’s perspective. The present study reveals the host’s varied response to balofloxacin-resistant Escheri...

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Autores principales: Chen, Xin-hai, Zhang, Bing-wen, Li, Hui, Peng, Xuan-xian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377236/
https://www.ncbi.nlm.nih.gov/pubmed/26030712
http://dx.doi.org/10.1038/srep10720
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author Chen, Xin-hai
Zhang, Bing-wen
Li, Hui
Peng, Xuan-xian
author_facet Chen, Xin-hai
Zhang, Bing-wen
Li, Hui
Peng, Xuan-xian
author_sort Chen, Xin-hai
collection PubMed
description Antibiotic-resistant mechanisms are associated with fitness costs. However, why antibiotic-resistant bacteria usually show increasing adaptation to hosts is largely unknown, especially from the host’s perspective. The present study reveals the host’s varied response to balofloxacin-resistant Escherichia coli (BLFX-R) using an integrated proteome and metabolome approach and identifies myo-inositol and phagocytosis-related proteins as crucial biomarkers. Originally, macrophages have an optimal attractive preference to BLFX-S due to more polarization of BLFX-S than BLFX-R, which renders faster elimination to BLFX-S than BLFX-R. The slower elimination to BLFX-R may be reversed by exogenous myo-inositol. Primarily, myo-inositol depolarizes macrophages, elevating adherence to both BLFX-S and BLFX-R. Since the altered adherence is equal to both strains, the myo-inositol-treated macrophages are free of the barrier to BLFX-R and thereby promote phagocytosis of BLFX-R. This work provides a novel strategy based on metabolic modulation for eliminating antibiotic-resistant bacteria with a high degree of host adaptation.
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spelling pubmed-53772362017-04-07 Myo-inositol improves the host’s ability to eliminate balofloxacin-resistant Escherichia coli Chen, Xin-hai Zhang, Bing-wen Li, Hui Peng, Xuan-xian Sci Rep Article Antibiotic-resistant mechanisms are associated with fitness costs. However, why antibiotic-resistant bacteria usually show increasing adaptation to hosts is largely unknown, especially from the host’s perspective. The present study reveals the host’s varied response to balofloxacin-resistant Escherichia coli (BLFX-R) using an integrated proteome and metabolome approach and identifies myo-inositol and phagocytosis-related proteins as crucial biomarkers. Originally, macrophages have an optimal attractive preference to BLFX-S due to more polarization of BLFX-S than BLFX-R, which renders faster elimination to BLFX-S than BLFX-R. The slower elimination to BLFX-R may be reversed by exogenous myo-inositol. Primarily, myo-inositol depolarizes macrophages, elevating adherence to both BLFX-S and BLFX-R. Since the altered adherence is equal to both strains, the myo-inositol-treated macrophages are free of the barrier to BLFX-R and thereby promote phagocytosis of BLFX-R. This work provides a novel strategy based on metabolic modulation for eliminating antibiotic-resistant bacteria with a high degree of host adaptation. Nature Publishing Group 2015-06-01 /pmc/articles/PMC5377236/ /pubmed/26030712 http://dx.doi.org/10.1038/srep10720 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chen, Xin-hai
Zhang, Bing-wen
Li, Hui
Peng, Xuan-xian
Myo-inositol improves the host’s ability to eliminate balofloxacin-resistant Escherichia coli
title Myo-inositol improves the host’s ability to eliminate balofloxacin-resistant Escherichia coli
title_full Myo-inositol improves the host’s ability to eliminate balofloxacin-resistant Escherichia coli
title_fullStr Myo-inositol improves the host’s ability to eliminate balofloxacin-resistant Escherichia coli
title_full_unstemmed Myo-inositol improves the host’s ability to eliminate balofloxacin-resistant Escherichia coli
title_short Myo-inositol improves the host’s ability to eliminate balofloxacin-resistant Escherichia coli
title_sort myo-inositol improves the host’s ability to eliminate balofloxacin-resistant escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377236/
https://www.ncbi.nlm.nih.gov/pubmed/26030712
http://dx.doi.org/10.1038/srep10720
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