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Genetic and Proteomic characterization of Bile Salt Export Pump (BSEP) in Snake Liver

Snake gallbladder, a traditional Chinese medicine, has been believed in various Asian countries to improve visual acuity and alleviate rheumatism. Bile acids, a major component of the gallbladder, are toxic to the liver and kidney in humans and animals due to its detergent effects, while also exhibi...

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Autores principales: Tan, Xinle, Gao, Fei, Su, Hexiu, Gong, Yajun, Zhang, Jie, Sullivan, Mitchell A., Chen, Jiachun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377248/
https://www.ncbi.nlm.nih.gov/pubmed/28368001
http://dx.doi.org/10.1038/srep43556
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author Tan, Xinle
Gao, Fei
Su, Hexiu
Gong, Yajun
Zhang, Jie
Sullivan, Mitchell A.
Chen, Jiachun
author_facet Tan, Xinle
Gao, Fei
Su, Hexiu
Gong, Yajun
Zhang, Jie
Sullivan, Mitchell A.
Chen, Jiachun
author_sort Tan, Xinle
collection PubMed
description Snake gallbladder, a traditional Chinese medicine, has been believed in various Asian countries to improve visual acuity and alleviate rheumatism. Bile acids, a major component of the gallbladder, are toxic to the liver and kidney in humans and animals due to its detergent effects, while also exhibiting therapeutic effects due to an increase in the gallbladder contractions of muscle strips in patients with cholesterol gallstones. Secretion of bile acids in human and mammals depends on the bile salt export pump (BSEP), a liver-specific adenosine triphosphate (ATP)-binding cassette transporter encoded by ABCB11. However, the presence of BSEP in snakes has not been thoroughly explored. Here we confirm the existence of BSEP and its coding DNA sequence in snakes on both the proteomic and genetic level. This work provides information on the snake ABCB11 sequence and helps further potential genetic manipulation to affect bile salt metabolism. Our study provides the foundation for research on bile acid production from snakes by using modern genetic and proteomic methodologies.
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spelling pubmed-53772482017-04-10 Genetic and Proteomic characterization of Bile Salt Export Pump (BSEP) in Snake Liver Tan, Xinle Gao, Fei Su, Hexiu Gong, Yajun Zhang, Jie Sullivan, Mitchell A. Chen, Jiachun Sci Rep Article Snake gallbladder, a traditional Chinese medicine, has been believed in various Asian countries to improve visual acuity and alleviate rheumatism. Bile acids, a major component of the gallbladder, are toxic to the liver and kidney in humans and animals due to its detergent effects, while also exhibiting therapeutic effects due to an increase in the gallbladder contractions of muscle strips in patients with cholesterol gallstones. Secretion of bile acids in human and mammals depends on the bile salt export pump (BSEP), a liver-specific adenosine triphosphate (ATP)-binding cassette transporter encoded by ABCB11. However, the presence of BSEP in snakes has not been thoroughly explored. Here we confirm the existence of BSEP and its coding DNA sequence in snakes on both the proteomic and genetic level. This work provides information on the snake ABCB11 sequence and helps further potential genetic manipulation to affect bile salt metabolism. Our study provides the foundation for research on bile acid production from snakes by using modern genetic and proteomic methodologies. Nature Publishing Group 2017-04-03 /pmc/articles/PMC5377248/ /pubmed/28368001 http://dx.doi.org/10.1038/srep43556 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tan, Xinle
Gao, Fei
Su, Hexiu
Gong, Yajun
Zhang, Jie
Sullivan, Mitchell A.
Chen, Jiachun
Genetic and Proteomic characterization of Bile Salt Export Pump (BSEP) in Snake Liver
title Genetic and Proteomic characterization of Bile Salt Export Pump (BSEP) in Snake Liver
title_full Genetic and Proteomic characterization of Bile Salt Export Pump (BSEP) in Snake Liver
title_fullStr Genetic and Proteomic characterization of Bile Salt Export Pump (BSEP) in Snake Liver
title_full_unstemmed Genetic and Proteomic characterization of Bile Salt Export Pump (BSEP) in Snake Liver
title_short Genetic and Proteomic characterization of Bile Salt Export Pump (BSEP) in Snake Liver
title_sort genetic and proteomic characterization of bile salt export pump (bsep) in snake liver
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377248/
https://www.ncbi.nlm.nih.gov/pubmed/28368001
http://dx.doi.org/10.1038/srep43556
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