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Controlling T-Cell Activation with Synthetic Dendritic Cells Using the Multivalency Effect
[Image: see text] Artificial antigen-presenting cells (aAPCs) have recently gained a lot of attention. They efficiently activate T cells and serve as powerful replacements for dendritic cells in cancer immunotherapy. Focusing on a specific class of polymer-based aAPCs, so-called synthetic dendritic...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377267/ https://www.ncbi.nlm.nih.gov/pubmed/28393131 http://dx.doi.org/10.1021/acsomega.6b00436 |
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author | Hammink, Roel Mandal, Subhra Eggermont, Loek J. Nooteboom, Marco Willems, Peter H. G. M. Tel, Jurjen Rowan, Alan E. Figdor, Carl G. Blank, Kerstin G. |
author_facet | Hammink, Roel Mandal, Subhra Eggermont, Loek J. Nooteboom, Marco Willems, Peter H. G. M. Tel, Jurjen Rowan, Alan E. Figdor, Carl G. Blank, Kerstin G. |
author_sort | Hammink, Roel |
collection | PubMed |
description | [Image: see text] Artificial antigen-presenting cells (aAPCs) have recently gained a lot of attention. They efficiently activate T cells and serve as powerful replacements for dendritic cells in cancer immunotherapy. Focusing on a specific class of polymer-based aAPCs, so-called synthetic dendritic cells (sDCs), we have investigated the importance of multivalent binding on T-cell activation. Using antibody-functionalized sDCs, we have tested the influence of polymer length and antibody density. Increasing the multivalent character of the antibody-functionalized polymer lowered the effective concentration required for T-cell activation. This was evidenced for both early and late stages of activation. The most important effect observed was the significantly prolonged activation of the stimulated T cells, indicating that multivalent sDCs sustain T-cell signaling. Our results highlight the importance of multivalency for the design of aAPCs and will ultimately allow for better mimics of natural dendritic cells that can be used as vaccines in cancer treatment. |
format | Online Article Text |
id | pubmed-5377267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-53772672017-04-05 Controlling T-Cell Activation with Synthetic Dendritic Cells Using the Multivalency Effect Hammink, Roel Mandal, Subhra Eggermont, Loek J. Nooteboom, Marco Willems, Peter H. G. M. Tel, Jurjen Rowan, Alan E. Figdor, Carl G. Blank, Kerstin G. ACS Omega [Image: see text] Artificial antigen-presenting cells (aAPCs) have recently gained a lot of attention. They efficiently activate T cells and serve as powerful replacements for dendritic cells in cancer immunotherapy. Focusing on a specific class of polymer-based aAPCs, so-called synthetic dendritic cells (sDCs), we have investigated the importance of multivalent binding on T-cell activation. Using antibody-functionalized sDCs, we have tested the influence of polymer length and antibody density. Increasing the multivalent character of the antibody-functionalized polymer lowered the effective concentration required for T-cell activation. This was evidenced for both early and late stages of activation. The most important effect observed was the significantly prolonged activation of the stimulated T cells, indicating that multivalent sDCs sustain T-cell signaling. Our results highlight the importance of multivalency for the design of aAPCs and will ultimately allow for better mimics of natural dendritic cells that can be used as vaccines in cancer treatment. American Chemical Society 2017-03-16 /pmc/articles/PMC5377267/ /pubmed/28393131 http://dx.doi.org/10.1021/acsomega.6b00436 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Hammink, Roel Mandal, Subhra Eggermont, Loek J. Nooteboom, Marco Willems, Peter H. G. M. Tel, Jurjen Rowan, Alan E. Figdor, Carl G. Blank, Kerstin G. Controlling T-Cell Activation with Synthetic Dendritic Cells Using the Multivalency Effect |
title | Controlling T-Cell Activation with Synthetic
Dendritic Cells Using the Multivalency Effect |
title_full | Controlling T-Cell Activation with Synthetic
Dendritic Cells Using the Multivalency Effect |
title_fullStr | Controlling T-Cell Activation with Synthetic
Dendritic Cells Using the Multivalency Effect |
title_full_unstemmed | Controlling T-Cell Activation with Synthetic
Dendritic Cells Using the Multivalency Effect |
title_short | Controlling T-Cell Activation with Synthetic
Dendritic Cells Using the Multivalency Effect |
title_sort | controlling t-cell activation with synthetic
dendritic cells using the multivalency effect |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377267/ https://www.ncbi.nlm.nih.gov/pubmed/28393131 http://dx.doi.org/10.1021/acsomega.6b00436 |
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