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MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells

The aim of the present study was to evaluate the functions of miR-200c in the regulation of tumor growth and metastasis in renal cancer cells, and to investigate the underlying mechanisms. In this study, miR-200c was up- and downregulated in two renal cancer cell lines, namely ACHN and A498, and the...

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Autores principales: Qiu, Mingning, Liang, Ziji, Chen, Lieqian, Tan, Guobin, Liu, Lei, Wang, Kangning, Chen, Hege, Liu, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377423/
https://www.ncbi.nlm.nih.gov/pubmed/28413473
http://dx.doi.org/10.3892/etm.2017.4147
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author Qiu, Mingning
Liang, Ziji
Chen, Lieqian
Tan, Guobin
Liu, Lei
Wang, Kangning
Chen, Hege
Liu, Jianjun
author_facet Qiu, Mingning
Liang, Ziji
Chen, Lieqian
Tan, Guobin
Liu, Lei
Wang, Kangning
Chen, Hege
Liu, Jianjun
author_sort Qiu, Mingning
collection PubMed
description The aim of the present study was to evaluate the functions of miR-200c in the regulation of tumor growth and metastasis in renal cancer cells, and to investigate the underlying mechanisms. In this study, miR-200c was up- and downregulated in two renal cancer cell lines, namely ACHN and A498, and the proliferation, colony formation, migration and invasion of the cells were measured. The expression levels of various mRNAs and proteins were then analyzed using reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. It was found that miR-200c suppressed proliferation, migration and invasion of the renal cancer cells and, conversely, the inhibition of endogenous miR-200c resulted in increased cell proliferation and metastasis. Furthermore, a luciferase reporter assay revealed that miR-200c directly targeted the 3′ untranslated regions of the oncogenes B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi-1) and E2F transcription factor 3 (E2F3) mRNAs, reduced the expression of Bmi-1 and E2F3 and regulated the expression of downstream genes, including E-cadherin, N-cadherin, vimentin, p14 and p16. These results indicate a tumor suppressor role for miR-200c in renal cancer cells via the direct targeting of Bmi-1 and E2F3.
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spelling pubmed-53774232017-04-15 MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells Qiu, Mingning Liang, Ziji Chen, Lieqian Tan, Guobin Liu, Lei Wang, Kangning Chen, Hege Liu, Jianjun Exp Ther Med Articles The aim of the present study was to evaluate the functions of miR-200c in the regulation of tumor growth and metastasis in renal cancer cells, and to investigate the underlying mechanisms. In this study, miR-200c was up- and downregulated in two renal cancer cell lines, namely ACHN and A498, and the proliferation, colony formation, migration and invasion of the cells were measured. The expression levels of various mRNAs and proteins were then analyzed using reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. It was found that miR-200c suppressed proliferation, migration and invasion of the renal cancer cells and, conversely, the inhibition of endogenous miR-200c resulted in increased cell proliferation and metastasis. Furthermore, a luciferase reporter assay revealed that miR-200c directly targeted the 3′ untranslated regions of the oncogenes B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi-1) and E2F transcription factor 3 (E2F3) mRNAs, reduced the expression of Bmi-1 and E2F3 and regulated the expression of downstream genes, including E-cadherin, N-cadherin, vimentin, p14 and p16. These results indicate a tumor suppressor role for miR-200c in renal cancer cells via the direct targeting of Bmi-1 and E2F3. D.A. Spandidos 2017-04 2017-02-21 /pmc/articles/PMC5377423/ /pubmed/28413473 http://dx.doi.org/10.3892/etm.2017.4147 Text en Copyright: © Qiu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Qiu, Mingning
Liang, Ziji
Chen, Lieqian
Tan, Guobin
Liu, Lei
Wang, Kangning
Chen, Hege
Liu, Jianjun
MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells
title MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells
title_full MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells
title_fullStr MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells
title_full_unstemmed MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells
title_short MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells
title_sort microrna-200c suppresses cell growth and metastasis by targeting bmi-1 and e2f3 in renal cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377423/
https://www.ncbi.nlm.nih.gov/pubmed/28413473
http://dx.doi.org/10.3892/etm.2017.4147
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