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Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran

BACKGROUND: The incidence rate of breast cancer has been dramatically increasing since the last decade in Iran, and it is now one of the most common female malignant tumors. B-cell lymphoma 2 (BCL2) family is the most important regulator of apoptosis, and −938C>A single nucleotide polymorphism (S...

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Autores principales: Moghaddam, Sepideh Esfahani, Barzegar, Ali, Nikbakhsh, Novin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377969/
https://www.ncbi.nlm.nih.gov/pubmed/28413418
http://dx.doi.org/10.4103/1735-1995.200269
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author Moghaddam, Sepideh Esfahani
Barzegar, Ali
Nikbakhsh, Novin
author_facet Moghaddam, Sepideh Esfahani
Barzegar, Ali
Nikbakhsh, Novin
author_sort Moghaddam, Sepideh Esfahani
collection PubMed
description BACKGROUND: The incidence rate of breast cancer has been dramatically increasing since the last decade in Iran, and it is now one of the most common female malignant tumors. B-cell lymphoma 2 (BCL2) family is the most important regulator of apoptosis, and −938C>A single nucleotide polymorphism (SNP) of BCL2 gene promoter has been demonstrated to influence breast cancer susceptibility. In this research, we study the effect of −938C>A allelic variants on breast cancer risk in Mazandaran province at the North of Iran. MATERIALS AND METHODS: This analysis performed on 120 breast cancer patients who underwent surgery in some referenced hospitals at Mazandaran province along with 130 healthy individuals as a control. DNA extracted from peripheral blood samples was applied in polymerase chain reaction-single-strand conformation polymorphism analysis to determine −938C>A genotype. The association of the −938C>A genotype and breast cancer risk as well as clinicopathological characters were analyzed by logistic regression method. RESULTS: Results showed that genotype frequency of AA, AC, and CC genotypes was 10%, 62%, and 28% for case and 28%, 50%, and 22% in control group, respectively. In the logistic regression model, BCL2 − 938C/A variant genotype AA was associated with a decreased risk of breast cancer (P = 0.041) by 0.31-fold (odds ratio = 0.31, confidence interval = 0.091–0.909) compared to CC genotype. However, no significant association found between −938C>A genotype and clinicopathological characters. CONCLUSION: The study showed that AA genotype of BCL2 gene (−938C>A) is associated with decreased susceptibility to breast cancer. Hence, investigating the −938C>A SNP of BCL2 gene promoter could be an appropriate molecular marker to determine individual sensitivity to breast cancer.
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spelling pubmed-53779692017-04-14 Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran Moghaddam, Sepideh Esfahani Barzegar, Ali Nikbakhsh, Novin J Res Med Sci Original Article BACKGROUND: The incidence rate of breast cancer has been dramatically increasing since the last decade in Iran, and it is now one of the most common female malignant tumors. B-cell lymphoma 2 (BCL2) family is the most important regulator of apoptosis, and −938C>A single nucleotide polymorphism (SNP) of BCL2 gene promoter has been demonstrated to influence breast cancer susceptibility. In this research, we study the effect of −938C>A allelic variants on breast cancer risk in Mazandaran province at the North of Iran. MATERIALS AND METHODS: This analysis performed on 120 breast cancer patients who underwent surgery in some referenced hospitals at Mazandaran province along with 130 healthy individuals as a control. DNA extracted from peripheral blood samples was applied in polymerase chain reaction-single-strand conformation polymorphism analysis to determine −938C>A genotype. The association of the −938C>A genotype and breast cancer risk as well as clinicopathological characters were analyzed by logistic regression method. RESULTS: Results showed that genotype frequency of AA, AC, and CC genotypes was 10%, 62%, and 28% for case and 28%, 50%, and 22% in control group, respectively. In the logistic regression model, BCL2 − 938C/A variant genotype AA was associated with a decreased risk of breast cancer (P = 0.041) by 0.31-fold (odds ratio = 0.31, confidence interval = 0.091–0.909) compared to CC genotype. However, no significant association found between −938C>A genotype and clinicopathological characters. CONCLUSION: The study showed that AA genotype of BCL2 gene (−938C>A) is associated with decreased susceptibility to breast cancer. Hence, investigating the −938C>A SNP of BCL2 gene promoter could be an appropriate molecular marker to determine individual sensitivity to breast cancer. Medknow Publications & Media Pvt Ltd 2017-02-16 /pmc/articles/PMC5377969/ /pubmed/28413418 http://dx.doi.org/10.4103/1735-1995.200269 Text en Copyright: © 2017 Journal of Research in Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Moghaddam, Sepideh Esfahani
Barzegar, Ali
Nikbakhsh, Novin
Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran
title Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran
title_full Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran
title_fullStr Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran
title_full_unstemmed Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran
title_short Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran
title_sort study of the regulatory promoter polymorphism (−938c>a) of b-cell lymphoma 2 gene in breast cancer patients of mazandaran province in northern iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377969/
https://www.ncbi.nlm.nih.gov/pubmed/28413418
http://dx.doi.org/10.4103/1735-1995.200269
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