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Cholesterolomics: An update

Cholesterolomics can be regarded as the identification and quantification of cholesterol, its precursors post squalene, and metabolites of cholesterol and of its precursors, in a biological sample. These molecules include 1,25-dihydroxyvitamin D(3), steroid hormones and bile acids and intermediates...

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Detalles Bibliográficos
Autores principales: Griffiths, William J., Abdel-Khalik, Jonas, Yutuc, Eylan, Morgan, Alwena H., Gilmore, Ian, Hearn, Thomas, Wang, Yuqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378159/
https://www.ncbi.nlm.nih.gov/pubmed/28087213
http://dx.doi.org/10.1016/j.ab.2017.01.009
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author Griffiths, William J.
Abdel-Khalik, Jonas
Yutuc, Eylan
Morgan, Alwena H.
Gilmore, Ian
Hearn, Thomas
Wang, Yuqin
author_facet Griffiths, William J.
Abdel-Khalik, Jonas
Yutuc, Eylan
Morgan, Alwena H.
Gilmore, Ian
Hearn, Thomas
Wang, Yuqin
author_sort Griffiths, William J.
collection PubMed
description Cholesterolomics can be regarded as the identification and quantification of cholesterol, its precursors post squalene, and metabolites of cholesterol and of its precursors, in a biological sample. These molecules include 1,25-dihydroxyvitamin D(3), steroid hormones and bile acids and intermediates in their respective biosynthetic pathways. In this short article we will concentrate our attention on intermediates in bile acid biosynthesis pathways, in particular oxysterols and cholestenoic acids. These molecular classes are implicated in the aetiology of a diverse array of diseases including autoimmune disease, Parkinson's disease, motor neuron disease, breast cancer, the lysosomal storage disease Niemann-Pick type C and the autosomal recessive disorder Smith-Lemli-Opitz syndrome. Mass spectrometry (MS) is the dominant technology for sterol analysis including both gas-chromatography (GC)-MS and liquid chromatography (LC)-MS and more recently matrix-assisted laser desorption/ionisation (MALDI)-MS for tissue imaging studies. Here we will discuss exciting biological findings and recent analytical improvements.
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spelling pubmed-53781592017-05-01 Cholesterolomics: An update Griffiths, William J. Abdel-Khalik, Jonas Yutuc, Eylan Morgan, Alwena H. Gilmore, Ian Hearn, Thomas Wang, Yuqin Anal Biochem Article Cholesterolomics can be regarded as the identification and quantification of cholesterol, its precursors post squalene, and metabolites of cholesterol and of its precursors, in a biological sample. These molecules include 1,25-dihydroxyvitamin D(3), steroid hormones and bile acids and intermediates in their respective biosynthetic pathways. In this short article we will concentrate our attention on intermediates in bile acid biosynthesis pathways, in particular oxysterols and cholestenoic acids. These molecular classes are implicated in the aetiology of a diverse array of diseases including autoimmune disease, Parkinson's disease, motor neuron disease, breast cancer, the lysosomal storage disease Niemann-Pick type C and the autosomal recessive disorder Smith-Lemli-Opitz syndrome. Mass spectrometry (MS) is the dominant technology for sterol analysis including both gas-chromatography (GC)-MS and liquid chromatography (LC)-MS and more recently matrix-assisted laser desorption/ionisation (MALDI)-MS for tissue imaging studies. Here we will discuss exciting biological findings and recent analytical improvements. Elsevier 2017-05-01 /pmc/articles/PMC5378159/ /pubmed/28087213 http://dx.doi.org/10.1016/j.ab.2017.01.009 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Griffiths, William J.
Abdel-Khalik, Jonas
Yutuc, Eylan
Morgan, Alwena H.
Gilmore, Ian
Hearn, Thomas
Wang, Yuqin
Cholesterolomics: An update
title Cholesterolomics: An update
title_full Cholesterolomics: An update
title_fullStr Cholesterolomics: An update
title_full_unstemmed Cholesterolomics: An update
title_short Cholesterolomics: An update
title_sort cholesterolomics: an update
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378159/
https://www.ncbi.nlm.nih.gov/pubmed/28087213
http://dx.doi.org/10.1016/j.ab.2017.01.009
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