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Biocompatible astaxanthin as a novel marine-oriented agent for dual chemo-photothermal therapy

The photothermal effect of a marine-oriented xanthophyll carotenoid, astaxanthin (AXT), was characterized based on its potential absorption of visible laser light and conversion of optical light energy into heat for thermal treatment. As an antioxidant and anticancer agent, AXT extracted from marine...

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Autores principales: Nguyen, Van Phuc, Kim, Sung Won, Kim, Hanna, Kim, Hyejin, Seok, Kwang Hyuk, Jung, Min Jung, Ahn, Yeh-chan, Kang, Hyun Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378353/
https://www.ncbi.nlm.nih.gov/pubmed/28369126
http://dx.doi.org/10.1371/journal.pone.0174687
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author Nguyen, Van Phuc
Kim, Sung Won
Kim, Hanna
Kim, Hyejin
Seok, Kwang Hyuk
Jung, Min Jung
Ahn, Yeh-chan
Kang, Hyun Wook
author_facet Nguyen, Van Phuc
Kim, Sung Won
Kim, Hanna
Kim, Hyejin
Seok, Kwang Hyuk
Jung, Min Jung
Ahn, Yeh-chan
Kang, Hyun Wook
author_sort Nguyen, Van Phuc
collection PubMed
description The photothermal effect of a marine-oriented xanthophyll carotenoid, astaxanthin (AXT), was characterized based on its potential absorption of visible laser light and conversion of optical light energy into heat for thermal treatment. As an antioxidant and anticancer agent, AXT extracted from marine material can be utilized for photothermal therapy due to its strong light absorption. The current study investigated the feasibility of the marine-based material AXT to increase the therapeutic efficacy of chemo-photothermal therapy (PTT) by assessing photothermal sessions in both cells and tumor tissues. A quasi-cw Q-switched 80 W 532 nm laser system was utilized to induce thermal necrosis in in vitro and in vivo models. An in vitro cytotoxicity study of AXT was implemented using squamous cell carcinoma (VX2) and macrophage (246.7) cell lines. In vivo PTT experiments were performed on 17 rabbits bearing VX2 tumors on their eyes that were treated with or without intratumoral injection of AXT at a dose of 100 μl (300 μg/ml) followed by laser irradiation at a low irradiance of 0.11 W/cm(2). Fluorescence microscopy images revealed cellular death via apoptosis and necrosis owing to the dual chemo-photothermal effects induced by AXT. In vivo experimental results demonstrated that the AXT-assisted irradiation entailed a temperature increase by 30.4°C after tumor treatment for 4 min. The relative variations in tumor volume confirmed that the tumors treated with both AXT and laser irradiation completely disappeared 14 days after treatment, but the tumors treated under other conditions gradually grew. Due to selective light absorption, AXT-assisted laser treatment could be an effective thermal therapy for various drug-resistant cancers.
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spelling pubmed-53783532017-04-07 Biocompatible astaxanthin as a novel marine-oriented agent for dual chemo-photothermal therapy Nguyen, Van Phuc Kim, Sung Won Kim, Hanna Kim, Hyejin Seok, Kwang Hyuk Jung, Min Jung Ahn, Yeh-chan Kang, Hyun Wook PLoS One Research Article The photothermal effect of a marine-oriented xanthophyll carotenoid, astaxanthin (AXT), was characterized based on its potential absorption of visible laser light and conversion of optical light energy into heat for thermal treatment. As an antioxidant and anticancer agent, AXT extracted from marine material can be utilized for photothermal therapy due to its strong light absorption. The current study investigated the feasibility of the marine-based material AXT to increase the therapeutic efficacy of chemo-photothermal therapy (PTT) by assessing photothermal sessions in both cells and tumor tissues. A quasi-cw Q-switched 80 W 532 nm laser system was utilized to induce thermal necrosis in in vitro and in vivo models. An in vitro cytotoxicity study of AXT was implemented using squamous cell carcinoma (VX2) and macrophage (246.7) cell lines. In vivo PTT experiments were performed on 17 rabbits bearing VX2 tumors on their eyes that were treated with or without intratumoral injection of AXT at a dose of 100 μl (300 μg/ml) followed by laser irradiation at a low irradiance of 0.11 W/cm(2). Fluorescence microscopy images revealed cellular death via apoptosis and necrosis owing to the dual chemo-photothermal effects induced by AXT. In vivo experimental results demonstrated that the AXT-assisted irradiation entailed a temperature increase by 30.4°C after tumor treatment for 4 min. The relative variations in tumor volume confirmed that the tumors treated with both AXT and laser irradiation completely disappeared 14 days after treatment, but the tumors treated under other conditions gradually grew. Due to selective light absorption, AXT-assisted laser treatment could be an effective thermal therapy for various drug-resistant cancers. Public Library of Science 2017-04-03 /pmc/articles/PMC5378353/ /pubmed/28369126 http://dx.doi.org/10.1371/journal.pone.0174687 Text en © 2017 Nguyen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nguyen, Van Phuc
Kim, Sung Won
Kim, Hanna
Kim, Hyejin
Seok, Kwang Hyuk
Jung, Min Jung
Ahn, Yeh-chan
Kang, Hyun Wook
Biocompatible astaxanthin as a novel marine-oriented agent for dual chemo-photothermal therapy
title Biocompatible astaxanthin as a novel marine-oriented agent for dual chemo-photothermal therapy
title_full Biocompatible astaxanthin as a novel marine-oriented agent for dual chemo-photothermal therapy
title_fullStr Biocompatible astaxanthin as a novel marine-oriented agent for dual chemo-photothermal therapy
title_full_unstemmed Biocompatible astaxanthin as a novel marine-oriented agent for dual chemo-photothermal therapy
title_short Biocompatible astaxanthin as a novel marine-oriented agent for dual chemo-photothermal therapy
title_sort biocompatible astaxanthin as a novel marine-oriented agent for dual chemo-photothermal therapy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378353/
https://www.ncbi.nlm.nih.gov/pubmed/28369126
http://dx.doi.org/10.1371/journal.pone.0174687
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