ANKRD54 preferentially selects Bruton’s Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library

Bruton’s Tyrosine Kinase (BTK) is a cytoplasmic protein tyrosine kinase with a fundamental role in B-lymphocyte development and activation. The nucleocytoplasmic shuttling of BTK is specifically modulated by the Ankyrin Repeat Domain 54 (ANKRD54) protein and the interaction is known to be exclusivel...

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Autores principales: Gustafsson, Manuela O., Mohammad, Dara K., Ylösmäki, Erkko, Choi, Hyunseok, Shrestha, Subhash, Wang, Qing, Nore, Beston F., Saksela, Kalle, Smith, C. I. Edvard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378395/
https://www.ncbi.nlm.nih.gov/pubmed/28369144
http://dx.doi.org/10.1371/journal.pone.0174909
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author Gustafsson, Manuela O.
Mohammad, Dara K.
Ylösmäki, Erkko
Choi, Hyunseok
Shrestha, Subhash
Wang, Qing
Nore, Beston F.
Saksela, Kalle
Smith, C. I. Edvard
author_facet Gustafsson, Manuela O.
Mohammad, Dara K.
Ylösmäki, Erkko
Choi, Hyunseok
Shrestha, Subhash
Wang, Qing
Nore, Beston F.
Saksela, Kalle
Smith, C. I. Edvard
author_sort Gustafsson, Manuela O.
collection PubMed
description Bruton’s Tyrosine Kinase (BTK) is a cytoplasmic protein tyrosine kinase with a fundamental role in B-lymphocyte development and activation. The nucleocytoplasmic shuttling of BTK is specifically modulated by the Ankyrin Repeat Domain 54 (ANKRD54) protein and the interaction is known to be exclusively SH3-dependent. To identify the spectrum of the ANKRD54 SH3-interactome, we applied phage-display screening of a library containing all the 296 human SH3 domains. The BTK-SH3 domain was the prime interactor. Quantitative western blotting analysis demonstrated the accuracy of the screening procedure. Revealing the spectrum and specificity of ANKRD54-interactome is a critical step toward functional analysis in cells and tissues.
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spelling pubmed-53783952017-04-07 ANKRD54 preferentially selects Bruton’s Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library Gustafsson, Manuela O. Mohammad, Dara K. Ylösmäki, Erkko Choi, Hyunseok Shrestha, Subhash Wang, Qing Nore, Beston F. Saksela, Kalle Smith, C. I. Edvard PLoS One Research Article Bruton’s Tyrosine Kinase (BTK) is a cytoplasmic protein tyrosine kinase with a fundamental role in B-lymphocyte development and activation. The nucleocytoplasmic shuttling of BTK is specifically modulated by the Ankyrin Repeat Domain 54 (ANKRD54) protein and the interaction is known to be exclusively SH3-dependent. To identify the spectrum of the ANKRD54 SH3-interactome, we applied phage-display screening of a library containing all the 296 human SH3 domains. The BTK-SH3 domain was the prime interactor. Quantitative western blotting analysis demonstrated the accuracy of the screening procedure. Revealing the spectrum and specificity of ANKRD54-interactome is a critical step toward functional analysis in cells and tissues. Public Library of Science 2017-04-03 /pmc/articles/PMC5378395/ /pubmed/28369144 http://dx.doi.org/10.1371/journal.pone.0174909 Text en © 2017 Gustafsson et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gustafsson, Manuela O.
Mohammad, Dara K.
Ylösmäki, Erkko
Choi, Hyunseok
Shrestha, Subhash
Wang, Qing
Nore, Beston F.
Saksela, Kalle
Smith, C. I. Edvard
ANKRD54 preferentially selects Bruton’s Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library
title ANKRD54 preferentially selects Bruton’s Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library
title_full ANKRD54 preferentially selects Bruton’s Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library
title_fullStr ANKRD54 preferentially selects Bruton’s Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library
title_full_unstemmed ANKRD54 preferentially selects Bruton’s Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library
title_short ANKRD54 preferentially selects Bruton’s Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library
title_sort ankrd54 preferentially selects bruton’s tyrosine kinase (btk) from a human src-homology 3 (sh3) domain library
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378395/
https://www.ncbi.nlm.nih.gov/pubmed/28369144
http://dx.doi.org/10.1371/journal.pone.0174909
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