Cargando…
Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action
Psoriasis is a chronic autoimmune disease that cannot be cured. It can however be controlled by various forms of treatment, including topical, systemic agents, and phototherapy. Topical treatment is the first-line treatment and favored by most physicians, as this form of therapy has more patient com...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378462/ https://www.ncbi.nlm.nih.gov/pubmed/28405165 http://dx.doi.org/10.2147/IJN.S125302 |
_version_ | 1782519448106696704 |
---|---|
author | Musa, Siti Hajar Basri, Mahiran Fard Masoumi, Hamid Reza Shamsudin, Norashikin Salim, Norazlinaliza |
author_facet | Musa, Siti Hajar Basri, Mahiran Fard Masoumi, Hamid Reza Shamsudin, Norashikin Salim, Norazlinaliza |
author_sort | Musa, Siti Hajar |
collection | PubMed |
description | Psoriasis is a chronic autoimmune disease that cannot be cured. It can however be controlled by various forms of treatment, including topical, systemic agents, and phototherapy. Topical treatment is the first-line treatment and favored by most physicians, as this form of therapy has more patient compliance. Introducing a nanoemulsion for transporting cyclosporine as an anti-inflammatory drug to an itchy site of skin disease would enhance the effectiveness of topical treatment for psoriasis. The addition of nutmeg and virgin coconut-oil mixture, with their unique properties, could improve cyclosporine loading and solubility. A high-shear homogenizer was used in formulating a cyclosporine-loaded nanoemulsion. A D-optimal mixture experimental design was used in the optimization of nanoemulsion compositions, in order to understand the relationships behind the effect of independent variables (oil, surfactant, xanthan gum, and water content) on physicochemical response (particle size and polydispersity index) and rheological response (viscosity and k-value). Investigation of these variables suggests two optimized formulations with specific oil (15% and 20%), surfactant (15%), xanthan gum (0.75%), and water content (67.55% and 62.55%), which possessed intended responses and good stability against separation over 3 months’ storage at different temperatures. Optimized nanoemulsions of pH 4.5 were further studied with all types of stability analysis: physical stability, coalescence-rate analysis, Ostwald ripening, and freeze–thaw cycles. In vitro release proved the efficacy of nanosize emulsions in carrying cyclosporine across rat skin and a synthetic membrane that best fit the Korsmeyer–Peppas kinetic model. In vivo skin analysis towards healthy volunteers showed a significant improvement in the stratum corneum in skin hydration. |
format | Online Article Text |
id | pubmed-5378462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53784622017-04-12 Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action Musa, Siti Hajar Basri, Mahiran Fard Masoumi, Hamid Reza Shamsudin, Norashikin Salim, Norazlinaliza Int J Nanomedicine Original Research Psoriasis is a chronic autoimmune disease that cannot be cured. It can however be controlled by various forms of treatment, including topical, systemic agents, and phototherapy. Topical treatment is the first-line treatment and favored by most physicians, as this form of therapy has more patient compliance. Introducing a nanoemulsion for transporting cyclosporine as an anti-inflammatory drug to an itchy site of skin disease would enhance the effectiveness of topical treatment for psoriasis. The addition of nutmeg and virgin coconut-oil mixture, with their unique properties, could improve cyclosporine loading and solubility. A high-shear homogenizer was used in formulating a cyclosporine-loaded nanoemulsion. A D-optimal mixture experimental design was used in the optimization of nanoemulsion compositions, in order to understand the relationships behind the effect of independent variables (oil, surfactant, xanthan gum, and water content) on physicochemical response (particle size and polydispersity index) and rheological response (viscosity and k-value). Investigation of these variables suggests two optimized formulations with specific oil (15% and 20%), surfactant (15%), xanthan gum (0.75%), and water content (67.55% and 62.55%), which possessed intended responses and good stability against separation over 3 months’ storage at different temperatures. Optimized nanoemulsions of pH 4.5 were further studied with all types of stability analysis: physical stability, coalescence-rate analysis, Ostwald ripening, and freeze–thaw cycles. In vitro release proved the efficacy of nanosize emulsions in carrying cyclosporine across rat skin and a synthetic membrane that best fit the Korsmeyer–Peppas kinetic model. In vivo skin analysis towards healthy volunteers showed a significant improvement in the stratum corneum in skin hydration. Dove Medical Press 2017-03-28 /pmc/articles/PMC5378462/ /pubmed/28405165 http://dx.doi.org/10.2147/IJN.S125302 Text en © 2017 Musa et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Musa, Siti Hajar Basri, Mahiran Fard Masoumi, Hamid Reza Shamsudin, Norashikin Salim, Norazlinaliza Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action |
title | Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action |
title_full | Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action |
title_fullStr | Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action |
title_full_unstemmed | Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action |
title_short | Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action |
title_sort | enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378462/ https://www.ncbi.nlm.nih.gov/pubmed/28405165 http://dx.doi.org/10.2147/IJN.S125302 |
work_keys_str_mv | AT musasitihajar enhancementofphysicochemicalpropertiesofnanocolloidalcarrierloadedwithcyclosporinefortopicaltreatmentofpsoriasisinvitrodiffusionandinvivohydratingaction AT basrimahiran enhancementofphysicochemicalpropertiesofnanocolloidalcarrierloadedwithcyclosporinefortopicaltreatmentofpsoriasisinvitrodiffusionandinvivohydratingaction AT fardmasoumihamidreza enhancementofphysicochemicalpropertiesofnanocolloidalcarrierloadedwithcyclosporinefortopicaltreatmentofpsoriasisinvitrodiffusionandinvivohydratingaction AT shamsudinnorashikin enhancementofphysicochemicalpropertiesofnanocolloidalcarrierloadedwithcyclosporinefortopicaltreatmentofpsoriasisinvitrodiffusionandinvivohydratingaction AT salimnorazlinaliza enhancementofphysicochemicalpropertiesofnanocolloidalcarrierloadedwithcyclosporinefortopicaltreatmentofpsoriasisinvitrodiffusionandinvivohydratingaction |