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Analysis of Ki67, HMGA1, MDM2, and RB expression in nonfunctioning pituitary adenomas
Nonfunctioning pituitary adenomas (NFPAs) are the most prevalent type of pituitary macro-adenoma. Clarifying the relationship between NFPA markers and disease progression or recurrence could provide a basis for administration of adjuvant treatments. The present study examined the expression levels o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer US
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378727/ https://www.ncbi.nlm.nih.gov/pubmed/28255749 http://dx.doi.org/10.1007/s11060-016-2365-9 |
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author | Yao, Xiaohui Gao, Hua Li, Chuzhong Wu, Lijuan Bai, Jiwei Wang, Jichao Li, Yangfang Zhang, Yazhuo |
author_facet | Yao, Xiaohui Gao, Hua Li, Chuzhong Wu, Lijuan Bai, Jiwei Wang, Jichao Li, Yangfang Zhang, Yazhuo |
author_sort | Yao, Xiaohui |
collection | PubMed |
description | Nonfunctioning pituitary adenomas (NFPAs) are the most prevalent type of pituitary macro-adenoma. Clarifying the relationship between NFPA markers and disease progression or recurrence could provide a basis for administration of adjuvant treatments. The present study examined the expression levels of high-mobility group (HMG)A1, Ki-67, mouse double minute 2 homolog (MDM2), and retinoblastoma (RB)with respect to NFPA recurrence. Immunohistochemistry was carried out using antibodies to Ki-67, MDM2, HMGA-1, and RB on tissue microarray slides of a cohort of 35 paired NFPA samples of primary and recurrence/regrowth tumors. Based on postoperative magnetic resonance imaging data, tumors were classified as recurrence (n = 20) included primary and recurrent tumors or regrowth (n = 15) included primary and regrowth tumors, which are paired. Protein expression was classified as negative or positive according to the H-score method and was analyzed with respect to clinical and pathological findings. MDM2-positive cases accounted for11/20 primary and 19/20 s recurrent tumors (χ(2) = 8.533, P = 0.003), and 9/15 primary tumors and 15/15 s regrowth tumors (χ(2) = 7.5, P = 0.006). MGA1-positive cases represented 9/20 primary tumors and 16/20 s recurrent tumors (χ(2) = 5.227, P = 0.022), and 4/15 primary tumors and 12/15 s regrowth tumors (χ(2) = 8.571, P = 0.003). There was no statistically significant difference in Ki-67 expression between primary and second recurrent/regrowth tumors although theKi67 labeling index was higher in the latter groups. RB was highly expressed in all groups with no significant difference between them. HMGA1 and MDM2 were more highly expressed in recurrence/regrowth cases of NFPA than in primary NFPA. HMGA1 and MDM2 are biomarkers and potential drug targets for NFPA treatment. |
format | Online Article Text |
id | pubmed-5378727 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-53787272017-04-17 Analysis of Ki67, HMGA1, MDM2, and RB expression in nonfunctioning pituitary adenomas Yao, Xiaohui Gao, Hua Li, Chuzhong Wu, Lijuan Bai, Jiwei Wang, Jichao Li, Yangfang Zhang, Yazhuo J Neurooncol Laboratory Investigation Nonfunctioning pituitary adenomas (NFPAs) are the most prevalent type of pituitary macro-adenoma. Clarifying the relationship between NFPA markers and disease progression or recurrence could provide a basis for administration of adjuvant treatments. The present study examined the expression levels of high-mobility group (HMG)A1, Ki-67, mouse double minute 2 homolog (MDM2), and retinoblastoma (RB)with respect to NFPA recurrence. Immunohistochemistry was carried out using antibodies to Ki-67, MDM2, HMGA-1, and RB on tissue microarray slides of a cohort of 35 paired NFPA samples of primary and recurrence/regrowth tumors. Based on postoperative magnetic resonance imaging data, tumors were classified as recurrence (n = 20) included primary and recurrent tumors or regrowth (n = 15) included primary and regrowth tumors, which are paired. Protein expression was classified as negative or positive according to the H-score method and was analyzed with respect to clinical and pathological findings. MDM2-positive cases accounted for11/20 primary and 19/20 s recurrent tumors (χ(2) = 8.533, P = 0.003), and 9/15 primary tumors and 15/15 s regrowth tumors (χ(2) = 7.5, P = 0.006). MGA1-positive cases represented 9/20 primary tumors and 16/20 s recurrent tumors (χ(2) = 5.227, P = 0.022), and 4/15 primary tumors and 12/15 s regrowth tumors (χ(2) = 8.571, P = 0.003). There was no statistically significant difference in Ki-67 expression between primary and second recurrent/regrowth tumors although theKi67 labeling index was higher in the latter groups. RB was highly expressed in all groups with no significant difference between them. HMGA1 and MDM2 were more highly expressed in recurrence/regrowth cases of NFPA than in primary NFPA. HMGA1 and MDM2 are biomarkers and potential drug targets for NFPA treatment. Springer US 2017-03-02 2017 /pmc/articles/PMC5378727/ /pubmed/28255749 http://dx.doi.org/10.1007/s11060-016-2365-9 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Laboratory Investigation Yao, Xiaohui Gao, Hua Li, Chuzhong Wu, Lijuan Bai, Jiwei Wang, Jichao Li, Yangfang Zhang, Yazhuo Analysis of Ki67, HMGA1, MDM2, and RB expression in nonfunctioning pituitary adenomas |
title | Analysis of Ki67, HMGA1, MDM2, and RB expression in nonfunctioning pituitary adenomas |
title_full | Analysis of Ki67, HMGA1, MDM2, and RB expression in nonfunctioning pituitary adenomas |
title_fullStr | Analysis of Ki67, HMGA1, MDM2, and RB expression in nonfunctioning pituitary adenomas |
title_full_unstemmed | Analysis of Ki67, HMGA1, MDM2, and RB expression in nonfunctioning pituitary adenomas |
title_short | Analysis of Ki67, HMGA1, MDM2, and RB expression in nonfunctioning pituitary adenomas |
title_sort | analysis of ki67, hmga1, mdm2, and rb expression in nonfunctioning pituitary adenomas |
topic | Laboratory Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378727/ https://www.ncbi.nlm.nih.gov/pubmed/28255749 http://dx.doi.org/10.1007/s11060-016-2365-9 |
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