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Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract
OBJECTIVE(S): Nowadays much effort is being invested in order to diagnose the mechanisms involved in neural differentiation. By clarifying this, making desired neural cells in vitro and applying them into diverse neurological disorders suffered from neural cell malfunctions could be a feasible choic...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378968/ https://www.ncbi.nlm.nih.gov/pubmed/28392903 http://dx.doi.org/10.22038/ijbms.2017.8360 |
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author | Jahromi, Iman Razeghian Mehrabani, Davood Mohammadi, Ali Seno, Mohammad Mahdi Ghahramani Dianatpour, Mehdi Zare, Shahrokh Tamadon, Amin |
author_facet | Jahromi, Iman Razeghian Mehrabani, Davood Mohammadi, Ali Seno, Mohammad Mahdi Ghahramani Dianatpour, Mehdi Zare, Shahrokh Tamadon, Amin |
author_sort | Jahromi, Iman Razeghian |
collection | PubMed |
description | OBJECTIVE(S): Nowadays much effort is being invested in order to diagnose the mechanisms involved in neural differentiation. By clarifying this, making desired neural cells in vitro and applying them into diverse neurological disorders suffered from neural cell malfunctions could be a feasible choice. Thus, the present study assessed the capability of fetal brain extract (FBE) to induce rat bone marrow-derived mesenchymal stem cells (BM-MSCs) toward neural cells. MATERIALS AND METHODS: For this purpose, BM-MSCs were collected from rats and cultured and their mesenchymal properties were confirmed. After exposure of the BM-MSCs to fetal brain extract, the cells were evaluated and harvested at days 3 and 7 after treatment. RESULTS: The BM-MSCs that were exposed to FBE changed their appearance dramatically from spindle shape to cells with dendrite-like processes. Those neural like processes were absent in the control group. In addition, a neural specific marker, vimentin, was expressed significantly in the treatment group but not in the negative control group. CONCLUSION: This study presented the FBE as a natural neural differentiation agent, which probably has required factors for making neurons. In addition, vimentin overexpression was observed in the treated group which confirms neuron-like cell differentiation of BM-MSCs after induction. |
format | Online Article Text |
id | pubmed-5378968 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-53789682017-04-07 Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract Jahromi, Iman Razeghian Mehrabani, Davood Mohammadi, Ali Seno, Mohammad Mahdi Ghahramani Dianatpour, Mehdi Zare, Shahrokh Tamadon, Amin Iran J Basic Med Sci Original Article OBJECTIVE(S): Nowadays much effort is being invested in order to diagnose the mechanisms involved in neural differentiation. By clarifying this, making desired neural cells in vitro and applying them into diverse neurological disorders suffered from neural cell malfunctions could be a feasible choice. Thus, the present study assessed the capability of fetal brain extract (FBE) to induce rat bone marrow-derived mesenchymal stem cells (BM-MSCs) toward neural cells. MATERIALS AND METHODS: For this purpose, BM-MSCs were collected from rats and cultured and their mesenchymal properties were confirmed. After exposure of the BM-MSCs to fetal brain extract, the cells were evaluated and harvested at days 3 and 7 after treatment. RESULTS: The BM-MSCs that were exposed to FBE changed their appearance dramatically from spindle shape to cells with dendrite-like processes. Those neural like processes were absent in the control group. In addition, a neural specific marker, vimentin, was expressed significantly in the treatment group but not in the negative control group. CONCLUSION: This study presented the FBE as a natural neural differentiation agent, which probably has required factors for making neurons. In addition, vimentin overexpression was observed in the treated group which confirms neuron-like cell differentiation of BM-MSCs after induction. Mashhad University of Medical Sciences 2017-03 /pmc/articles/PMC5378968/ /pubmed/28392903 http://dx.doi.org/10.22038/ijbms.2017.8360 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Jahromi, Iman Razeghian Mehrabani, Davood Mohammadi, Ali Seno, Mohammad Mahdi Ghahramani Dianatpour, Mehdi Zare, Shahrokh Tamadon, Amin Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract |
title | Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract |
title_full | Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract |
title_fullStr | Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract |
title_full_unstemmed | Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract |
title_short | Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract |
title_sort | emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378968/ https://www.ncbi.nlm.nih.gov/pubmed/28392903 http://dx.doi.org/10.22038/ijbms.2017.8360 |
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