XTEND: Extending the depth of field in cryo soft X-ray tomography

We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard re...

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Detalles Bibliográficos
Autores principales: Otón, Joaquín, Pereiro, Eva, Conesa, José J., Chichón, Francisco J., Luque, Daniel, Rodríguez, Javier M., Pérez-Berná, Ana J., Sorzano, Carlos Oscar S., Klukowska, Joanna, Herman, Gabor T., Vargas, Javier, Marabini, Roberto, Carrascosa, José L., Carazo, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379191/
https://www.ncbi.nlm.nih.gov/pubmed/28374769
http://dx.doi.org/10.1038/srep45808
Descripción
Sumario:We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.

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