XTEND: Extending the depth of field in cryo soft X-ray tomography

We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard re...

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Autores principales: Otón, Joaquín, Pereiro, Eva, Conesa, José J., Chichón, Francisco J., Luque, Daniel, Rodríguez, Javier M., Pérez-Berná, Ana J., Sorzano, Carlos Oscar S., Klukowska, Joanna, Herman, Gabor T., Vargas, Javier, Marabini, Roberto, Carrascosa, José L., Carazo, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379191/
https://www.ncbi.nlm.nih.gov/pubmed/28374769
http://dx.doi.org/10.1038/srep45808
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author Otón, Joaquín
Pereiro, Eva
Conesa, José J.
Chichón, Francisco J.
Luque, Daniel
Rodríguez, Javier M.
Pérez-Berná, Ana J.
Sorzano, Carlos Oscar S.
Klukowska, Joanna
Herman, Gabor T.
Vargas, Javier
Marabini, Roberto
Carrascosa, José L.
Carazo, José M.
author_facet Otón, Joaquín
Pereiro, Eva
Conesa, José J.
Chichón, Francisco J.
Luque, Daniel
Rodríguez, Javier M.
Pérez-Berná, Ana J.
Sorzano, Carlos Oscar S.
Klukowska, Joanna
Herman, Gabor T.
Vargas, Javier
Marabini, Roberto
Carrascosa, José L.
Carazo, José M.
author_sort Otón, Joaquín
collection PubMed
description We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.
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spelling pubmed-53791912017-04-10 XTEND: Extending the depth of field in cryo soft X-ray tomography Otón, Joaquín Pereiro, Eva Conesa, José J. Chichón, Francisco J. Luque, Daniel Rodríguez, Javier M. Pérez-Berná, Ana J. Sorzano, Carlos Oscar S. Klukowska, Joanna Herman, Gabor T. Vargas, Javier Marabini, Roberto Carrascosa, José L. Carazo, José M. Sci Rep Article We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters. Nature Publishing Group 2017-04-04 /pmc/articles/PMC5379191/ /pubmed/28374769 http://dx.doi.org/10.1038/srep45808 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Otón, Joaquín
Pereiro, Eva
Conesa, José J.
Chichón, Francisco J.
Luque, Daniel
Rodríguez, Javier M.
Pérez-Berná, Ana J.
Sorzano, Carlos Oscar S.
Klukowska, Joanna
Herman, Gabor T.
Vargas, Javier
Marabini, Roberto
Carrascosa, José L.
Carazo, José M.
XTEND: Extending the depth of field in cryo soft X-ray tomography
title XTEND: Extending the depth of field in cryo soft X-ray tomography
title_full XTEND: Extending the depth of field in cryo soft X-ray tomography
title_fullStr XTEND: Extending the depth of field in cryo soft X-ray tomography
title_full_unstemmed XTEND: Extending the depth of field in cryo soft X-ray tomography
title_short XTEND: Extending the depth of field in cryo soft X-ray tomography
title_sort xtend: extending the depth of field in cryo soft x-ray tomography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379191/
https://www.ncbi.nlm.nih.gov/pubmed/28374769
http://dx.doi.org/10.1038/srep45808
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