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Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice
BACKGROUND: Dengue is the most prevalent arthropod-borne viral disease in the world. In this article we present results on the development, characterization and immunogenic evaluation of an alternative vaccine candidate against Dengue. METHODS: The MWNT-DENV3E nanoconjugate was developed by covalent...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379608/ https://www.ncbi.nlm.nih.gov/pubmed/28376812 http://dx.doi.org/10.1186/s12951-017-0259-4 |
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author | Versiani, Alice F. Astigarraga, Ruiz G. Rocha, Eliseu S. O. Barboza, Ana Paula M. Kroon, Erna G. Rachid, Milene A. Souza, Daniele G. Ladeira, Luiz O. Barbosa-Stancioli, Edel F. Jorio, Ado Da Fonseca, Flávio G. |
author_facet | Versiani, Alice F. Astigarraga, Ruiz G. Rocha, Eliseu S. O. Barboza, Ana Paula M. Kroon, Erna G. Rachid, Milene A. Souza, Daniele G. Ladeira, Luiz O. Barbosa-Stancioli, Edel F. Jorio, Ado Da Fonseca, Flávio G. |
author_sort | Versiani, Alice F. |
collection | PubMed |
description | BACKGROUND: Dengue is the most prevalent arthropod-borne viral disease in the world. In this article we present results on the development, characterization and immunogenic evaluation of an alternative vaccine candidate against Dengue. METHODS: The MWNT-DENV3E nanoconjugate was developed by covalent functionalization of carboxylated multi-walled carbon nanotubes (MWNT) with recombinant dengue envelope (DENV3E) proteins. The recombinant antigens were bound to the MWNT using a diimide-activated amidation process and the immunogen was characterized by TEM, AFM and Raman Spectroscopy. Furthermore, the immunogenicity of this vaccine candidate was evaluated in a murine model. RESULTS: Immunization with MWNT-DENV3E induced comparable IgG responses in relation to the immunization with non-conjugated proteins; however, the inoculation of the nanoconjugate into mice generated higher titers of neutralizing antibodies. Cell-mediated responses were also evaluated, and higher dengue-specific splenocyte proliferation was observed in cell cultures derived from mice immunized with MWNT-DENV3E when compared to animals immunized with the non-conjugated DENV3E. CONCLUSIONS: Despite the recent licensure of the CYD-TDV dengue vaccine in some countries, results from the vaccine’s phase III trial have cast doubts about its overall efficacy and global applicability. While questions about the effectiveness of the CYD-TDV vaccine still lingers, it is wise to keep at hand an array of vaccine candidates, including alternative non-classical approaches like the one presented here. |
format | Online Article Text |
id | pubmed-5379608 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-53796082017-04-07 Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice Versiani, Alice F. Astigarraga, Ruiz G. Rocha, Eliseu S. O. Barboza, Ana Paula M. Kroon, Erna G. Rachid, Milene A. Souza, Daniele G. Ladeira, Luiz O. Barbosa-Stancioli, Edel F. Jorio, Ado Da Fonseca, Flávio G. J Nanobiotechnology Research BACKGROUND: Dengue is the most prevalent arthropod-borne viral disease in the world. In this article we present results on the development, characterization and immunogenic evaluation of an alternative vaccine candidate against Dengue. METHODS: The MWNT-DENV3E nanoconjugate was developed by covalent functionalization of carboxylated multi-walled carbon nanotubes (MWNT) with recombinant dengue envelope (DENV3E) proteins. The recombinant antigens were bound to the MWNT using a diimide-activated amidation process and the immunogen was characterized by TEM, AFM and Raman Spectroscopy. Furthermore, the immunogenicity of this vaccine candidate was evaluated in a murine model. RESULTS: Immunization with MWNT-DENV3E induced comparable IgG responses in relation to the immunization with non-conjugated proteins; however, the inoculation of the nanoconjugate into mice generated higher titers of neutralizing antibodies. Cell-mediated responses were also evaluated, and higher dengue-specific splenocyte proliferation was observed in cell cultures derived from mice immunized with MWNT-DENV3E when compared to animals immunized with the non-conjugated DENV3E. CONCLUSIONS: Despite the recent licensure of the CYD-TDV dengue vaccine in some countries, results from the vaccine’s phase III trial have cast doubts about its overall efficacy and global applicability. While questions about the effectiveness of the CYD-TDV vaccine still lingers, it is wise to keep at hand an array of vaccine candidates, including alternative non-classical approaches like the one presented here. BioMed Central 2017-04-04 /pmc/articles/PMC5379608/ /pubmed/28376812 http://dx.doi.org/10.1186/s12951-017-0259-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Versiani, Alice F. Astigarraga, Ruiz G. Rocha, Eliseu S. O. Barboza, Ana Paula M. Kroon, Erna G. Rachid, Milene A. Souza, Daniele G. Ladeira, Luiz O. Barbosa-Stancioli, Edel F. Jorio, Ado Da Fonseca, Flávio G. Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
title | Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
title_full | Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
title_fullStr | Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
title_full_unstemmed | Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
title_short | Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
title_sort | multi-walled carbon nanotubes functionalized with recombinant dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379608/ https://www.ncbi.nlm.nih.gov/pubmed/28376812 http://dx.doi.org/10.1186/s12951-017-0259-4 |
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