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The newly identified migration inhibitory protein regulates the radial migration in the developing neocortex

Neuronal migration is a crucial process in the organization of the developing cerebral cortex. Although a number of positive regulatory mechanisms of radial migration have been identified, negative cell-autonomous mechanisms have yet to be fully described. Here we report a newly identified Migration...

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Autores principales: Zhang, Suxiang, Kanemitsu, Yoshitaka, Fujitani, Masashi, Yamashita, Toshihide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380009/
https://www.ncbi.nlm.nih.gov/pubmed/25099998
http://dx.doi.org/10.1038/srep05984
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author Zhang, Suxiang
Kanemitsu, Yoshitaka
Fujitani, Masashi
Yamashita, Toshihide
author_facet Zhang, Suxiang
Kanemitsu, Yoshitaka
Fujitani, Masashi
Yamashita, Toshihide
author_sort Zhang, Suxiang
collection PubMed
description Neuronal migration is a crucial process in the organization of the developing cerebral cortex. Although a number of positive regulatory mechanisms of radial migration have been identified, negative cell-autonomous mechanisms have yet to be fully described. Here we report a newly identified Migration Inhibitory Protein (MINP, formerly known as 2900011O08Rik) that negatively regulates radial migration. MINP mRNA was specifically detected in the central and peripheral nervous system, and especially enriched in the cerebral cortex. MINP immunoreactivity co-localized with the neuronal marker Tuj1 and was detected in the cytoplasm of post-mitotic neurons. To elucidate the function of MINP in the developing brain, we performed in utero electroporation of MINP siRNA, MINP shRNA, or MINP-overexpressing vectors into mouse cortices and carried out in vivo migration assays. Whereas knockdown of MINP did not alter neuronal morphology, the radial migration was found accelerated by MINP knockdown, and reduced by MINP overexpression. This migration phenotype was also confirmed in vitro, indicating that MINP regulates neuronal migration in a cell-autonomous fashion. Furthermore, downregulation of MINP affected microtubule stability by interacting with tubulin that is a potential mechanism involved in the regulation of neuronal migration.
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spelling pubmed-53800092017-04-10 The newly identified migration inhibitory protein regulates the radial migration in the developing neocortex Zhang, Suxiang Kanemitsu, Yoshitaka Fujitani, Masashi Yamashita, Toshihide Sci Rep Article Neuronal migration is a crucial process in the organization of the developing cerebral cortex. Although a number of positive regulatory mechanisms of radial migration have been identified, negative cell-autonomous mechanisms have yet to be fully described. Here we report a newly identified Migration Inhibitory Protein (MINP, formerly known as 2900011O08Rik) that negatively regulates radial migration. MINP mRNA was specifically detected in the central and peripheral nervous system, and especially enriched in the cerebral cortex. MINP immunoreactivity co-localized with the neuronal marker Tuj1 and was detected in the cytoplasm of post-mitotic neurons. To elucidate the function of MINP in the developing brain, we performed in utero electroporation of MINP siRNA, MINP shRNA, or MINP-overexpressing vectors into mouse cortices and carried out in vivo migration assays. Whereas knockdown of MINP did not alter neuronal morphology, the radial migration was found accelerated by MINP knockdown, and reduced by MINP overexpression. This migration phenotype was also confirmed in vitro, indicating that MINP regulates neuronal migration in a cell-autonomous fashion. Furthermore, downregulation of MINP affected microtubule stability by interacting with tubulin that is a potential mechanism involved in the regulation of neuronal migration. Nature Publishing Group 2014-08-07 /pmc/articles/PMC5380009/ /pubmed/25099998 http://dx.doi.org/10.1038/srep05984 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhang, Suxiang
Kanemitsu, Yoshitaka
Fujitani, Masashi
Yamashita, Toshihide
The newly identified migration inhibitory protein regulates the radial migration in the developing neocortex
title The newly identified migration inhibitory protein regulates the radial migration in the developing neocortex
title_full The newly identified migration inhibitory protein regulates the radial migration in the developing neocortex
title_fullStr The newly identified migration inhibitory protein regulates the radial migration in the developing neocortex
title_full_unstemmed The newly identified migration inhibitory protein regulates the radial migration in the developing neocortex
title_short The newly identified migration inhibitory protein regulates the radial migration in the developing neocortex
title_sort newly identified migration inhibitory protein regulates the radial migration in the developing neocortex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380009/
https://www.ncbi.nlm.nih.gov/pubmed/25099998
http://dx.doi.org/10.1038/srep05984
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