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MicroRNA profiling of CD3(+)CD56(+) cytokine-induced killer cells

Studies have proven that IL-2 and IL-15 showed contrasting roles during CIK cells preparation. By employing microarray, we analyzed miRNA expression profiles of PBMC, CIK(IL-2) and CIK(IL-15). Advanced bioinformatic analyses were performed to explore the key miRNAs which may regulate cell proliferat...

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Autores principales: Wang, Wenju, Li, Ruhong, Meng, Mingyao, Wei, Chuanyu, Xie, Yanhua, Zhang, Yayong, Jiang, Lihong, Dong, Ruiyi, Wang, Chunhui, Zhong, Yiming, Yang, Fang, Tang, Weiwei, Jin, Xingfang, Liu, Baohua, Hou, Zongliu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380330/
https://www.ncbi.nlm.nih.gov/pubmed/25826780
http://dx.doi.org/10.1038/srep09571
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author Wang, Wenju
Li, Ruhong
Meng, Mingyao
Wei, Chuanyu
Xie, Yanhua
Zhang, Yayong
Jiang, Lihong
Dong, Ruiyi
Wang, Chunhui
Zhong, Yiming
Yang, Fang
Tang, Weiwei
Jin, Xingfang
Liu, Baohua
Hou, Zongliu
author_facet Wang, Wenju
Li, Ruhong
Meng, Mingyao
Wei, Chuanyu
Xie, Yanhua
Zhang, Yayong
Jiang, Lihong
Dong, Ruiyi
Wang, Chunhui
Zhong, Yiming
Yang, Fang
Tang, Weiwei
Jin, Xingfang
Liu, Baohua
Hou, Zongliu
author_sort Wang, Wenju
collection PubMed
description Studies have proven that IL-2 and IL-15 showed contrasting roles during CIK cells preparation. By employing microarray, we analyzed miRNA expression profiles of PBMC, CIK(IL-2) and CIK(IL-15). Advanced bioinformatic analyses were performed to explore the key miRNAs which may regulate cell proliferation and anti-tumor activity of CIK. We identified 261 differentially expressed miRNAs (DEMs) between PBMC and CIK(IL-2), and 249 DEMs between PBMC and CIK(IL-15). MiR-143-3p/miR-145-5p was miRNA cluster which may positively regulate cell proliferation. In contrast, miR-340-5p/miR-340-3p cluster may negatively regulate cell proliferation via induction apoptosis, which may cause decreased cell proliferation capacity of CIK(IL-2.) MiRNA-target interaction analysis indicated that 10 co-downregulated miRNAs may synergistically turn on the expression of a pool of tumor cytotoxic genes in CIK cells. The DEMs between CIK(IL-2) and CIK(IL-15) may contribute to enhanced tumor cytotoxic capacity of CIK(IL-2). Importantly, we found that repressed miR-193a-5p may regulate the expressions of inhibitory receptor KLRD1. The results of the validation assay have shown that KLRD1 were upregulated in CIK cells. Our findings have provided new insights into mechanisms of CIK cells production and tumor cytotoxic function, and shed light on their safety for clinical trial.
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spelling pubmed-53803302017-04-11 MicroRNA profiling of CD3(+)CD56(+) cytokine-induced killer cells Wang, Wenju Li, Ruhong Meng, Mingyao Wei, Chuanyu Xie, Yanhua Zhang, Yayong Jiang, Lihong Dong, Ruiyi Wang, Chunhui Zhong, Yiming Yang, Fang Tang, Weiwei Jin, Xingfang Liu, Baohua Hou, Zongliu Sci Rep Article Studies have proven that IL-2 and IL-15 showed contrasting roles during CIK cells preparation. By employing microarray, we analyzed miRNA expression profiles of PBMC, CIK(IL-2) and CIK(IL-15). Advanced bioinformatic analyses were performed to explore the key miRNAs which may regulate cell proliferation and anti-tumor activity of CIK. We identified 261 differentially expressed miRNAs (DEMs) between PBMC and CIK(IL-2), and 249 DEMs between PBMC and CIK(IL-15). MiR-143-3p/miR-145-5p was miRNA cluster which may positively regulate cell proliferation. In contrast, miR-340-5p/miR-340-3p cluster may negatively regulate cell proliferation via induction apoptosis, which may cause decreased cell proliferation capacity of CIK(IL-2.) MiRNA-target interaction analysis indicated that 10 co-downregulated miRNAs may synergistically turn on the expression of a pool of tumor cytotoxic genes in CIK cells. The DEMs between CIK(IL-2) and CIK(IL-15) may contribute to enhanced tumor cytotoxic capacity of CIK(IL-2). Importantly, we found that repressed miR-193a-5p may regulate the expressions of inhibitory receptor KLRD1. The results of the validation assay have shown that KLRD1 were upregulated in CIK cells. Our findings have provided new insights into mechanisms of CIK cells production and tumor cytotoxic function, and shed light on their safety for clinical trial. Nature Publishing Group 2015-03-31 /pmc/articles/PMC5380330/ /pubmed/25826780 http://dx.doi.org/10.1038/srep09571 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Wenju
Li, Ruhong
Meng, Mingyao
Wei, Chuanyu
Xie, Yanhua
Zhang, Yayong
Jiang, Lihong
Dong, Ruiyi
Wang, Chunhui
Zhong, Yiming
Yang, Fang
Tang, Weiwei
Jin, Xingfang
Liu, Baohua
Hou, Zongliu
MicroRNA profiling of CD3(+)CD56(+) cytokine-induced killer cells
title MicroRNA profiling of CD3(+)CD56(+) cytokine-induced killer cells
title_full MicroRNA profiling of CD3(+)CD56(+) cytokine-induced killer cells
title_fullStr MicroRNA profiling of CD3(+)CD56(+) cytokine-induced killer cells
title_full_unstemmed MicroRNA profiling of CD3(+)CD56(+) cytokine-induced killer cells
title_short MicroRNA profiling of CD3(+)CD56(+) cytokine-induced killer cells
title_sort microrna profiling of cd3(+)cd56(+) cytokine-induced killer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380330/
https://www.ncbi.nlm.nih.gov/pubmed/25826780
http://dx.doi.org/10.1038/srep09571
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