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Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter
As Pichia pastoris (syn. Komagataella sp.) yeast can secrete pure recombinant proteins at high rates, it is a desirable production system. The function of a novel synthetic variant of the AOX1 promoter was characterised comprehensively using a strain secreting Candida antarctica lipase B (CALB) as a...
Autores principales: | Looser, Verena, Lüthy, Dominik, Straumann, Marcel, Hecht, Katrin, Melzoch, Karel, Kovar, Karin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380701/ https://www.ncbi.nlm.nih.gov/pubmed/28130631 http://dx.doi.org/10.1007/s00253-017-8123-x |
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