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Structural Changes of the Urinary Bladder After Chronic Complete Spinal Cord Injury in Minipigs
PURPOSE: The aim of this study was to determine the structural changes of the urinary bladder after chronic spinal cord injury (SCI) in minipigs with the primary focus on the analysis of urinary bladder wall proteins and their quantitative distribution. METHODS: Seven Göttingen minipigs (adult, fema...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Continence Society
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380823/ https://www.ncbi.nlm.nih.gov/pubmed/28361517 http://dx.doi.org/10.5213/inj.1732666.333 |
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author | Foditsch, Elena E. Roider, Karin Patras, Irina Hutu, Ioan Bauer, Sophina Janetschek, Günter Zimmermann, Reinhold |
author_facet | Foditsch, Elena E. Roider, Karin Patras, Irina Hutu, Ioan Bauer, Sophina Janetschek, Günter Zimmermann, Reinhold |
author_sort | Foditsch, Elena E. |
collection | PubMed |
description | PURPOSE: The aim of this study was to determine the structural changes of the urinary bladder after chronic spinal cord injury (SCI) in minipigs with the primary focus on the analysis of urinary bladder wall proteins and their quantitative distribution. METHODS: Seven Göttingen minipigs (adult, female) underwent a complete spinal cord transection. Follow-up time was 4 months during which the bladder was drained by frequent single catheterisation and data from the bladder diary and daily urine strip test were collected. Samples from the urinary bladder were taken, fixed in 4% paraformaldehyde and stained for histological analyses. Bladder wall thickness, single tissue quantities/distributions, types I and III collagen, and elastin quantifications were performed. Comparisons to healthy urinary bladder tissue of age-matched minipigs were performed for statistical analyses. RESULTS: No urinary tract infections were observed in our SCI minipig collective during follow-up. A trend towards a reduction in bladder volumes and an increase in incontinence periods were seen. The bladder wall thickness significantly increased after chronic SCI. Furthermore, bladder wall composition was severely altered by a significant loss of smooth muscle tissue and a significant increase in connective tissue. Elastic fibres were reduced in number and altered in their structural appearance after SCI. Type I collagen was significantly increased, while type III collagen was significantly decreased after SCI. CONCLUSIONS: Chronic SCI highlighted that the urinary bladder wall undergoes fibrotic events with reduced contractile and elastic properties due to changes of the bladder wall protein composition. These changes show in detail how SCI severely influences the urinary bladder wall composition and depicts the similarities between minipigs and humans. |
format | Online Article Text |
id | pubmed-5380823 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Korean Continence Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-53808232017-04-05 Structural Changes of the Urinary Bladder After Chronic Complete Spinal Cord Injury in Minipigs Foditsch, Elena E. Roider, Karin Patras, Irina Hutu, Ioan Bauer, Sophina Janetschek, Günter Zimmermann, Reinhold Int Neurourol J Original Article PURPOSE: The aim of this study was to determine the structural changes of the urinary bladder after chronic spinal cord injury (SCI) in minipigs with the primary focus on the analysis of urinary bladder wall proteins and their quantitative distribution. METHODS: Seven Göttingen minipigs (adult, female) underwent a complete spinal cord transection. Follow-up time was 4 months during which the bladder was drained by frequent single catheterisation and data from the bladder diary and daily urine strip test were collected. Samples from the urinary bladder were taken, fixed in 4% paraformaldehyde and stained for histological analyses. Bladder wall thickness, single tissue quantities/distributions, types I and III collagen, and elastin quantifications were performed. Comparisons to healthy urinary bladder tissue of age-matched minipigs were performed for statistical analyses. RESULTS: No urinary tract infections were observed in our SCI minipig collective during follow-up. A trend towards a reduction in bladder volumes and an increase in incontinence periods were seen. The bladder wall thickness significantly increased after chronic SCI. Furthermore, bladder wall composition was severely altered by a significant loss of smooth muscle tissue and a significant increase in connective tissue. Elastic fibres were reduced in number and altered in their structural appearance after SCI. Type I collagen was significantly increased, while type III collagen was significantly decreased after SCI. CONCLUSIONS: Chronic SCI highlighted that the urinary bladder wall undergoes fibrotic events with reduced contractile and elastic properties due to changes of the bladder wall protein composition. These changes show in detail how SCI severely influences the urinary bladder wall composition and depicts the similarities between minipigs and humans. Korean Continence Society 2017-03 2017-03-24 /pmc/articles/PMC5380823/ /pubmed/28361517 http://dx.doi.org/10.5213/inj.1732666.333 Text en Copyright © 2017 Korean Continence Society This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Foditsch, Elena E. Roider, Karin Patras, Irina Hutu, Ioan Bauer, Sophina Janetschek, Günter Zimmermann, Reinhold Structural Changes of the Urinary Bladder After Chronic Complete Spinal Cord Injury in Minipigs |
title | Structural Changes of the Urinary Bladder After Chronic Complete Spinal Cord Injury in Minipigs |
title_full | Structural Changes of the Urinary Bladder After Chronic Complete Spinal Cord Injury in Minipigs |
title_fullStr | Structural Changes of the Urinary Bladder After Chronic Complete Spinal Cord Injury in Minipigs |
title_full_unstemmed | Structural Changes of the Urinary Bladder After Chronic Complete Spinal Cord Injury in Minipigs |
title_short | Structural Changes of the Urinary Bladder After Chronic Complete Spinal Cord Injury in Minipigs |
title_sort | structural changes of the urinary bladder after chronic complete spinal cord injury in minipigs |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380823/ https://www.ncbi.nlm.nih.gov/pubmed/28361517 http://dx.doi.org/10.5213/inj.1732666.333 |
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