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An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1

The rapid and accurate quantification of the pathogenic bacteria is extremely critical to decrease the bacterial infections in all areas related to health and safety. We have developed an electrochemical strategy for simultaneous ultrasensitive detection of E. coli O157:H7 and Vibrio cholerae O1. Th...

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Detalles Bibliográficos
Autores principales: Li, Yan, Xiong, Ya, Fang, Lichao, Jiang, Lili, Huang, Hui, Deng, Jun, Liang, Wenbin, Zheng, Junsong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381255/
https://www.ncbi.nlm.nih.gov/pubmed/28382165
http://dx.doi.org/10.7150/thno.17544
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author Li, Yan
Xiong, Ya
Fang, Lichao
Jiang, Lili
Huang, Hui
Deng, Jun
Liang, Wenbin
Zheng, Junsong
author_facet Li, Yan
Xiong, Ya
Fang, Lichao
Jiang, Lili
Huang, Hui
Deng, Jun
Liang, Wenbin
Zheng, Junsong
author_sort Li, Yan
collection PubMed
description The rapid and accurate quantification of the pathogenic bacteria is extremely critical to decrease the bacterial infections in all areas related to health and safety. We have developed an electrochemical strategy for simultaneous ultrasensitive detection of E. coli O157:H7 and Vibrio cholerae O1. This approach was based on the specific immune recognition of different pathogenic bacteria by multifunctional nanoconjugates and subsequent signal amplification. By employing the proposed biosensor, the concentrations of these pathogenic bacteria could be established on a single interface in a single run with improved sensitivity and accuracy. The successful approach of the simultaneous detection and quantification of two bacteria by an electrochemical biosensor demonstrated here could be readily expanded for the estimation of a variety of other pathogenic bacteria, proteins, and nucleotides. Because of their high sensitivity, electrochemical biosensors may represent a new avenue for early diagnosis of diseases.
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spelling pubmed-53812552017-04-05 An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1 Li, Yan Xiong, Ya Fang, Lichao Jiang, Lili Huang, Hui Deng, Jun Liang, Wenbin Zheng, Junsong Theranostics Research Paper The rapid and accurate quantification of the pathogenic bacteria is extremely critical to decrease the bacterial infections in all areas related to health and safety. We have developed an electrochemical strategy for simultaneous ultrasensitive detection of E. coli O157:H7 and Vibrio cholerae O1. This approach was based on the specific immune recognition of different pathogenic bacteria by multifunctional nanoconjugates and subsequent signal amplification. By employing the proposed biosensor, the concentrations of these pathogenic bacteria could be established on a single interface in a single run with improved sensitivity and accuracy. The successful approach of the simultaneous detection and quantification of two bacteria by an electrochemical biosensor demonstrated here could be readily expanded for the estimation of a variety of other pathogenic bacteria, proteins, and nucleotides. Because of their high sensitivity, electrochemical biosensors may represent a new avenue for early diagnosis of diseases. Ivyspring International Publisher 2017-02-21 /pmc/articles/PMC5381255/ /pubmed/28382165 http://dx.doi.org/10.7150/thno.17544 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Li, Yan
Xiong, Ya
Fang, Lichao
Jiang, Lili
Huang, Hui
Deng, Jun
Liang, Wenbin
Zheng, Junsong
An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1
title An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1
title_full An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1
title_fullStr An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1
title_full_unstemmed An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1
title_short An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1
title_sort electrochemical strategy using multifunctional nanoconjugates for efficient simultaneous detection of escherichia coli o157: h7 and vibrio cholerae o1
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381255/
https://www.ncbi.nlm.nih.gov/pubmed/28382165
http://dx.doi.org/10.7150/thno.17544
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