Estimating the number and size of phloem sieve plate pores using longitudinal views and geometric reconstruction

Because it is difficult to obtain transverse views of the plant phloem sieve plate pores, which are short tubes, to estimate their number and diameters, a method based on longitudinal views is proposed. This method uses recent methods to estimate the number and the sizes of approximately circular ob...

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Autor principal: Bussières, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381363/
https://www.ncbi.nlm.nih.gov/pubmed/24816291
http://dx.doi.org/10.1038/srep04929
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author Bussières, Philippe
author_facet Bussières, Philippe
author_sort Bussières, Philippe
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description Because it is difficult to obtain transverse views of the plant phloem sieve plate pores, which are short tubes, to estimate their number and diameters, a method based on longitudinal views is proposed. This method uses recent methods to estimate the number and the sizes of approximately circular objects from their images, given by slices perpendicular to the objects. Moreover, because such longitudinal views are obtained from slices that are rather close to the plate centres whereas the pore size may vary with the pore distance from the plate edge, a sieve plate reconstruction model was developed and incorporated in the method to consider this bias. The method was successfully tested with published longitudinal views of phloem of Soybean and an exceptional entire transverse view from the same tissue. The method was also validated with simulated slices in two sieve plates from Cucurbita and Phaseolus. This method will likely be useful to estimate and to model the hydraulic conductivity and the architecture of the plant phloem, and it could have applications for other materials with approximately cylindrical structures.
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spelling pubmed-53813632017-04-11 Estimating the number and size of phloem sieve plate pores using longitudinal views and geometric reconstruction Bussières, Philippe Sci Rep Article Because it is difficult to obtain transverse views of the plant phloem sieve plate pores, which are short tubes, to estimate their number and diameters, a method based on longitudinal views is proposed. This method uses recent methods to estimate the number and the sizes of approximately circular objects from their images, given by slices perpendicular to the objects. Moreover, because such longitudinal views are obtained from slices that are rather close to the plate centres whereas the pore size may vary with the pore distance from the plate edge, a sieve plate reconstruction model was developed and incorporated in the method to consider this bias. The method was successfully tested with published longitudinal views of phloem of Soybean and an exceptional entire transverse view from the same tissue. The method was also validated with simulated slices in two sieve plates from Cucurbita and Phaseolus. This method will likely be useful to estimate and to model the hydraulic conductivity and the architecture of the plant phloem, and it could have applications for other materials with approximately cylindrical structures. Nature Publishing Group 2014-05-12 /pmc/articles/PMC5381363/ /pubmed/24816291 http://dx.doi.org/10.1038/srep04929 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Article
Bussières, Philippe
Estimating the number and size of phloem sieve plate pores using longitudinal views and geometric reconstruction
title Estimating the number and size of phloem sieve plate pores using longitudinal views and geometric reconstruction
title_full Estimating the number and size of phloem sieve plate pores using longitudinal views and geometric reconstruction
title_fullStr Estimating the number and size of phloem sieve plate pores using longitudinal views and geometric reconstruction
title_full_unstemmed Estimating the number and size of phloem sieve plate pores using longitudinal views and geometric reconstruction
title_short Estimating the number and size of phloem sieve plate pores using longitudinal views and geometric reconstruction
title_sort estimating the number and size of phloem sieve plate pores using longitudinal views and geometric reconstruction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381363/
https://www.ncbi.nlm.nih.gov/pubmed/24816291
http://dx.doi.org/10.1038/srep04929
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