Dramatic improvement in genome assembly achieved using doubled-haploid genomes

Improvement in de novo assembly of large genomes is still to be desired. Here, we improved draft genome sequence quality by employing doubled-haploid individuals. We sequenced wildtype and doubled-haploid Takifugu rubripes genomes, under the same conditions, using the Illumina platform and assembled...

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Detalles Bibliográficos
Autores principales: Zhang, Hong, Tan, Engkong, Suzuki, Yutaka, Hirose, Yusuke, Kinoshita, Shigeharu, Okano, Hideyuki, Kudoh, Jun, Shimizu, Atsushi, Saito, Kazuyoshi, Watabe, Shugo, Asakawa, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381364/
https://www.ncbi.nlm.nih.gov/pubmed/25345569
http://dx.doi.org/10.1038/srep06780
Descripción
Sumario:Improvement in de novo assembly of large genomes is still to be desired. Here, we improved draft genome sequence quality by employing doubled-haploid individuals. We sequenced wildtype and doubled-haploid Takifugu rubripes genomes, under the same conditions, using the Illumina platform and assembled contigs with SOAPdenovo2. We observed 5.4-fold and 2.6-fold improvement in the sizes of the N50 contig and scaffold of doubled-haploid individuals, respectively, compared to the wildtype, indicating that the use of a doubled-haploid genome aids in accurate genome analysis.

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