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Evolution and Expression of Tissue Globins in Ray-Finned Fishes
The globin gene family encodes oxygen-binding hemeproteins conserved across the major branches of multicellular life. The origins and evolutionary histories of complete globin repertoires have been established for many vertebrates, but there remain major knowledge gaps for ray-finned fish. Therefore...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Oxford University Press
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381549/ https://www.ncbi.nlm.nih.gov/pubmed/28173090 http://dx.doi.org/10.1093/gbe/evw266 |
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author | Gallagher, Michael D. Macqueen, Daniel J. |
author_facet | Gallagher, Michael D. Macqueen, Daniel J. |
author_sort | Gallagher, Michael D. |
collection | PubMed |
description | The globin gene family encodes oxygen-binding hemeproteins conserved across the major branches of multicellular life. The origins and evolutionary histories of complete globin repertoires have been established for many vertebrates, but there remain major knowledge gaps for ray-finned fish. Therefore, we used phylogenetic, comparative genomic and gene expression analyses to discover and characterize canonical “non-blood” globin family members (i.e., myoglobin, cytoglobin, neuroglobin, globin-X, and globin-Y) across multiple ray-finned fish lineages, revealing novel gene duplicates (paralogs) conserved from whole genome duplication (WGD) and small-scale duplication events. Our key findings were that: (1) globin-X paralogs in teleosts have been retained from the teleost-specific WGD, (2) functional paralogs of cytoglobin, neuroglobin, and globin-X, but not myoglobin, have been conserved from the salmonid-specific WGD, (3) triplicate lineage-specific myoglobin paralogs are conserved in arowanas (Osteoglossiformes), which arose by tandem duplication and diverged under positive selection, (4) globin-Y is retained in multiple early branching fish lineages that diverged before teleosts, and (5) marked variation in tissue-specific expression of globin gene repertoires exists across ray-finned fish evolution, including several previously uncharacterized sites of expression. In this respect, our data provide an interesting link between myoglobin expression and the evolution of air breathing in teleosts. Together, our findings demonstrate great-unrecognized diversity in the repertoire and expression of nonblood globins that has arisen during ray-finned fish evolution. |
format | Online Article Text |
id | pubmed-5381549 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53815492017-04-10 Evolution and Expression of Tissue Globins in Ray-Finned Fishes Gallagher, Michael D. Macqueen, Daniel J. Genome Biol Evol Research Article The globin gene family encodes oxygen-binding hemeproteins conserved across the major branches of multicellular life. The origins and evolutionary histories of complete globin repertoires have been established for many vertebrates, but there remain major knowledge gaps for ray-finned fish. Therefore, we used phylogenetic, comparative genomic and gene expression analyses to discover and characterize canonical “non-blood” globin family members (i.e., myoglobin, cytoglobin, neuroglobin, globin-X, and globin-Y) across multiple ray-finned fish lineages, revealing novel gene duplicates (paralogs) conserved from whole genome duplication (WGD) and small-scale duplication events. Our key findings were that: (1) globin-X paralogs in teleosts have been retained from the teleost-specific WGD, (2) functional paralogs of cytoglobin, neuroglobin, and globin-X, but not myoglobin, have been conserved from the salmonid-specific WGD, (3) triplicate lineage-specific myoglobin paralogs are conserved in arowanas (Osteoglossiformes), which arose by tandem duplication and diverged under positive selection, (4) globin-Y is retained in multiple early branching fish lineages that diverged before teleosts, and (5) marked variation in tissue-specific expression of globin gene repertoires exists across ray-finned fish evolution, including several previously uncharacterized sites of expression. In this respect, our data provide an interesting link between myoglobin expression and the evolution of air breathing in teleosts. Together, our findings demonstrate great-unrecognized diversity in the repertoire and expression of nonblood globins that has arisen during ray-finned fish evolution. Oxford University Press 2016-11-09 /pmc/articles/PMC5381549/ /pubmed/28173090 http://dx.doi.org/10.1093/gbe/evw266 Text en © The Author(s) 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gallagher, Michael D. Macqueen, Daniel J. Evolution and Expression of Tissue Globins in Ray-Finned Fishes |
title | Evolution and Expression of Tissue Globins in Ray-Finned Fishes |
title_full | Evolution and Expression of Tissue Globins in Ray-Finned Fishes |
title_fullStr | Evolution and Expression of Tissue Globins in Ray-Finned Fishes |
title_full_unstemmed | Evolution and Expression of Tissue Globins in Ray-Finned Fishes |
title_short | Evolution and Expression of Tissue Globins in Ray-Finned Fishes |
title_sort | evolution and expression of tissue globins in ray-finned fishes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381549/ https://www.ncbi.nlm.nih.gov/pubmed/28173090 http://dx.doi.org/10.1093/gbe/evw266 |
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