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An Annotated Draft Genome for Radix auricularia (Gastropoda, Mollusca)

Molluscs are the second most species-rich phylum in the animal kingdom, yet only 11 genomes of this group have been published so far. Here, we present the draft genome sequence of the pulmonate freshwater snail Radix auricularia. Six whole genome shotgun libraries with different layouts were sequenc...

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Autores principales: Schell, Tilman, Feldmeyer, Barbara, Schmidt, Hanno, Greshake, Bastian, Tills, Oliver, Truebano, Manuela, Rundle, Simon D., Paule, Juraj, Ebersberger, Ingo, Pfenninger, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381561/
https://www.ncbi.nlm.nih.gov/pubmed/28204581
http://dx.doi.org/10.1093/gbe/evx032
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author Schell, Tilman
Feldmeyer, Barbara
Schmidt, Hanno
Greshake, Bastian
Tills, Oliver
Truebano, Manuela
Rundle, Simon D.
Paule, Juraj
Ebersberger, Ingo
Pfenninger, Markus
author_facet Schell, Tilman
Feldmeyer, Barbara
Schmidt, Hanno
Greshake, Bastian
Tills, Oliver
Truebano, Manuela
Rundle, Simon D.
Paule, Juraj
Ebersberger, Ingo
Pfenninger, Markus
author_sort Schell, Tilman
collection PubMed
description Molluscs are the second most species-rich phylum in the animal kingdom, yet only 11 genomes of this group have been published so far. Here, we present the draft genome sequence of the pulmonate freshwater snail Radix auricularia. Six whole genome shotgun libraries with different layouts were sequenced. The resulting assembly comprises 4,823 scaffolds with a cumulative length of 910 Mb and an overall read coverage of 72×. The assembly contains 94.6% of a metazoan core gene collection, indicating an almost complete coverage of the coding fraction. The discrepancy of ∼690 Mb compared with the estimated genome size of R. auricularia (1.6 Gb) results from a high repeat content of 70% mainly comprising DNA transposons. The annotation of 17,338 protein coding genes was supported by the use of publicly available transcriptome data. This draft will serve as starting point for further genomic and population genetic research in this scientifically important phylum.
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spelling pubmed-53815612017-11-07 An Annotated Draft Genome for Radix auricularia (Gastropoda, Mollusca) Schell, Tilman Feldmeyer, Barbara Schmidt, Hanno Greshake, Bastian Tills, Oliver Truebano, Manuela Rundle, Simon D. Paule, Juraj Ebersberger, Ingo Pfenninger, Markus Genome Biol Evol Genome Report Molluscs are the second most species-rich phylum in the animal kingdom, yet only 11 genomes of this group have been published so far. Here, we present the draft genome sequence of the pulmonate freshwater snail Radix auricularia. Six whole genome shotgun libraries with different layouts were sequenced. The resulting assembly comprises 4,823 scaffolds with a cumulative length of 910 Mb and an overall read coverage of 72×. The assembly contains 94.6% of a metazoan core gene collection, indicating an almost complete coverage of the coding fraction. The discrepancy of ∼690 Mb compared with the estimated genome size of R. auricularia (1.6 Gb) results from a high repeat content of 70% mainly comprising DNA transposons. The annotation of 17,338 protein coding genes was supported by the use of publicly available transcriptome data. This draft will serve as starting point for further genomic and population genetic research in this scientifically important phylum. Oxford University Press 2017-03-01 /pmc/articles/PMC5381561/ /pubmed/28204581 http://dx.doi.org/10.1093/gbe/evx032 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Genome Report
Schell, Tilman
Feldmeyer, Barbara
Schmidt, Hanno
Greshake, Bastian
Tills, Oliver
Truebano, Manuela
Rundle, Simon D.
Paule, Juraj
Ebersberger, Ingo
Pfenninger, Markus
An Annotated Draft Genome for Radix auricularia (Gastropoda, Mollusca)
title An Annotated Draft Genome for Radix auricularia (Gastropoda, Mollusca)
title_full An Annotated Draft Genome for Radix auricularia (Gastropoda, Mollusca)
title_fullStr An Annotated Draft Genome for Radix auricularia (Gastropoda, Mollusca)
title_full_unstemmed An Annotated Draft Genome for Radix auricularia (Gastropoda, Mollusca)
title_short An Annotated Draft Genome for Radix auricularia (Gastropoda, Mollusca)
title_sort annotated draft genome for radix auricularia (gastropoda, mollusca)
topic Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381561/
https://www.ncbi.nlm.nih.gov/pubmed/28204581
http://dx.doi.org/10.1093/gbe/evx032
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