Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture

Bioprocesses capable of producing large scales of resveratrol at nutraceutical grade are in demand. This study herein investigated treatment strategies to induce the production of resveratrol in Vitis vinifera L. cell suspension cultures. Among seven investigated elicitors, jasmonic acid (JA), salic...

Descripción completa

Detalles Bibliográficos
Autores principales: Vuong, Thu V., Franco, Chris, Zhang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381694/
https://www.ncbi.nlm.nih.gov/pubmed/28435798
http://dx.doi.org/10.1016/j.btre.2014.04.002
_version_ 1782519977839951872
author Vuong, Thu V.
Franco, Chris
Zhang, Wei
author_facet Vuong, Thu V.
Franco, Chris
Zhang, Wei
author_sort Vuong, Thu V.
collection PubMed
description Bioprocesses capable of producing large scales of resveratrol at nutraceutical grade are in demand. This study herein investigated treatment strategies to induce the production of resveratrol in Vitis vinifera L. cell suspension cultures. Among seven investigated elicitors, jasmonic acid (JA), salicylic acid, β-glucan (GLU), and chitosan enhanced the production of intracellular resveratrol manyfold. The combined treatment of JA and GLU increased extracellular resveratrol production by up to tenfold. The application of Amberlite XAD-7 resin for in situ removal and artificial storage of secreted resveratrol further increased resveratrol production by up to four orders of magnitude. The level of resveratrol produced in response to the combined treatment with 200 g/L XAD-7, 10 μM JA and 1 mg/mL GLU was approximately 2400 mg/L, allowing the production of resveratrol at an industrial scale. The high yield of resveratrol is due to the involvement of a number of mechanisms working in concert.
format Online
Article
Text
id pubmed-5381694
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-53816942017-04-21 Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture Vuong, Thu V. Franco, Chris Zhang, Wei Biotechnol Rep (Amst) Article Bioprocesses capable of producing large scales of resveratrol at nutraceutical grade are in demand. This study herein investigated treatment strategies to induce the production of resveratrol in Vitis vinifera L. cell suspension cultures. Among seven investigated elicitors, jasmonic acid (JA), salicylic acid, β-glucan (GLU), and chitosan enhanced the production of intracellular resveratrol manyfold. The combined treatment of JA and GLU increased extracellular resveratrol production by up to tenfold. The application of Amberlite XAD-7 resin for in situ removal and artificial storage of secreted resveratrol further increased resveratrol production by up to four orders of magnitude. The level of resveratrol produced in response to the combined treatment with 200 g/L XAD-7, 10 μM JA and 1 mg/mL GLU was approximately 2400 mg/L, allowing the production of resveratrol at an industrial scale. The high yield of resveratrol is due to the involvement of a number of mechanisms working in concert. Elsevier 2014-05-19 /pmc/articles/PMC5381694/ /pubmed/28435798 http://dx.doi.org/10.1016/j.btre.2014.04.002 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Vuong, Thu V.
Franco, Chris
Zhang, Wei
Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture
title Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture
title_full Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture
title_fullStr Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture
title_full_unstemmed Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture
title_short Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture
title_sort treatment strategies for high resveratrol induction in vitis vinifera l. cell suspension culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381694/
https://www.ncbi.nlm.nih.gov/pubmed/28435798
http://dx.doi.org/10.1016/j.btre.2014.04.002
work_keys_str_mv AT vuongthuv treatmentstrategiesforhighresveratrolinductioninvitisviniferalcellsuspensionculture
AT francochris treatmentstrategiesforhighresveratrolinductioninvitisviniferalcellsuspensionculture
AT zhangwei treatmentstrategiesforhighresveratrolinductioninvitisviniferalcellsuspensionculture