Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture
Bioprocesses capable of producing large scales of resveratrol at nutraceutical grade are in demand. This study herein investigated treatment strategies to induce the production of resveratrol in Vitis vinifera L. cell suspension cultures. Among seven investigated elicitors, jasmonic acid (JA), salic...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381694/ https://www.ncbi.nlm.nih.gov/pubmed/28435798 http://dx.doi.org/10.1016/j.btre.2014.04.002 |
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author | Vuong, Thu V. Franco, Chris Zhang, Wei |
author_facet | Vuong, Thu V. Franco, Chris Zhang, Wei |
author_sort | Vuong, Thu V. |
collection | PubMed |
description | Bioprocesses capable of producing large scales of resveratrol at nutraceutical grade are in demand. This study herein investigated treatment strategies to induce the production of resveratrol in Vitis vinifera L. cell suspension cultures. Among seven investigated elicitors, jasmonic acid (JA), salicylic acid, β-glucan (GLU), and chitosan enhanced the production of intracellular resveratrol manyfold. The combined treatment of JA and GLU increased extracellular resveratrol production by up to tenfold. The application of Amberlite XAD-7 resin for in situ removal and artificial storage of secreted resveratrol further increased resveratrol production by up to four orders of magnitude. The level of resveratrol produced in response to the combined treatment with 200 g/L XAD-7, 10 μM JA and 1 mg/mL GLU was approximately 2400 mg/L, allowing the production of resveratrol at an industrial scale. The high yield of resveratrol is due to the involvement of a number of mechanisms working in concert. |
format | Online Article Text |
id | pubmed-5381694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-53816942017-04-21 Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture Vuong, Thu V. Franco, Chris Zhang, Wei Biotechnol Rep (Amst) Article Bioprocesses capable of producing large scales of resveratrol at nutraceutical grade are in demand. This study herein investigated treatment strategies to induce the production of resveratrol in Vitis vinifera L. cell suspension cultures. Among seven investigated elicitors, jasmonic acid (JA), salicylic acid, β-glucan (GLU), and chitosan enhanced the production of intracellular resveratrol manyfold. The combined treatment of JA and GLU increased extracellular resveratrol production by up to tenfold. The application of Amberlite XAD-7 resin for in situ removal and artificial storage of secreted resveratrol further increased resveratrol production by up to four orders of magnitude. The level of resveratrol produced in response to the combined treatment with 200 g/L XAD-7, 10 μM JA and 1 mg/mL GLU was approximately 2400 mg/L, allowing the production of resveratrol at an industrial scale. The high yield of resveratrol is due to the involvement of a number of mechanisms working in concert. Elsevier 2014-05-19 /pmc/articles/PMC5381694/ /pubmed/28435798 http://dx.doi.org/10.1016/j.btre.2014.04.002 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Vuong, Thu V. Franco, Chris Zhang, Wei Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture |
title | Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture |
title_full | Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture |
title_fullStr | Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture |
title_full_unstemmed | Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture |
title_short | Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture |
title_sort | treatment strategies for high resveratrol induction in vitis vinifera l. cell suspension culture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381694/ https://www.ncbi.nlm.nih.gov/pubmed/28435798 http://dx.doi.org/10.1016/j.btre.2014.04.002 |
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