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Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis)

Aquaporin 1 (AQP1) is a member of the transmembrane water channel family of proteins with special structural features, and two AQP1 paralogous genes (aqp1aa and aqp1ab) are reported in teleosts. In the present study, the aqp1aa gene of half-smooth tongue sole (Cynoglossus semilaevis) was cloned and...

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Autores principales: Guo, Hua, Wei, Min, Liu, Yang, Zhu, Ying, Xu, Wenteng, Meng, Liang, Wang, Na, Shao, Changwei, Lu, Sheng, Gao, Fengtao, Cui, Zhongkai, Wei, Zhanfei, Zhao, Fazhen, Chen, Songlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381947/
https://www.ncbi.nlm.nih.gov/pubmed/28380032
http://dx.doi.org/10.1371/journal.pone.0175033
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author Guo, Hua
Wei, Min
Liu, Yang
Zhu, Ying
Xu, Wenteng
Meng, Liang
Wang, Na
Shao, Changwei
Lu, Sheng
Gao, Fengtao
Cui, Zhongkai
Wei, Zhanfei
Zhao, Fazhen
Chen, Songlin
author_facet Guo, Hua
Wei, Min
Liu, Yang
Zhu, Ying
Xu, Wenteng
Meng, Liang
Wang, Na
Shao, Changwei
Lu, Sheng
Gao, Fengtao
Cui, Zhongkai
Wei, Zhanfei
Zhao, Fazhen
Chen, Songlin
author_sort Guo, Hua
collection PubMed
description Aquaporin 1 (AQP1) is a member of the transmembrane water channel family of proteins with special structural features, and two AQP1 paralogous genes (aqp1aa and aqp1ab) are reported in teleosts. In the present study, the aqp1aa gene of half-smooth tongue sole (Cynoglossus semilaevis) was cloned and characterized. The full-length cDNA of aqp1aa is 1411 bp with a 786 bp open reading frame encoding a 261-amino acid putative protein with a characteristic structure consisting of 6 membrane-spanning α-helical domains and two highly conserved asparagine—proline—alanine motifs. Real-time quantitative PCR revealed that aqp1aa mRNA is expressed predominantly in the testis of males and pseudo-males, while its expression is low in the ovary and lowest in doublesex and mab-3-related transcription factor 1(DMRT1) knock out fish and triploid males. In situ hybridization indicated that aqp1aa mRNA is expressed mainly in the germ cells of males and pseudo-males, especially in spermatozoa and spermatids. These results suggest that the aqp1aa may play a role in spermatogenesis of C. semilaevis.
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spelling pubmed-53819472017-04-19 Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis) Guo, Hua Wei, Min Liu, Yang Zhu, Ying Xu, Wenteng Meng, Liang Wang, Na Shao, Changwei Lu, Sheng Gao, Fengtao Cui, Zhongkai Wei, Zhanfei Zhao, Fazhen Chen, Songlin PLoS One Research Article Aquaporin 1 (AQP1) is a member of the transmembrane water channel family of proteins with special structural features, and two AQP1 paralogous genes (aqp1aa and aqp1ab) are reported in teleosts. In the present study, the aqp1aa gene of half-smooth tongue sole (Cynoglossus semilaevis) was cloned and characterized. The full-length cDNA of aqp1aa is 1411 bp with a 786 bp open reading frame encoding a 261-amino acid putative protein with a characteristic structure consisting of 6 membrane-spanning α-helical domains and two highly conserved asparagine—proline—alanine motifs. Real-time quantitative PCR revealed that aqp1aa mRNA is expressed predominantly in the testis of males and pseudo-males, while its expression is low in the ovary and lowest in doublesex and mab-3-related transcription factor 1(DMRT1) knock out fish and triploid males. In situ hybridization indicated that aqp1aa mRNA is expressed mainly in the germ cells of males and pseudo-males, especially in spermatozoa and spermatids. These results suggest that the aqp1aa may play a role in spermatogenesis of C. semilaevis. Public Library of Science 2017-04-05 /pmc/articles/PMC5381947/ /pubmed/28380032 http://dx.doi.org/10.1371/journal.pone.0175033 Text en © 2017 Guo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Guo, Hua
Wei, Min
Liu, Yang
Zhu, Ying
Xu, Wenteng
Meng, Liang
Wang, Na
Shao, Changwei
Lu, Sheng
Gao, Fengtao
Cui, Zhongkai
Wei, Zhanfei
Zhao, Fazhen
Chen, Songlin
Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis)
title Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis)
title_full Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis)
title_fullStr Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis)
title_full_unstemmed Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis)
title_short Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis)
title_sort molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (cynoglossus semilaevis)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381947/
https://www.ncbi.nlm.nih.gov/pubmed/28380032
http://dx.doi.org/10.1371/journal.pone.0175033
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