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Searching for active mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities

The most common type of coniferous mobile genetic elements are retrotransposons. Despite of their early positive impact on evolution of modern coniferous species they can have a significant negative impact for Forestry and breeding. Breaking genomic structural integrity mobile elements can cause phe...

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Autores principales: Pochtovyy, A.A., Baranov, O.Yu., Rubel', I.E., Razumova, O.A., Padutov, V.E., Khromov, A.V., Makhotenko, A.V., Tkachuk, A.P., Makarov, V.V., Gushchin, V.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382025/
https://www.ncbi.nlm.nih.gov/pubmed/28409117
http://dx.doi.org/10.1016/j.gdata.2017.03.010
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author Pochtovyy, A.A.
Baranov, O.Yu.
Rubel', I.E.
Razumova, O.A.
Padutov, V.E.
Khromov, A.V.
Makhotenko, A.V.
Tkachuk, A.P.
Makarov, V.V.
Gushchin, V.A.
author_facet Pochtovyy, A.A.
Baranov, O.Yu.
Rubel', I.E.
Razumova, O.A.
Padutov, V.E.
Khromov, A.V.
Makhotenko, A.V.
Tkachuk, A.P.
Makarov, V.V.
Gushchin, V.A.
author_sort Pochtovyy, A.A.
collection PubMed
description The most common type of coniferous mobile genetic elements are retrotransposons. Despite of their early positive impact on evolution of modern coniferous species they can have a significant negative impact for Forestry and breeding. Breaking genomic structural integrity mobile elements can cause phenotypic defects of plants. In this regard, the study of the diversity of coniferous mobile genetic elements is particularly interesting. In the present paper, we describe mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities. In result of assembled contigs analysis by RepeatMasker 70 mobile genetic elements were identified. A 68 of that were retroelements. Most of elements represented by Gypsy (16 contigs) and Copia (48 contigs). In 4 cases retroelements specific to Pinus taeda were identified. In most cases fragments of integrase (24), reverse transcriptase (22) and RNaseH (15) were identified. Results of the study may be of interest for coniferous breeding and genetic specialists. The raw data of these experiments have been deposited at NCBI under the accession number SAMN06185845.
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spelling pubmed-53820252017-04-13 Searching for active mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities Pochtovyy, A.A. Baranov, O.Yu. Rubel', I.E. Razumova, O.A. Padutov, V.E. Khromov, A.V. Makhotenko, A.V. Tkachuk, A.P. Makarov, V.V. Gushchin, V.A. Genom Data Data in Brief Article The most common type of coniferous mobile genetic elements are retrotransposons. Despite of their early positive impact on evolution of modern coniferous species they can have a significant negative impact for Forestry and breeding. Breaking genomic structural integrity mobile elements can cause phenotypic defects of plants. In this regard, the study of the diversity of coniferous mobile genetic elements is particularly interesting. In the present paper, we describe mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities. In result of assembled contigs analysis by RepeatMasker 70 mobile genetic elements were identified. A 68 of that were retroelements. Most of elements represented by Gypsy (16 contigs) and Copia (48 contigs). In 4 cases retroelements specific to Pinus taeda were identified. In most cases fragments of integrase (24), reverse transcriptase (22) and RNaseH (15) were identified. Results of the study may be of interest for coniferous breeding and genetic specialists. The raw data of these experiments have been deposited at NCBI under the accession number SAMN06185845. Elsevier 2017-03-28 /pmc/articles/PMC5382025/ /pubmed/28409117 http://dx.doi.org/10.1016/j.gdata.2017.03.010 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Data in Brief Article
Pochtovyy, A.A.
Baranov, O.Yu.
Rubel', I.E.
Razumova, O.A.
Padutov, V.E.
Khromov, A.V.
Makhotenko, A.V.
Tkachuk, A.P.
Makarov, V.V.
Gushchin, V.A.
Searching for active mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities
title Searching for active mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities
title_full Searching for active mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities
title_fullStr Searching for active mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities
title_full_unstemmed Searching for active mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities
title_short Searching for active mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities
title_sort searching for active mobile genetic elements in dsrna fraction of pinus sylvestris having witches broom abnormalities
topic Data in Brief Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382025/
https://www.ncbi.nlm.nih.gov/pubmed/28409117
http://dx.doi.org/10.1016/j.gdata.2017.03.010
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