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Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women

BACKGROUND: Rubella is an acute infectious disease caused by Rubella virus (RUBV). RUBV remains an important pathogen worldwide, causing approximately 100 000 cases of congenital rubella syndrome (CRS) every year; and the most severe consequence of rubella is teratogenicity. The aim of this study wa...

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Autores principales: Zanga, Josue, Mbanzulu, Makola Kennedy, Kabasele, Arnold-Freddy, Ngatu, Nlandu Roger, Wumba, Dimosi Roger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382441/
https://www.ncbi.nlm.nih.gov/pubmed/28381214
http://dx.doi.org/10.1186/s12879-017-2352-6
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author Zanga, Josue
Mbanzulu, Makola Kennedy
Kabasele, Arnold-Freddy
Ngatu, Nlandu Roger
Wumba, Dimosi Roger
author_facet Zanga, Josue
Mbanzulu, Makola Kennedy
Kabasele, Arnold-Freddy
Ngatu, Nlandu Roger
Wumba, Dimosi Roger
author_sort Zanga, Josue
collection PubMed
description BACKGROUND: Rubella is an acute infectious disease caused by Rubella virus (RUBV). RUBV remains an important pathogen worldwide, causing approximately 100 000 cases of congenital rubella syndrome (CRS) every year; and the most severe consequence of rubella is teratogenicity. The aim of this study was to estimate the prevalence of RUBV IgG antibodies and determine RUBV genotypes in Congolese pregnant women in Kongo central province, Democratic Republic of Congo (DRC). METHODS: This was a prospective cross-sectional study that consisted of a laboratory analysis of blood samples from 78 pregnant women to check for the presence of RUBV IgG antibodies, and also determine RUBV genotypes in seropositive samples (using primers targeting RUBV nucleoprotein), with the use of serological and molecular methods, respectively. Participants were pregnant women attending antenatal care clinics (ANC) at two health zones of Kisantu town in DRC. They were followed-up from the first to third trimester. Those who were negative for RUBV antibodies at the initial assay (first trimester) were tested in the second and, eventually, the third trimester. RESULTS: An overall rubella seroprevalence of 58.97% was observed, whereas RUBV nucleoprotein was detected in 60% of randomly selected 30 blood samples among the 46 RUBV seropositive pregnant women. Five (27.77%) of positive samples were positive for both RUBV genotypes (RV8633/9112 and RV8945/9577), whereas 11 (61.11%) of them were positive for RV8633/9112 and two (11.11%) were positive for RV8945/9577 only. Regarding rubella clinical signs and complications, two subjects (2.56%) presented with fever, whereas five pregnant women (6.41%) had experienced abortion. None (0%) of the participants has been vaccinated against RUBV. CONCLUSIONS: Findings from this study suggest that RUBV is prevalent in Congolese pregnant women. Further research is required to elucidate the molecular epidemiology of RUBV in order to design a rational rubella surveillance and control program in DRC.
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spelling pubmed-53824412017-04-10 Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women Zanga, Josue Mbanzulu, Makola Kennedy Kabasele, Arnold-Freddy Ngatu, Nlandu Roger Wumba, Dimosi Roger BMC Infect Dis Research Article BACKGROUND: Rubella is an acute infectious disease caused by Rubella virus (RUBV). RUBV remains an important pathogen worldwide, causing approximately 100 000 cases of congenital rubella syndrome (CRS) every year; and the most severe consequence of rubella is teratogenicity. The aim of this study was to estimate the prevalence of RUBV IgG antibodies and determine RUBV genotypes in Congolese pregnant women in Kongo central province, Democratic Republic of Congo (DRC). METHODS: This was a prospective cross-sectional study that consisted of a laboratory analysis of blood samples from 78 pregnant women to check for the presence of RUBV IgG antibodies, and also determine RUBV genotypes in seropositive samples (using primers targeting RUBV nucleoprotein), with the use of serological and molecular methods, respectively. Participants were pregnant women attending antenatal care clinics (ANC) at two health zones of Kisantu town in DRC. They were followed-up from the first to third trimester. Those who were negative for RUBV antibodies at the initial assay (first trimester) were tested in the second and, eventually, the third trimester. RESULTS: An overall rubella seroprevalence of 58.97% was observed, whereas RUBV nucleoprotein was detected in 60% of randomly selected 30 blood samples among the 46 RUBV seropositive pregnant women. Five (27.77%) of positive samples were positive for both RUBV genotypes (RV8633/9112 and RV8945/9577), whereas 11 (61.11%) of them were positive for RV8633/9112 and two (11.11%) were positive for RV8945/9577 only. Regarding rubella clinical signs and complications, two subjects (2.56%) presented with fever, whereas five pregnant women (6.41%) had experienced abortion. None (0%) of the participants has been vaccinated against RUBV. CONCLUSIONS: Findings from this study suggest that RUBV is prevalent in Congolese pregnant women. Further research is required to elucidate the molecular epidemiology of RUBV in order to design a rational rubella surveillance and control program in DRC. BioMed Central 2017-04-05 /pmc/articles/PMC5382441/ /pubmed/28381214 http://dx.doi.org/10.1186/s12879-017-2352-6 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zanga, Josue
Mbanzulu, Makola Kennedy
Kabasele, Arnold-Freddy
Ngatu, Nlandu Roger
Wumba, Dimosi Roger
Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women
title Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women
title_full Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women
title_fullStr Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women
title_full_unstemmed Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women
title_short Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women
title_sort rubella seroprevalence and real-time pcr detection of rubv among congolese pregnant women
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382441/
https://www.ncbi.nlm.nih.gov/pubmed/28381214
http://dx.doi.org/10.1186/s12879-017-2352-6
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