From rabbit antibody repertoires to rabbit monoclonal antibodies

In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in...

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Detalles Bibliográficos
Autores principales: Weber, Justus, Peng, Haiyong, Rader, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382564/
https://www.ncbi.nlm.nih.gov/pubmed/28336958
http://dx.doi.org/10.1038/emm.2017.23
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author Weber, Justus
Peng, Haiyong
Rader, Christoph
author_facet Weber, Justus
Peng, Haiyong
Rader, Christoph
author_sort Weber, Justus
collection PubMed
description In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies.
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spelling pubmed-53825642017-04-07 From rabbit antibody repertoires to rabbit monoclonal antibodies Weber, Justus Peng, Haiyong Rader, Christoph Exp Mol Med Review In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies. Nature Publishing Group 2017-03 2017-03-24 /pmc/articles/PMC5382564/ /pubmed/28336958 http://dx.doi.org/10.1038/emm.2017.23 Text en Copyright © 2017 The Author(s) http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Review
Weber, Justus
Peng, Haiyong
Rader, Christoph
From rabbit antibody repertoires to rabbit monoclonal antibodies
title From rabbit antibody repertoires to rabbit monoclonal antibodies
title_full From rabbit antibody repertoires to rabbit monoclonal antibodies
title_fullStr From rabbit antibody repertoires to rabbit monoclonal antibodies
title_full_unstemmed From rabbit antibody repertoires to rabbit monoclonal antibodies
title_short From rabbit antibody repertoires to rabbit monoclonal antibodies
title_sort from rabbit antibody repertoires to rabbit monoclonal antibodies
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382564/
https://www.ncbi.nlm.nih.gov/pubmed/28336958
http://dx.doi.org/10.1038/emm.2017.23
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