Cargando…

Towards the complete proteinaceous regulome of Acinetobacter baumannii

The emergence of Acinetobacter baumannii strains, with broad multidrug-resistance phenotypes and novel virulence factors unique to hypervirulent strains, presents a major threat to human health worldwide. Although a number of studies have described virulence-affecting entities for this organism, ver...

Descripción completa

Detalles Bibliográficos
Autores principales: Casella, Leila G, Weiss, Andy, Pérez-Rueda, Ernesto, Antonio Ibarra, J, Shaw, Lindsey N
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382811/
https://www.ncbi.nlm.nih.gov/pubmed/28663824
http://dx.doi.org/10.1099/mgen.0.000107
_version_ 1782520170372136960
author Casella, Leila G
Weiss, Andy
Pérez-Rueda, Ernesto
Antonio Ibarra, J
Shaw, Lindsey N
author_facet Casella, Leila G
Weiss, Andy
Pérez-Rueda, Ernesto
Antonio Ibarra, J
Shaw, Lindsey N
author_sort Casella, Leila G
collection PubMed
description The emergence of Acinetobacter baumannii strains, with broad multidrug-resistance phenotypes and novel virulence factors unique to hypervirulent strains, presents a major threat to human health worldwide. Although a number of studies have described virulence-affecting entities for this organism, very few have identified regulatory elements controlling their expression. Previously, our group has documented the global identification and curation of regulatory RNAs in A. baumannii. As such, in the present study, we detail an extension of this work, the performance of an extensive bioinformatic analysis to identify regulatory proteins in the recently annotated genome of the highly virulent AB5075 strain. In so doing, 243 transcription factors, 14 two-component systems (TCSs), 2 orphan response regulators, 1 hybrid TCS and 5 σ factors were found. A comparison of these elements between AB5075 and other clinical isolates, as well as a laboratory strain, led to the identification of several conserved regulatory elements, whilst at the same time uncovering regulators unique to hypervirulent strains. Lastly, by comparing regulatory elements compiled in this study to genes shown to be essential for AB5075 infection, we were able to highlight elements with a specific importance for pathogenic behaviour. Collectively, our work offers a unique insight into the regulatory network of A. baumannii strains, and provides insight into the evolution of hypervirulent lineages.
format Online
Article
Text
id pubmed-5382811
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-53828112017-06-29 Towards the complete proteinaceous regulome of Acinetobacter baumannii Casella, Leila G Weiss, Andy Pérez-Rueda, Ernesto Antonio Ibarra, J Shaw, Lindsey N Microb Genom Research Article The emergence of Acinetobacter baumannii strains, with broad multidrug-resistance phenotypes and novel virulence factors unique to hypervirulent strains, presents a major threat to human health worldwide. Although a number of studies have described virulence-affecting entities for this organism, very few have identified regulatory elements controlling their expression. Previously, our group has documented the global identification and curation of regulatory RNAs in A. baumannii. As such, in the present study, we detail an extension of this work, the performance of an extensive bioinformatic analysis to identify regulatory proteins in the recently annotated genome of the highly virulent AB5075 strain. In so doing, 243 transcription factors, 14 two-component systems (TCSs), 2 orphan response regulators, 1 hybrid TCS and 5 σ factors were found. A comparison of these elements between AB5075 and other clinical isolates, as well as a laboratory strain, led to the identification of several conserved regulatory elements, whilst at the same time uncovering regulators unique to hypervirulent strains. Lastly, by comparing regulatory elements compiled in this study to genes shown to be essential for AB5075 infection, we were able to highlight elements with a specific importance for pathogenic behaviour. Collectively, our work offers a unique insight into the regulatory network of A. baumannii strains, and provides insight into the evolution of hypervirulent lineages. Microbiology Society 2017-03-23 /pmc/articles/PMC5382811/ /pubmed/28663824 http://dx.doi.org/10.1099/mgen.0.000107 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Casella, Leila G
Weiss, Andy
Pérez-Rueda, Ernesto
Antonio Ibarra, J
Shaw, Lindsey N
Towards the complete proteinaceous regulome of Acinetobacter baumannii
title Towards the complete proteinaceous regulome of Acinetobacter baumannii
title_full Towards the complete proteinaceous regulome of Acinetobacter baumannii
title_fullStr Towards the complete proteinaceous regulome of Acinetobacter baumannii
title_full_unstemmed Towards the complete proteinaceous regulome of Acinetobacter baumannii
title_short Towards the complete proteinaceous regulome of Acinetobacter baumannii
title_sort towards the complete proteinaceous regulome of acinetobacter baumannii
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382811/
https://www.ncbi.nlm.nih.gov/pubmed/28663824
http://dx.doi.org/10.1099/mgen.0.000107
work_keys_str_mv AT casellaleilag towardsthecompleteproteinaceousregulomeofacinetobacterbaumannii
AT weissandy towardsthecompleteproteinaceousregulomeofacinetobacterbaumannii
AT perezruedaernesto towardsthecompleteproteinaceousregulomeofacinetobacterbaumannii
AT antonioibarraj towardsthecompleteproteinaceousregulomeofacinetobacterbaumannii
AT shawlindseyn towardsthecompleteproteinaceousregulomeofacinetobacterbaumannii