Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy

The changes of the morphology during heating and the degree of crystallinity of dry and hydrated starch granules are investigated using second harmonic generation (SHG) based Stokes polarimetry. A spatial distribution of various polarization parameters, such as the degree of polarization (DOP), the...

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Autores principales: Mazumder, Nirmal, Xiang, Lu Yun, Qiu, Jianjun, Kao, Fu-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382894/
https://www.ncbi.nlm.nih.gov/pubmed/28383522
http://dx.doi.org/10.1038/srep45816
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author Mazumder, Nirmal
Xiang, Lu Yun
Qiu, Jianjun
Kao, Fu-Jen
author_facet Mazumder, Nirmal
Xiang, Lu Yun
Qiu, Jianjun
Kao, Fu-Jen
author_sort Mazumder, Nirmal
collection PubMed
description The changes of the morphology during heating and the degree of crystallinity of dry and hydrated starch granules are investigated using second harmonic generation (SHG) based Stokes polarimetry. A spatial distribution of various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP) are extracted and compared with the two dimensional second harmonic (SH) Stokes images of starch granules. The SH signal from hydrated and dry starch on heating differed significantly in DOLP and DOCP values, indicating that hydrated starch has a greater degree of ultrastructural amylopectin disorder. The detail of denaturation and the phase transition of hydrated starch demonstrate the significant influence of thermal processing.
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spelling pubmed-53828942017-04-11 Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy Mazumder, Nirmal Xiang, Lu Yun Qiu, Jianjun Kao, Fu-Jen Sci Rep Article The changes of the morphology during heating and the degree of crystallinity of dry and hydrated starch granules are investigated using second harmonic generation (SHG) based Stokes polarimetry. A spatial distribution of various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP) are extracted and compared with the two dimensional second harmonic (SH) Stokes images of starch granules. The SH signal from hydrated and dry starch on heating differed significantly in DOLP and DOCP values, indicating that hydrated starch has a greater degree of ultrastructural amylopectin disorder. The detail of denaturation and the phase transition of hydrated starch demonstrate the significant influence of thermal processing. Nature Publishing Group 2017-04-06 /pmc/articles/PMC5382894/ /pubmed/28383522 http://dx.doi.org/10.1038/srep45816 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mazumder, Nirmal
Xiang, Lu Yun
Qiu, Jianjun
Kao, Fu-Jen
Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy
title Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy
title_full Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy
title_fullStr Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy
title_full_unstemmed Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy
title_short Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy
title_sort investigating starch gelatinization through stokes vector resolved second harmonic generation microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382894/
https://www.ncbi.nlm.nih.gov/pubmed/28383522
http://dx.doi.org/10.1038/srep45816
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