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The effect of the serum corona on interactions between a single nano-object and a living cell

Nanoparticles (NPs) which enter physiological fluids are rapidly coated by proteins, forming a so-called corona which may strongly modify their interaction with tissues and cells relative to the bare NPs. In this work the interactions between a living cell and a nano-object, and in particular the ef...

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Autores principales: Dror, Yael, Sorkin, Raya, Brand, Guy, Boubriak, Olga, Urban, Jill, Klein, Jacob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382918/
https://www.ncbi.nlm.nih.gov/pubmed/28383528
http://dx.doi.org/10.1038/srep45758
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author Dror, Yael
Sorkin, Raya
Brand, Guy
Boubriak, Olga
Urban, Jill
Klein, Jacob
author_facet Dror, Yael
Sorkin, Raya
Brand, Guy
Boubriak, Olga
Urban, Jill
Klein, Jacob
author_sort Dror, Yael
collection PubMed
description Nanoparticles (NPs) which enter physiological fluids are rapidly coated by proteins, forming a so-called corona which may strongly modify their interaction with tissues and cells relative to the bare NPs. In this work the interactions between a living cell and a nano-object, and in particular the effect on this of the adsorption of serum proteins, are directly examined by measuring the forces arising as an Atomic Force Microscope tip (diameter 20 nm) - simulating a nano-object - approaches and contacts a cell. We find that the presence of a serum protein corona on the tip strongly modifies the interaction as indicated by pronounced increase in the indentation, hysteresis and work of adhesion compared to a bare tip. Classically one expects an AFM tip interacting with a cell surface to be repelled due to cell elastic distortion, offset by tip-cell adhesion, and indeed such a model fits the bare-tip/cell interaction, in agreement with earlier work. However, the force plots obtained with serum-modified tips are very different, indicating that the cell is much more compliant to the approaching tip. The insights obtained in this work may promote better design of NPs for drug delivery and other nano-medical applications.
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spelling pubmed-53829182017-04-11 The effect of the serum corona on interactions between a single nano-object and a living cell Dror, Yael Sorkin, Raya Brand, Guy Boubriak, Olga Urban, Jill Klein, Jacob Sci Rep Article Nanoparticles (NPs) which enter physiological fluids are rapidly coated by proteins, forming a so-called corona which may strongly modify their interaction with tissues and cells relative to the bare NPs. In this work the interactions between a living cell and a nano-object, and in particular the effect on this of the adsorption of serum proteins, are directly examined by measuring the forces arising as an Atomic Force Microscope tip (diameter 20 nm) - simulating a nano-object - approaches and contacts a cell. We find that the presence of a serum protein corona on the tip strongly modifies the interaction as indicated by pronounced increase in the indentation, hysteresis and work of adhesion compared to a bare tip. Classically one expects an AFM tip interacting with a cell surface to be repelled due to cell elastic distortion, offset by tip-cell adhesion, and indeed such a model fits the bare-tip/cell interaction, in agreement with earlier work. However, the force plots obtained with serum-modified tips are very different, indicating that the cell is much more compliant to the approaching tip. The insights obtained in this work may promote better design of NPs for drug delivery and other nano-medical applications. Nature Publishing Group 2017-04-06 /pmc/articles/PMC5382918/ /pubmed/28383528 http://dx.doi.org/10.1038/srep45758 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Dror, Yael
Sorkin, Raya
Brand, Guy
Boubriak, Olga
Urban, Jill
Klein, Jacob
The effect of the serum corona on interactions between a single nano-object and a living cell
title The effect of the serum corona on interactions between a single nano-object and a living cell
title_full The effect of the serum corona on interactions between a single nano-object and a living cell
title_fullStr The effect of the serum corona on interactions between a single nano-object and a living cell
title_full_unstemmed The effect of the serum corona on interactions between a single nano-object and a living cell
title_short The effect of the serum corona on interactions between a single nano-object and a living cell
title_sort effect of the serum corona on interactions between a single nano-object and a living cell
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382918/
https://www.ncbi.nlm.nih.gov/pubmed/28383528
http://dx.doi.org/10.1038/srep45758
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