Cargando…
Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering
Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells that have been successfully used in human bone tissue engineering. To establish whether these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with t...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383003/ https://www.ncbi.nlm.nih.gov/pubmed/28209631 http://dx.doi.org/10.1042/CS20170047 |
_version_ | 1782520200934981632 |
---|---|
author | Paino, Francesca La Noce, Marcella Giuliani, Alessandra De Rosa, Alfredo Mazzoni, Serena Laino, Luigi Amler, Evzen Papaccio, Gianpaolo Desiderio, Vincenzo Tirino, Virginia |
author_facet | Paino, Francesca La Noce, Marcella Giuliani, Alessandra De Rosa, Alfredo Mazzoni, Serena Laino, Luigi Amler, Evzen Papaccio, Gianpaolo Desiderio, Vincenzo Tirino, Virginia |
author_sort | Paino, Francesca |
collection | PubMed |
description | Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells that have been successfully used in human bone tissue engineering. To establish whether these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with the specific aim of obtaining a new tool for bone transplantation. Therefore, hDPSCs were specifically selected from the stromal–vascular dental pulp fraction, using appropriate markers, and cultured. Growth curves, expression of bone-related markers, calcification and angiogenesis as well as an in vivo transplantation assay were performed. We found that hDPSCs proliferate, differentiate into osteoblasts and express high levels of angiogenic genes, such as vascular endothelial growth factor and platelet-derived growth factor A. Human DPSCs, after 40 days of culture, give rise to a 3D structure resembling a woven fibrous bone. These woven bone (WB) samples were analysed using classic histology and synchrotron-based, X-ray phase-contrast microtomography and holotomography. WB showed histological and attractive physical qualities of bone with few areas of mineralization and neovessels. Such WB, when transplanted into rats, was remodelled into vascularized bone tissue. Taken together, our data lead to the assumption that WB samples, fabricated by DPSCs, constitute a noteworthy tool and do not need the use of scaffolds, and therefore they are ready for customized regeneration. |
format | Online Article Text |
id | pubmed-5383003 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53830032017-04-21 Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering Paino, Francesca La Noce, Marcella Giuliani, Alessandra De Rosa, Alfredo Mazzoni, Serena Laino, Luigi Amler, Evzen Papaccio, Gianpaolo Desiderio, Vincenzo Tirino, Virginia Clin Sci (Lond) Research Articles Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells that have been successfully used in human bone tissue engineering. To establish whether these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with the specific aim of obtaining a new tool for bone transplantation. Therefore, hDPSCs were specifically selected from the stromal–vascular dental pulp fraction, using appropriate markers, and cultured. Growth curves, expression of bone-related markers, calcification and angiogenesis as well as an in vivo transplantation assay were performed. We found that hDPSCs proliferate, differentiate into osteoblasts and express high levels of angiogenic genes, such as vascular endothelial growth factor and platelet-derived growth factor A. Human DPSCs, after 40 days of culture, give rise to a 3D structure resembling a woven fibrous bone. These woven bone (WB) samples were analysed using classic histology and synchrotron-based, X-ray phase-contrast microtomography and holotomography. WB showed histological and attractive physical qualities of bone with few areas of mineralization and neovessels. Such WB, when transplanted into rats, was remodelled into vascularized bone tissue. Taken together, our data lead to the assumption that WB samples, fabricated by DPSCs, constitute a noteworthy tool and do not need the use of scaffolds, and therefore they are ready for customized regeneration. Portland Press Ltd. 2017-04-06 2017-04-25 /pmc/articles/PMC5383003/ /pubmed/28209631 http://dx.doi.org/10.1042/CS20170047 Text en © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society http://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Research Articles Paino, Francesca La Noce, Marcella Giuliani, Alessandra De Rosa, Alfredo Mazzoni, Serena Laino, Luigi Amler, Evzen Papaccio, Gianpaolo Desiderio, Vincenzo Tirino, Virginia Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering |
title | Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering |
title_full | Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering |
title_fullStr | Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering |
title_full_unstemmed | Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering |
title_short | Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering |
title_sort | human dpscs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383003/ https://www.ncbi.nlm.nih.gov/pubmed/28209631 http://dx.doi.org/10.1042/CS20170047 |
work_keys_str_mv | AT painofrancesca humandpscsfabricatevascularizedwovenbonetissueanewtoolinbonetissueengineering AT lanocemarcella humandpscsfabricatevascularizedwovenbonetissueanewtoolinbonetissueengineering AT giulianialessandra humandpscsfabricatevascularizedwovenbonetissueanewtoolinbonetissueengineering AT derosaalfredo humandpscsfabricatevascularizedwovenbonetissueanewtoolinbonetissueengineering AT mazzoniserena humandpscsfabricatevascularizedwovenbonetissueanewtoolinbonetissueengineering AT lainoluigi humandpscsfabricatevascularizedwovenbonetissueanewtoolinbonetissueengineering AT amlerevzen humandpscsfabricatevascularizedwovenbonetissueanewtoolinbonetissueengineering AT papacciogianpaolo humandpscsfabricatevascularizedwovenbonetissueanewtoolinbonetissueengineering AT desideriovincenzo humandpscsfabricatevascularizedwovenbonetissueanewtoolinbonetissueengineering AT tirinovirginia humandpscsfabricatevascularizedwovenbonetissueanewtoolinbonetissueengineering |