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Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering

Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells that have been successfully used in human bone tissue engineering. To establish whether these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with t...

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Autores principales: Paino, Francesca, La Noce, Marcella, Giuliani, Alessandra, De Rosa, Alfredo, Mazzoni, Serena, Laino, Luigi, Amler, Evzen, Papaccio, Gianpaolo, Desiderio, Vincenzo, Tirino, Virginia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383003/
https://www.ncbi.nlm.nih.gov/pubmed/28209631
http://dx.doi.org/10.1042/CS20170047
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author Paino, Francesca
La Noce, Marcella
Giuliani, Alessandra
De Rosa, Alfredo
Mazzoni, Serena
Laino, Luigi
Amler, Evzen
Papaccio, Gianpaolo
Desiderio, Vincenzo
Tirino, Virginia
author_facet Paino, Francesca
La Noce, Marcella
Giuliani, Alessandra
De Rosa, Alfredo
Mazzoni, Serena
Laino, Luigi
Amler, Evzen
Papaccio, Gianpaolo
Desiderio, Vincenzo
Tirino, Virginia
author_sort Paino, Francesca
collection PubMed
description Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells that have been successfully used in human bone tissue engineering. To establish whether these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with the specific aim of obtaining a new tool for bone transplantation. Therefore, hDPSCs were specifically selected from the stromal–vascular dental pulp fraction, using appropriate markers, and cultured. Growth curves, expression of bone-related markers, calcification and angiogenesis as well as an in vivo transplantation assay were performed. We found that hDPSCs proliferate, differentiate into osteoblasts and express high levels of angiogenic genes, such as vascular endothelial growth factor and platelet-derived growth factor A. Human DPSCs, after 40 days of culture, give rise to a 3D structure resembling a woven fibrous bone. These woven bone (WB) samples were analysed using classic histology and synchrotron-based, X-ray phase-contrast microtomography and holotomography. WB showed histological and attractive physical qualities of bone with few areas of mineralization and neovessels. Such WB, when transplanted into rats, was remodelled into vascularized bone tissue. Taken together, our data lead to the assumption that WB samples, fabricated by DPSCs, constitute a noteworthy tool and do not need the use of scaffolds, and therefore they are ready for customized regeneration.
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spelling pubmed-53830032017-04-21 Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering Paino, Francesca La Noce, Marcella Giuliani, Alessandra De Rosa, Alfredo Mazzoni, Serena Laino, Luigi Amler, Evzen Papaccio, Gianpaolo Desiderio, Vincenzo Tirino, Virginia Clin Sci (Lond) Research Articles Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells that have been successfully used in human bone tissue engineering. To establish whether these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with the specific aim of obtaining a new tool for bone transplantation. Therefore, hDPSCs were specifically selected from the stromal–vascular dental pulp fraction, using appropriate markers, and cultured. Growth curves, expression of bone-related markers, calcification and angiogenesis as well as an in vivo transplantation assay were performed. We found that hDPSCs proliferate, differentiate into osteoblasts and express high levels of angiogenic genes, such as vascular endothelial growth factor and platelet-derived growth factor A. Human DPSCs, after 40 days of culture, give rise to a 3D structure resembling a woven fibrous bone. These woven bone (WB) samples were analysed using classic histology and synchrotron-based, X-ray phase-contrast microtomography and holotomography. WB showed histological and attractive physical qualities of bone with few areas of mineralization and neovessels. Such WB, when transplanted into rats, was remodelled into vascularized bone tissue. Taken together, our data lead to the assumption that WB samples, fabricated by DPSCs, constitute a noteworthy tool and do not need the use of scaffolds, and therefore they are ready for customized regeneration. Portland Press Ltd. 2017-04-06 2017-04-25 /pmc/articles/PMC5383003/ /pubmed/28209631 http://dx.doi.org/10.1042/CS20170047 Text en © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society http://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Research Articles
Paino, Francesca
La Noce, Marcella
Giuliani, Alessandra
De Rosa, Alfredo
Mazzoni, Serena
Laino, Luigi
Amler, Evzen
Papaccio, Gianpaolo
Desiderio, Vincenzo
Tirino, Virginia
Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering
title Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering
title_full Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering
title_fullStr Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering
title_full_unstemmed Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering
title_short Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering
title_sort human dpscs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383003/
https://www.ncbi.nlm.nih.gov/pubmed/28209631
http://dx.doi.org/10.1042/CS20170047
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