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Acute oral dose of sodium nitrite induces redox imbalance, DNA damage, metabolic and histological changes in rat intestine
Industrialization and unchecked use of nitrate/nitrite salts for various purposes has increased human exposure to high levels of sodium nitrite (NaNO(2)) which can act as a pro-oxidant and pro-carcinogen. Oral exposure makes the gastrointestinal tract particularly susceptible to nitrite toxicity. In...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383256/ https://www.ncbi.nlm.nih.gov/pubmed/28384248 http://dx.doi.org/10.1371/journal.pone.0175196 |
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author | Ansari, Fariheen Aisha Ali, Shaikh Nisar Arif, Hussain Khan, Aijaz Ahmed Mahmood, Riaz |
author_facet | Ansari, Fariheen Aisha Ali, Shaikh Nisar Arif, Hussain Khan, Aijaz Ahmed Mahmood, Riaz |
author_sort | Ansari, Fariheen Aisha |
collection | PubMed |
description | Industrialization and unchecked use of nitrate/nitrite salts for various purposes has increased human exposure to high levels of sodium nitrite (NaNO(2)) which can act as a pro-oxidant and pro-carcinogen. Oral exposure makes the gastrointestinal tract particularly susceptible to nitrite toxicity. In this work, the effect of administration of a single acute oral dose of NaNO(2) on rat intestine was studied. Animals were randomly divided into four groups and given single doses of 20, 40, 60 and 75 mg NaNO(2)/kg body weight. Untreated animals served as the control group. An NaNO(2) dose-dependent decline in the activities of brush border membrane enzymes, increase in lipid peroxidation, protein oxidation, hydrogen peroxide levels and decreased thiol content was observed in all treated groups. The activities of various metabolic and antioxidant defense enzymes were also altered. NaNO(2) induced a dose-dependent increase in DNA damage and DNA-protein crosslinking. Histopathological studies showed marked morphological damage in intestinal cells. The intestinal damage might be due to nitrite-induced oxidative stress, direct action of nitrite anion or chemical modification by reaction intermediates. |
format | Online Article Text |
id | pubmed-5383256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53832562017-05-03 Acute oral dose of sodium nitrite induces redox imbalance, DNA damage, metabolic and histological changes in rat intestine Ansari, Fariheen Aisha Ali, Shaikh Nisar Arif, Hussain Khan, Aijaz Ahmed Mahmood, Riaz PLoS One Research Article Industrialization and unchecked use of nitrate/nitrite salts for various purposes has increased human exposure to high levels of sodium nitrite (NaNO(2)) which can act as a pro-oxidant and pro-carcinogen. Oral exposure makes the gastrointestinal tract particularly susceptible to nitrite toxicity. In this work, the effect of administration of a single acute oral dose of NaNO(2) on rat intestine was studied. Animals were randomly divided into four groups and given single doses of 20, 40, 60 and 75 mg NaNO(2)/kg body weight. Untreated animals served as the control group. An NaNO(2) dose-dependent decline in the activities of brush border membrane enzymes, increase in lipid peroxidation, protein oxidation, hydrogen peroxide levels and decreased thiol content was observed in all treated groups. The activities of various metabolic and antioxidant defense enzymes were also altered. NaNO(2) induced a dose-dependent increase in DNA damage and DNA-protein crosslinking. Histopathological studies showed marked morphological damage in intestinal cells. The intestinal damage might be due to nitrite-induced oxidative stress, direct action of nitrite anion or chemical modification by reaction intermediates. Public Library of Science 2017-04-06 /pmc/articles/PMC5383256/ /pubmed/28384248 http://dx.doi.org/10.1371/journal.pone.0175196 Text en © 2017 Ansari et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ansari, Fariheen Aisha Ali, Shaikh Nisar Arif, Hussain Khan, Aijaz Ahmed Mahmood, Riaz Acute oral dose of sodium nitrite induces redox imbalance, DNA damage, metabolic and histological changes in rat intestine |
title | Acute oral dose of sodium nitrite induces redox imbalance, DNA damage, metabolic and histological changes in rat intestine |
title_full | Acute oral dose of sodium nitrite induces redox imbalance, DNA damage, metabolic and histological changes in rat intestine |
title_fullStr | Acute oral dose of sodium nitrite induces redox imbalance, DNA damage, metabolic and histological changes in rat intestine |
title_full_unstemmed | Acute oral dose of sodium nitrite induces redox imbalance, DNA damage, metabolic and histological changes in rat intestine |
title_short | Acute oral dose of sodium nitrite induces redox imbalance, DNA damage, metabolic and histological changes in rat intestine |
title_sort | acute oral dose of sodium nitrite induces redox imbalance, dna damage, metabolic and histological changes in rat intestine |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383256/ https://www.ncbi.nlm.nih.gov/pubmed/28384248 http://dx.doi.org/10.1371/journal.pone.0175196 |
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