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Concurrence in the ability for lipid synthesis between life stages in insects
The ability to synthesize lipids is critical for an organism’s fitness; hence, metabolic pathways, underlying lipid synthesis, tend to be highly conserved. Surprisingly, the majority of parasitoids deviate from this general metabolic model by lacking the ability to convert sugars and other carbohydr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society Publishing
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383825/ https://www.ncbi.nlm.nih.gov/pubmed/28405368 http://dx.doi.org/10.1098/rsos.160815 |
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author | Visser, Bertanne Willett, Denis S. Harvey, Jeffrey A. Alborn, Hans T. |
author_facet | Visser, Bertanne Willett, Denis S. Harvey, Jeffrey A. Alborn, Hans T. |
author_sort | Visser, Bertanne |
collection | PubMed |
description | The ability to synthesize lipids is critical for an organism’s fitness; hence, metabolic pathways, underlying lipid synthesis, tend to be highly conserved. Surprisingly, the majority of parasitoids deviate from this general metabolic model by lacking the ability to convert sugars and other carbohydrates into lipids. These insects spend the first part of their life feeding and developing in or on an arthropod host, during which they can carry over a substantial amount of lipid reserves. While many parasitoid species have been tested for lipogenic ability at the adult life stage, it has remained unclear whether parasitoid larvae can synthesize lipids. Here we investigate whether or not several insects can synthesize lipids during the larval stage using three ectoparasitic wasps (developing on the outside of the host) and the vinegar fly Drosophila melanogaster that differ in lipogenic ability in the adult life stage. Using feeding experiments and stable isotope tracing with gas chromatography/mass spectrometry, we first confirm lipogenic abilities in the adult life stage. Using topical application of stable isotopes in developing larvae, we then provide clear evidence of concurrence in lipogenic ability between larval and adult life stages in all species tested. |
format | Online Article Text |
id | pubmed-5383825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Royal Society Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-53838252017-04-12 Concurrence in the ability for lipid synthesis between life stages in insects Visser, Bertanne Willett, Denis S. Harvey, Jeffrey A. Alborn, Hans T. R Soc Open Sci Biology (Whole Organism) The ability to synthesize lipids is critical for an organism’s fitness; hence, metabolic pathways, underlying lipid synthesis, tend to be highly conserved. Surprisingly, the majority of parasitoids deviate from this general metabolic model by lacking the ability to convert sugars and other carbohydrates into lipids. These insects spend the first part of their life feeding and developing in or on an arthropod host, during which they can carry over a substantial amount of lipid reserves. While many parasitoid species have been tested for lipogenic ability at the adult life stage, it has remained unclear whether parasitoid larvae can synthesize lipids. Here we investigate whether or not several insects can synthesize lipids during the larval stage using three ectoparasitic wasps (developing on the outside of the host) and the vinegar fly Drosophila melanogaster that differ in lipogenic ability in the adult life stage. Using feeding experiments and stable isotope tracing with gas chromatography/mass spectrometry, we first confirm lipogenic abilities in the adult life stage. Using topical application of stable isotopes in developing larvae, we then provide clear evidence of concurrence in lipogenic ability between larval and adult life stages in all species tested. The Royal Society Publishing 2017-03-22 /pmc/articles/PMC5383825/ /pubmed/28405368 http://dx.doi.org/10.1098/rsos.160815 Text en © 2017 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Biology (Whole Organism) Visser, Bertanne Willett, Denis S. Harvey, Jeffrey A. Alborn, Hans T. Concurrence in the ability for lipid synthesis between life stages in insects |
title | Concurrence in the ability for lipid synthesis between life stages in insects |
title_full | Concurrence in the ability for lipid synthesis between life stages in insects |
title_fullStr | Concurrence in the ability for lipid synthesis between life stages in insects |
title_full_unstemmed | Concurrence in the ability for lipid synthesis between life stages in insects |
title_short | Concurrence in the ability for lipid synthesis between life stages in insects |
title_sort | concurrence in the ability for lipid synthesis between life stages in insects |
topic | Biology (Whole Organism) |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383825/ https://www.ncbi.nlm.nih.gov/pubmed/28405368 http://dx.doi.org/10.1098/rsos.160815 |
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