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A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite
In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparati...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383914/ https://www.ncbi.nlm.nih.gov/pubmed/28395478 http://dx.doi.org/10.1186/s11671-017-2026-8 |
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author | Soldatkina, O. V. Soldatkin, O. O. Kasap, B. Ozansoy Kucherenko, D. Yu. Kucherenko, I. S. Kurc, B. Akata Dzyadevych, S. V. |
author_facet | Soldatkina, O. V. Soldatkin, O. O. Kasap, B. Ozansoy Kucherenko, D. Yu. Kucherenko, I. S. Kurc, B. Akata Dzyadevych, S. V. |
author_sort | Soldatkina, O. V. |
collection | PubMed |
description | In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples. |
format | Online Article Text |
id | pubmed-5383914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-53839142017-04-24 A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite Soldatkina, O. V. Soldatkin, O. O. Kasap, B. Ozansoy Kucherenko, D. Yu. Kucherenko, I. S. Kurc, B. Akata Dzyadevych, S. V. Nanoscale Res Lett Nano Express In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples. Springer US 2017-04-07 /pmc/articles/PMC5383914/ /pubmed/28395478 http://dx.doi.org/10.1186/s11671-017-2026-8 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Nano Express Soldatkina, O. V. Soldatkin, O. O. Kasap, B. Ozansoy Kucherenko, D. Yu. Kucherenko, I. S. Kurc, B. Akata Dzyadevych, S. V. A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite |
title | A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite |
title_full | A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite |
title_fullStr | A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite |
title_full_unstemmed | A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite |
title_short | A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite |
title_sort | novel amperometric glutamate biosensor based on glutamate oxidase adsorbed on silicalite |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383914/ https://www.ncbi.nlm.nih.gov/pubmed/28395478 http://dx.doi.org/10.1186/s11671-017-2026-8 |
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