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Effect of traditional Chinese medicine formula Sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study

BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) represents one of the most common forms of liver disease worldwide, and it is always regarded as a consequence of a sedentary, food-abundant lifestyle, sitting for an extended time, and a low physical activity level, which often coincide with chro...

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Autores principales: Cheng, Fafeng, Ma, Chongyang, Wang, Xueqian, Zhai, Changming, Wang, Guoli, Xu, Xiaolin, Mu, Jie, Li, Changxiang, Wang, Zisong, Zhang, Xiaoyu, Yue, Wenchao, Du, Xin, Lian, Yajun, Zhu, Wenxiang, Yin, Xiangjun, Wei, Zhen, Song, Wenjie, Wang, Qingguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383977/
https://www.ncbi.nlm.nih.gov/pubmed/28388904
http://dx.doi.org/10.1186/s12906-017-1707-2
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author Cheng, Fafeng
Ma, Chongyang
Wang, Xueqian
Zhai, Changming
Wang, Guoli
Xu, Xiaolin
Mu, Jie
Li, Changxiang
Wang, Zisong
Zhang, Xiaoyu
Yue, Wenchao
Du, Xin
Lian, Yajun
Zhu, Wenxiang
Yin, Xiangjun
Wei, Zhen
Song, Wenjie
Wang, Qingguo
author_facet Cheng, Fafeng
Ma, Chongyang
Wang, Xueqian
Zhai, Changming
Wang, Guoli
Xu, Xiaolin
Mu, Jie
Li, Changxiang
Wang, Zisong
Zhang, Xiaoyu
Yue, Wenchao
Du, Xin
Lian, Yajun
Zhu, Wenxiang
Yin, Xiangjun
Wei, Zhen
Song, Wenjie
Wang, Qingguo
author_sort Cheng, Fafeng
collection PubMed
description BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) represents one of the most common forms of liver disease worldwide, and it is always regarded as a consequence of a sedentary, food-abundant lifestyle, sitting for an extended time, and a low physical activity level, which often coincide with chronic and long-lasting psychological stress. A Chinese medicine Sinisan (SNS) may be a potential formula for treating this kind of disease. METHODS: In this study, a long-term chronic restraint stress protocol was used to investigate the mechanism underlying stress-induced NALFD. To investigate the effect of SNS treatment on stress-induced NAFLD, we measured the liver and serum values of total cholesterol (TC), triglyceride (TG), liver free fatty acids (FFA), low-density lipoprotein, superoxide dismutase, tumor necrosis factor-α, malondialdehyde, interleukin (IL)-6, and serum values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase. Results are shown as a mean ± standard deviation. Significant differences between the groups were evaluated using the Student t-test. For multiple comparisons, one-way analysis of variance (ANOVA) was used. If the results of ANOVA indicated significant differences, post hoc analysis was performed with the Tukey test or Dunnett test, and p < 0.05 was considered statistically significant. RESULTS: Long-term chronic stress led to steatosis and non-alcoholic steatohepatitis. Additionally, SNS treatment significantly increased body weight gain (p < 0.01) and sucrose preference (p < 0.001), and it reduced the liver values of TC, TG, and FFA (p < 0.05). SNS also reduced the serum values of AST and ALT (p < 0.001), and the liver value of IL-6 (p < 0.01). CONCLUSIONS: This study’s results demonstrate that psychological stress may be a significant risk factor of NAFLD. Furthermore, the traditional Chinese medicine formula SNS may have some beneficial effect in antagonizing psychological stress and stress-related NAFLD.
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spelling pubmed-53839772017-04-10 Effect of traditional Chinese medicine formula Sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study Cheng, Fafeng Ma, Chongyang Wang, Xueqian Zhai, Changming Wang, Guoli Xu, Xiaolin Mu, Jie Li, Changxiang Wang, Zisong Zhang, Xiaoyu Yue, Wenchao Du, Xin Lian, Yajun Zhu, Wenxiang Yin, Xiangjun Wei, Zhen Song, Wenjie Wang, Qingguo BMC Complement Altern Med Research Article BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) represents one of the most common forms of liver disease worldwide, and it is always regarded as a consequence of a sedentary, food-abundant lifestyle, sitting for an extended time, and a low physical activity level, which often coincide with chronic and long-lasting psychological stress. A Chinese medicine Sinisan (SNS) may be a potential formula for treating this kind of disease. METHODS: In this study, a long-term chronic restraint stress protocol was used to investigate the mechanism underlying stress-induced NALFD. To investigate the effect of SNS treatment on stress-induced NAFLD, we measured the liver and serum values of total cholesterol (TC), triglyceride (TG), liver free fatty acids (FFA), low-density lipoprotein, superoxide dismutase, tumor necrosis factor-α, malondialdehyde, interleukin (IL)-6, and serum values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase. Results are shown as a mean ± standard deviation. Significant differences between the groups were evaluated using the Student t-test. For multiple comparisons, one-way analysis of variance (ANOVA) was used. If the results of ANOVA indicated significant differences, post hoc analysis was performed with the Tukey test or Dunnett test, and p < 0.05 was considered statistically significant. RESULTS: Long-term chronic stress led to steatosis and non-alcoholic steatohepatitis. Additionally, SNS treatment significantly increased body weight gain (p < 0.01) and sucrose preference (p < 0.001), and it reduced the liver values of TC, TG, and FFA (p < 0.05). SNS also reduced the serum values of AST and ALT (p < 0.001), and the liver value of IL-6 (p < 0.01). CONCLUSIONS: This study’s results demonstrate that psychological stress may be a significant risk factor of NAFLD. Furthermore, the traditional Chinese medicine formula SNS may have some beneficial effect in antagonizing psychological stress and stress-related NAFLD. BioMed Central 2017-04-07 /pmc/articles/PMC5383977/ /pubmed/28388904 http://dx.doi.org/10.1186/s12906-017-1707-2 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Cheng, Fafeng
Ma, Chongyang
Wang, Xueqian
Zhai, Changming
Wang, Guoli
Xu, Xiaolin
Mu, Jie
Li, Changxiang
Wang, Zisong
Zhang, Xiaoyu
Yue, Wenchao
Du, Xin
Lian, Yajun
Zhu, Wenxiang
Yin, Xiangjun
Wei, Zhen
Song, Wenjie
Wang, Qingguo
Effect of traditional Chinese medicine formula Sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study
title Effect of traditional Chinese medicine formula Sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study
title_full Effect of traditional Chinese medicine formula Sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study
title_fullStr Effect of traditional Chinese medicine formula Sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study
title_full_unstemmed Effect of traditional Chinese medicine formula Sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study
title_short Effect of traditional Chinese medicine formula Sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study
title_sort effect of traditional chinese medicine formula sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383977/
https://www.ncbi.nlm.nih.gov/pubmed/28388904
http://dx.doi.org/10.1186/s12906-017-1707-2
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