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Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China

BACKGROUND: Hirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe. METHODS: In this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong provinc...

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Autores principales: Zhang, Jialin, Tang, Xiaoqian, Sheng, Xiuzhen, Xing, Jing, Zhan, Wenbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384145/
https://www.ncbi.nlm.nih.gov/pubmed/28388934
http://dx.doi.org/10.1186/s12985-017-0742-4
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author Zhang, Jialin
Tang, Xiaoqian
Sheng, Xiuzhen
Xing, Jing
Zhan, Wenbin
author_facet Zhang, Jialin
Tang, Xiaoqian
Sheng, Xiuzhen
Xing, Jing
Zhan, Wenbin
author_sort Zhang, Jialin
collection PubMed
description BACKGROUND: Hirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe. METHODS: In this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong province, China. Cell isolation, electron microscopic observation, RT-PCR detection and phylogenetic analysis were used for virus identification. Further, artificial infection experiment was conducted for virulence testing. RESULTS: The gross signs included abdominal distension, fin reddening and yellow ascitic fluid in the abdominal cavity. Histopathological examination revealed marked cell degeneration and necrosis in the kidney. The tissue homogenates induced obvious cytopathic effects in EPC, FHM and FG cell lines. Electron microscopic observation showed the virus had a bullet-like shape with a capsule membrane. RT-PCR and sequencing analysis revealed that CNPo2015 belonged to the HIRRV with high sequence identity to HIRRV isolates. Infection experiment confirmed that the HIRRV CNPo2015 strain was virulent to flounder juveniles with a LD(50) value of 1.0 × 10(5.9) TCID(50)/fish. CONCLUSION: In conclusion, we described the first isolation and characterization of a HIRRV from Japanese flounder in China. This will provide a candidate material for further research on the infection mechanism and preventive strategies of HIRRV.
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spelling pubmed-53841452017-04-12 Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China Zhang, Jialin Tang, Xiaoqian Sheng, Xiuzhen Xing, Jing Zhan, Wenbin Virol J Research BACKGROUND: Hirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe. METHODS: In this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong province, China. Cell isolation, electron microscopic observation, RT-PCR detection and phylogenetic analysis were used for virus identification. Further, artificial infection experiment was conducted for virulence testing. RESULTS: The gross signs included abdominal distension, fin reddening and yellow ascitic fluid in the abdominal cavity. Histopathological examination revealed marked cell degeneration and necrosis in the kidney. The tissue homogenates induced obvious cytopathic effects in EPC, FHM and FG cell lines. Electron microscopic observation showed the virus had a bullet-like shape with a capsule membrane. RT-PCR and sequencing analysis revealed that CNPo2015 belonged to the HIRRV with high sequence identity to HIRRV isolates. Infection experiment confirmed that the HIRRV CNPo2015 strain was virulent to flounder juveniles with a LD(50) value of 1.0 × 10(5.9) TCID(50)/fish. CONCLUSION: In conclusion, we described the first isolation and characterization of a HIRRV from Japanese flounder in China. This will provide a candidate material for further research on the infection mechanism and preventive strategies of HIRRV. BioMed Central 2017-04-07 /pmc/articles/PMC5384145/ /pubmed/28388934 http://dx.doi.org/10.1186/s12985-017-0742-4 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhang, Jialin
Tang, Xiaoqian
Sheng, Xiuzhen
Xing, Jing
Zhan, Wenbin
Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China
title Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China
title_full Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China
title_fullStr Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China
title_full_unstemmed Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China
title_short Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China
title_sort isolation and identification of a new strain of hirame rhabdovirus (hirrv) from japanese flounder paralichthys olivaceus in china
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384145/
https://www.ncbi.nlm.nih.gov/pubmed/28388934
http://dx.doi.org/10.1186/s12985-017-0742-4
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