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Retinoic Acid Mediates Visceral-Specific Adipogenic Defects of Human Adipose-Derived Stem Cells
Increased visceral fat, rather than subcutaneous fat, during the onset of obesity is associated with a higher risk of developing metabolic diseases. The inherent adipogenic properties of human adipose-derived stem cells (ASCs) from visceral depots are compromised compared with those of ASCs from sub...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Diabetes Association
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384626/ https://www.ncbi.nlm.nih.gov/pubmed/26936961 http://dx.doi.org/10.2337/db15-1315 |
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author | Takeda, Kosuke Sriram, Sandhya Chan, Xin Hui Derryn Ong, Wee Kiat Yeo, Chia Rou Tan, Betty Lee, Seung-Ah Kong, Kien Voon Hoon, Shawn Jiang, Hongfeng Yuen, Jason J. Perumal, Jayakumar Agrawal, Madhur Vaz, Candida So, Jimmy Shabbir, Asim Blaner, William S. Olivo, Malini Han, Weiping Tanavde, Vivek Toh, Sue-Anne Sugii, Shigeki |
author_facet | Takeda, Kosuke Sriram, Sandhya Chan, Xin Hui Derryn Ong, Wee Kiat Yeo, Chia Rou Tan, Betty Lee, Seung-Ah Kong, Kien Voon Hoon, Shawn Jiang, Hongfeng Yuen, Jason J. Perumal, Jayakumar Agrawal, Madhur Vaz, Candida So, Jimmy Shabbir, Asim Blaner, William S. Olivo, Malini Han, Weiping Tanavde, Vivek Toh, Sue-Anne Sugii, Shigeki |
author_sort | Takeda, Kosuke |
collection | PubMed |
description | Increased visceral fat, rather than subcutaneous fat, during the onset of obesity is associated with a higher risk of developing metabolic diseases. The inherent adipogenic properties of human adipose-derived stem cells (ASCs) from visceral depots are compromised compared with those of ASCs from subcutaneous depots, but little is known about the underlying mechanisms. Using ontological analysis of global gene expression studies, we demonstrate that many genes involved in retinoic acid (RA) synthesis or regulated by RA are differentially expressed in human tissues and ASCs from subcutaneous and visceral fat. The endogenous level of RA is higher in visceral ASCs; this is associated with upregulation of the RA synthesis gene through the visceral-specific developmental factor WT1. Excessive RA-mediated activity impedes the adipogenic capability of ASCs at early but not late stages of adipogenesis, which can be reversed by antagonism of RA receptors or knockdown of WT1. Our results reveal the developmental origin of adipocytic properties and the pathophysiological contributions of visceral fat depots. |
format | Online Article Text |
id | pubmed-5384626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | American Diabetes Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-53846262017-05-01 Retinoic Acid Mediates Visceral-Specific Adipogenic Defects of Human Adipose-Derived Stem Cells Takeda, Kosuke Sriram, Sandhya Chan, Xin Hui Derryn Ong, Wee Kiat Yeo, Chia Rou Tan, Betty Lee, Seung-Ah Kong, Kien Voon Hoon, Shawn Jiang, Hongfeng Yuen, Jason J. Perumal, Jayakumar Agrawal, Madhur Vaz, Candida So, Jimmy Shabbir, Asim Blaner, William S. Olivo, Malini Han, Weiping Tanavde, Vivek Toh, Sue-Anne Sugii, Shigeki Diabetes Metabolism Increased visceral fat, rather than subcutaneous fat, during the onset of obesity is associated with a higher risk of developing metabolic diseases. The inherent adipogenic properties of human adipose-derived stem cells (ASCs) from visceral depots are compromised compared with those of ASCs from subcutaneous depots, but little is known about the underlying mechanisms. Using ontological analysis of global gene expression studies, we demonstrate that many genes involved in retinoic acid (RA) synthesis or regulated by RA are differentially expressed in human tissues and ASCs from subcutaneous and visceral fat. The endogenous level of RA is higher in visceral ASCs; this is associated with upregulation of the RA synthesis gene through the visceral-specific developmental factor WT1. Excessive RA-mediated activity impedes the adipogenic capability of ASCs at early but not late stages of adipogenesis, which can be reversed by antagonism of RA receptors or knockdown of WT1. Our results reveal the developmental origin of adipocytic properties and the pathophysiological contributions of visceral fat depots. American Diabetes Association 2016-05 2016-03-02 /pmc/articles/PMC5384626/ /pubmed/26936961 http://dx.doi.org/10.2337/db15-1315 Text en © 2016 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. |
spellingShingle | Metabolism Takeda, Kosuke Sriram, Sandhya Chan, Xin Hui Derryn Ong, Wee Kiat Yeo, Chia Rou Tan, Betty Lee, Seung-Ah Kong, Kien Voon Hoon, Shawn Jiang, Hongfeng Yuen, Jason J. Perumal, Jayakumar Agrawal, Madhur Vaz, Candida So, Jimmy Shabbir, Asim Blaner, William S. Olivo, Malini Han, Weiping Tanavde, Vivek Toh, Sue-Anne Sugii, Shigeki Retinoic Acid Mediates Visceral-Specific Adipogenic Defects of Human Adipose-Derived Stem Cells |
title | Retinoic Acid Mediates Visceral-Specific Adipogenic Defects of Human Adipose-Derived Stem Cells |
title_full | Retinoic Acid Mediates Visceral-Specific Adipogenic Defects of Human Adipose-Derived Stem Cells |
title_fullStr | Retinoic Acid Mediates Visceral-Specific Adipogenic Defects of Human Adipose-Derived Stem Cells |
title_full_unstemmed | Retinoic Acid Mediates Visceral-Specific Adipogenic Defects of Human Adipose-Derived Stem Cells |
title_short | Retinoic Acid Mediates Visceral-Specific Adipogenic Defects of Human Adipose-Derived Stem Cells |
title_sort | retinoic acid mediates visceral-specific adipogenic defects of human adipose-derived stem cells |
topic | Metabolism |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384626/ https://www.ncbi.nlm.nih.gov/pubmed/26936961 http://dx.doi.org/10.2337/db15-1315 |
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