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Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems

It is generally accepted that diverse, poorly characterized microorganisms reside deep within Earth’s crust. One such lineage of deep subsurface-dwelling bacteria is an uncultivated member of the Firmicutes phylum that can dominate molecular surveys from both marine and continental rock fracture flu...

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Autores principales: Jungbluth, Sean P., Glavina del Rio, Tijana, Tringe, Susannah G., Stepanauskas, Ramunas, Rappé, Michael S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385130/
https://www.ncbi.nlm.nih.gov/pubmed/28396823
http://dx.doi.org/10.7717/peerj.3134
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author Jungbluth, Sean P.
Glavina del Rio, Tijana
Tringe, Susannah G.
Stepanauskas, Ramunas
Rappé, Michael S.
author_facet Jungbluth, Sean P.
Glavina del Rio, Tijana
Tringe, Susannah G.
Stepanauskas, Ramunas
Rappé, Michael S.
author_sort Jungbluth, Sean P.
collection PubMed
description It is generally accepted that diverse, poorly characterized microorganisms reside deep within Earth’s crust. One such lineage of deep subsurface-dwelling bacteria is an uncultivated member of the Firmicutes phylum that can dominate molecular surveys from both marine and continental rock fracture fluids, sometimes forming the sole member of a single-species microbiome. Here, we reconstructed a genome from basalt-hosted fluids of the deep subseafloor along the eastern Juan de Fuca Ridge flank and used a phylogenomic analysis to show that, despite vast differences in geographic origin and habitat, it forms a monophyletic clade with the terrestrial deep subsurface genome of “Candidatus Desulforudis audaxviator” MP104C. While a limited number of differences were observed between the marine genome of “Candidatus Desulfopertinax cowenii” modA32 and its terrestrial relative that may be of potential adaptive importance, here it is revealed that the two are remarkably similar thermophiles possessing the genetic capacity for motility, sporulation, hydrogenotrophy, chemoorganotrophy, dissimilatory sulfate reduction, and the ability to fix inorganic carbon via the Wood-Ljungdahl pathway for chemoautotrophic growth. Our results provide insights into the genetic repertoire within marine and terrestrial members of a bacterial lineage that is widespread in the global deep subsurface biosphere, and provides a natural means to investigate adaptations specific to these two environments.
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spelling pubmed-53851302017-04-10 Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems Jungbluth, Sean P. Glavina del Rio, Tijana Tringe, Susannah G. Stepanauskas, Ramunas Rappé, Michael S. PeerJ Ecology It is generally accepted that diverse, poorly characterized microorganisms reside deep within Earth’s crust. One such lineage of deep subsurface-dwelling bacteria is an uncultivated member of the Firmicutes phylum that can dominate molecular surveys from both marine and continental rock fracture fluids, sometimes forming the sole member of a single-species microbiome. Here, we reconstructed a genome from basalt-hosted fluids of the deep subseafloor along the eastern Juan de Fuca Ridge flank and used a phylogenomic analysis to show that, despite vast differences in geographic origin and habitat, it forms a monophyletic clade with the terrestrial deep subsurface genome of “Candidatus Desulforudis audaxviator” MP104C. While a limited number of differences were observed between the marine genome of “Candidatus Desulfopertinax cowenii” modA32 and its terrestrial relative that may be of potential adaptive importance, here it is revealed that the two are remarkably similar thermophiles possessing the genetic capacity for motility, sporulation, hydrogenotrophy, chemoorganotrophy, dissimilatory sulfate reduction, and the ability to fix inorganic carbon via the Wood-Ljungdahl pathway for chemoautotrophic growth. Our results provide insights into the genetic repertoire within marine and terrestrial members of a bacterial lineage that is widespread in the global deep subsurface biosphere, and provides a natural means to investigate adaptations specific to these two environments. PeerJ Inc. 2017-04-06 /pmc/articles/PMC5385130/ /pubmed/28396823 http://dx.doi.org/10.7717/peerj.3134 Text en ©2017 Jungbluth et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Ecology
Jungbluth, Sean P.
Glavina del Rio, Tijana
Tringe, Susannah G.
Stepanauskas, Ramunas
Rappé, Michael S.
Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems
title Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems
title_full Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems
title_fullStr Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems
title_full_unstemmed Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems
title_short Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems
title_sort genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems
topic Ecology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385130/
https://www.ncbi.nlm.nih.gov/pubmed/28396823
http://dx.doi.org/10.7717/peerj.3134
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