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Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress

Phosphorus (P) is a vital nutrient for plant growth and development, and is absorbed in cells with the help of membrane-spanning inorganic phosphate transporter (Pht) protein. Symbiosis with arbuscular mycorrhiza (AM) also helps in transporting P from the soil to plant and Pht proteins play an impor...

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Autores principales: Pudake, Ramesh Namdeo, Mehta, Chandra Mohan, Mohanta, Tapan Kumar, Sharma, Suvigya, Varma, Ajit, Sharma, Anil Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385181/
https://www.ncbi.nlm.nih.gov/pubmed/28391483
http://dx.doi.org/10.1007/s13205-017-0609-9
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author Pudake, Ramesh Namdeo
Mehta, Chandra Mohan
Mohanta, Tapan Kumar
Sharma, Suvigya
Varma, Ajit
Sharma, Anil Kumar
author_facet Pudake, Ramesh Namdeo
Mehta, Chandra Mohan
Mohanta, Tapan Kumar
Sharma, Suvigya
Varma, Ajit
Sharma, Anil Kumar
author_sort Pudake, Ramesh Namdeo
collection PubMed
description Phosphorus (P) is a vital nutrient for plant growth and development, and is absorbed in cells with the help of membrane-spanning inorganic phosphate transporter (Pht) protein. Symbiosis with arbuscular mycorrhiza (AM) also helps in transporting P from the soil to plant and Pht proteins play an important role in it. To understand this phenomenon in Finger Mille plant, we have cloned four Pht genes from Finger millet, which shares the homology with Pht1 protein family of cereals. Expression pattern analysis during the AM infection indicated that EcPT4 gene was AM specific, and its expression was higher in roots where AM colonization percentage was high. The expression level of EcPT1-4 gene under the phosphorous (Pi) stress in seedlings was found to be consistent with its role in acquisition of phosphorus. Homology study of the EcPt proteins with Pht proteins of cereals shows close relationship. The findings of the study indicate that Pht1 family genes from finger millet can serve to be an important resource for the better understanding of phosphorus use efficiency.
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spelling pubmed-53851812017-04-20 Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress Pudake, Ramesh Namdeo Mehta, Chandra Mohan Mohanta, Tapan Kumar Sharma, Suvigya Varma, Ajit Sharma, Anil Kumar 3 Biotech Original Article Phosphorus (P) is a vital nutrient for plant growth and development, and is absorbed in cells with the help of membrane-spanning inorganic phosphate transporter (Pht) protein. Symbiosis with arbuscular mycorrhiza (AM) also helps in transporting P from the soil to plant and Pht proteins play an important role in it. To understand this phenomenon in Finger Mille plant, we have cloned four Pht genes from Finger millet, which shares the homology with Pht1 protein family of cereals. Expression pattern analysis during the AM infection indicated that EcPT4 gene was AM specific, and its expression was higher in roots where AM colonization percentage was high. The expression level of EcPT1-4 gene under the phosphorous (Pi) stress in seedlings was found to be consistent with its role in acquisition of phosphorus. Homology study of the EcPt proteins with Pht proteins of cereals shows close relationship. The findings of the study indicate that Pht1 family genes from finger millet can serve to be an important resource for the better understanding of phosphorus use efficiency. Springer Berlin Heidelberg 2017-04-08 2017-05 /pmc/articles/PMC5385181/ /pubmed/28391483 http://dx.doi.org/10.1007/s13205-017-0609-9 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Pudake, Ramesh Namdeo
Mehta, Chandra Mohan
Mohanta, Tapan Kumar
Sharma, Suvigya
Varma, Ajit
Sharma, Anil Kumar
Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress
title Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress
title_full Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress
title_fullStr Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress
title_full_unstemmed Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress
title_short Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress
title_sort expression of four phosphate transporter genes from finger millet (eleusine coracana l.) in response to mycorrhizal colonization and pi stress
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385181/
https://www.ncbi.nlm.nih.gov/pubmed/28391483
http://dx.doi.org/10.1007/s13205-017-0609-9
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