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Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress
Phosphorus (P) is a vital nutrient for plant growth and development, and is absorbed in cells with the help of membrane-spanning inorganic phosphate transporter (Pht) protein. Symbiosis with arbuscular mycorrhiza (AM) also helps in transporting P from the soil to plant and Pht proteins play an impor...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385181/ https://www.ncbi.nlm.nih.gov/pubmed/28391483 http://dx.doi.org/10.1007/s13205-017-0609-9 |
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author | Pudake, Ramesh Namdeo Mehta, Chandra Mohan Mohanta, Tapan Kumar Sharma, Suvigya Varma, Ajit Sharma, Anil Kumar |
author_facet | Pudake, Ramesh Namdeo Mehta, Chandra Mohan Mohanta, Tapan Kumar Sharma, Suvigya Varma, Ajit Sharma, Anil Kumar |
author_sort | Pudake, Ramesh Namdeo |
collection | PubMed |
description | Phosphorus (P) is a vital nutrient for plant growth and development, and is absorbed in cells with the help of membrane-spanning inorganic phosphate transporter (Pht) protein. Symbiosis with arbuscular mycorrhiza (AM) also helps in transporting P from the soil to plant and Pht proteins play an important role in it. To understand this phenomenon in Finger Mille plant, we have cloned four Pht genes from Finger millet, which shares the homology with Pht1 protein family of cereals. Expression pattern analysis during the AM infection indicated that EcPT4 gene was AM specific, and its expression was higher in roots where AM colonization percentage was high. The expression level of EcPT1-4 gene under the phosphorous (Pi) stress in seedlings was found to be consistent with its role in acquisition of phosphorus. Homology study of the EcPt proteins with Pht proteins of cereals shows close relationship. The findings of the study indicate that Pht1 family genes from finger millet can serve to be an important resource for the better understanding of phosphorus use efficiency. |
format | Online Article Text |
id | pubmed-5385181 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-53851812017-04-20 Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress Pudake, Ramesh Namdeo Mehta, Chandra Mohan Mohanta, Tapan Kumar Sharma, Suvigya Varma, Ajit Sharma, Anil Kumar 3 Biotech Original Article Phosphorus (P) is a vital nutrient for plant growth and development, and is absorbed in cells with the help of membrane-spanning inorganic phosphate transporter (Pht) protein. Symbiosis with arbuscular mycorrhiza (AM) also helps in transporting P from the soil to plant and Pht proteins play an important role in it. To understand this phenomenon in Finger Mille plant, we have cloned four Pht genes from Finger millet, which shares the homology with Pht1 protein family of cereals. Expression pattern analysis during the AM infection indicated that EcPT4 gene was AM specific, and its expression was higher in roots where AM colonization percentage was high. The expression level of EcPT1-4 gene under the phosphorous (Pi) stress in seedlings was found to be consistent with its role in acquisition of phosphorus. Homology study of the EcPt proteins with Pht proteins of cereals shows close relationship. The findings of the study indicate that Pht1 family genes from finger millet can serve to be an important resource for the better understanding of phosphorus use efficiency. Springer Berlin Heidelberg 2017-04-08 2017-05 /pmc/articles/PMC5385181/ /pubmed/28391483 http://dx.doi.org/10.1007/s13205-017-0609-9 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Pudake, Ramesh Namdeo Mehta, Chandra Mohan Mohanta, Tapan Kumar Sharma, Suvigya Varma, Ajit Sharma, Anil Kumar Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress |
title | Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress |
title_full | Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress |
title_fullStr | Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress |
title_full_unstemmed | Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress |
title_short | Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress |
title_sort | expression of four phosphate transporter genes from finger millet (eleusine coracana l.) in response to mycorrhizal colonization and pi stress |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385181/ https://www.ncbi.nlm.nih.gov/pubmed/28391483 http://dx.doi.org/10.1007/s13205-017-0609-9 |
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